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Overexpression of the CC-type glutaredoxin, OsGRX6 affects hormone and nitrogen status in rice plants

Glutaredoxins (GRXs) are small glutathione dependent oxidoreductases that belong to the Thioredoxin (TRX) superfamily and catalyze the reduction of disulfide bonds of their substrate proteins. Plant GRXs include three different groups based on the motif sequence, namely CPYC, CGFS, and CC-type prote...

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Autores principales: El-Kereamy, Ashraf, Bi, Yong-Mei, Mahmood, Kashif, Ranathunge, Kosala, Yaish, Mahmoud W., Nambara, Eiji, Rothstein, Steven J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4630655/
https://www.ncbi.nlm.nih.gov/pubmed/26579177
http://dx.doi.org/10.3389/fpls.2015.00934
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author El-Kereamy, Ashraf
Bi, Yong-Mei
Mahmood, Kashif
Ranathunge, Kosala
Yaish, Mahmoud W.
Nambara, Eiji
Rothstein, Steven J.
author_facet El-Kereamy, Ashraf
Bi, Yong-Mei
Mahmood, Kashif
Ranathunge, Kosala
Yaish, Mahmoud W.
Nambara, Eiji
Rothstein, Steven J.
author_sort El-Kereamy, Ashraf
collection PubMed
description Glutaredoxins (GRXs) are small glutathione dependent oxidoreductases that belong to the Thioredoxin (TRX) superfamily and catalyze the reduction of disulfide bonds of their substrate proteins. Plant GRXs include three different groups based on the motif sequence, namely CPYC, CGFS, and CC-type proteins. The rice CC-type proteins, OsGRX6 was identified during the screening for genes whose expression changes depending on the level of available nitrate. Overexpression of OsGRX6 in rice displayed a semi-dwarf phenotype. The OsGRX6 overexpressors contain a higher nitrogen content than the wild type, indicating that OsGRX6 plays a role in homeostatic regulation of nitrogen use. Consistent with this, OsGRX6 overexpressors displayed delayed chlorophyll degradation and senescence compared to the wild type plants. To examine if the growth defect of these transgenic lines attribute to disturbed plant hormone actions, plant hormone levels were measured. The levels of two cytokinins (CKs), 2-isopentenyladenine and trans-zeatin, and gibberellin A1 (GA1) were increased in these lines. We also found that these transgenic lines were less sensitive to exogenously applied GA, suggesting that the increase in GA1 is a result of the feedback regulation. These data suggest that OsGRX6 affects hormone signaling and nitrogen status in rice plants.
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spelling pubmed-46306552015-11-17 Overexpression of the CC-type glutaredoxin, OsGRX6 affects hormone and nitrogen status in rice plants El-Kereamy, Ashraf Bi, Yong-Mei Mahmood, Kashif Ranathunge, Kosala Yaish, Mahmoud W. Nambara, Eiji Rothstein, Steven J. Front Plant Sci Plant Science Glutaredoxins (GRXs) are small glutathione dependent oxidoreductases that belong to the Thioredoxin (TRX) superfamily and catalyze the reduction of disulfide bonds of their substrate proteins. Plant GRXs include three different groups based on the motif sequence, namely CPYC, CGFS, and CC-type proteins. The rice CC-type proteins, OsGRX6 was identified during the screening for genes whose expression changes depending on the level of available nitrate. Overexpression of OsGRX6 in rice displayed a semi-dwarf phenotype. The OsGRX6 overexpressors contain a higher nitrogen content than the wild type, indicating that OsGRX6 plays a role in homeostatic regulation of nitrogen use. Consistent with this, OsGRX6 overexpressors displayed delayed chlorophyll degradation and senescence compared to the wild type plants. To examine if the growth defect of these transgenic lines attribute to disturbed plant hormone actions, plant hormone levels were measured. The levels of two cytokinins (CKs), 2-isopentenyladenine and trans-zeatin, and gibberellin A1 (GA1) were increased in these lines. We also found that these transgenic lines were less sensitive to exogenously applied GA, suggesting that the increase in GA1 is a result of the feedback regulation. These data suggest that OsGRX6 affects hormone signaling and nitrogen status in rice plants. Frontiers Media S.A. 2015-11-03 /pmc/articles/PMC4630655/ /pubmed/26579177 http://dx.doi.org/10.3389/fpls.2015.00934 Text en Copyright © 2015 El-Kereamy, Bi, Mahmood, Ranathunge, Yaish, Nambara and Rothstein. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
El-Kereamy, Ashraf
Bi, Yong-Mei
Mahmood, Kashif
Ranathunge, Kosala
Yaish, Mahmoud W.
Nambara, Eiji
Rothstein, Steven J.
Overexpression of the CC-type glutaredoxin, OsGRX6 affects hormone and nitrogen status in rice plants
title Overexpression of the CC-type glutaredoxin, OsGRX6 affects hormone and nitrogen status in rice plants
title_full Overexpression of the CC-type glutaredoxin, OsGRX6 affects hormone and nitrogen status in rice plants
title_fullStr Overexpression of the CC-type glutaredoxin, OsGRX6 affects hormone and nitrogen status in rice plants
title_full_unstemmed Overexpression of the CC-type glutaredoxin, OsGRX6 affects hormone and nitrogen status in rice plants
title_short Overexpression of the CC-type glutaredoxin, OsGRX6 affects hormone and nitrogen status in rice plants
title_sort overexpression of the cc-type glutaredoxin, osgrx6 affects hormone and nitrogen status in rice plants
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4630655/
https://www.ncbi.nlm.nih.gov/pubmed/26579177
http://dx.doi.org/10.3389/fpls.2015.00934
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