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A cytomorphometric analysis of oral mucosal changes in tobacco users
AIM: Tobacco use is the major cause of oral cancer, which is the sixth most common form of malignancy globally. Even in the absence of clinical manifestations, early changes in the oral mucosa can be detected microscopically by exfoliative cytology. The present study aimed to study and compare the c...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4630757/ https://www.ncbi.nlm.nih.gov/pubmed/26604613 http://dx.doi.org/10.4103/0976-9668.166055 |
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author | Khot, Komal Deshmane, Swati Bagri-Manjarekar, Kriti Warke, Darshana Kotak, Keyuri |
author_facet | Khot, Komal Deshmane, Swati Bagri-Manjarekar, Kriti Warke, Darshana Kotak, Keyuri |
author_sort | Khot, Komal |
collection | PubMed |
description | AIM: Tobacco use is the major cause of oral cancer, which is the sixth most common form of malignancy globally. Even in the absence of clinical manifestations, early changes in the oral mucosa can be detected microscopically by exfoliative cytology. The present study aimed to study and compare the cellular changes in the oral mucosa of tobacco users using cytomorphometry. MATERIALS AND METHODS: A total of 80 subjects were included: 20 without any tobacco use habits, 20 tobacco chewers, 20 smokers, and 20 mishri users. Smears were collected from each subject, fixed, and stained using Papanicolaou stain. All slides were evaluated for nuclear and cytoplasmic changes using image analysis software. RESULTS: Statistically significant decrease in cytoplasmic area and an increase in nuclear area and nuclear-cytoplasmic ratio were observed in tobacco users. CONCLUSION: Tobacco in any form causes significant cellular changes, which could be the earliest indicators of developing malignant pathology. Exfoliative cytology can be used to detect such changes. |
format | Online Article Text |
id | pubmed-4630757 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-46307572015-11-24 A cytomorphometric analysis of oral mucosal changes in tobacco users Khot, Komal Deshmane, Swati Bagri-Manjarekar, Kriti Warke, Darshana Kotak, Keyuri J Nat Sci Biol Med Original Article AIM: Tobacco use is the major cause of oral cancer, which is the sixth most common form of malignancy globally. Even in the absence of clinical manifestations, early changes in the oral mucosa can be detected microscopically by exfoliative cytology. The present study aimed to study and compare the cellular changes in the oral mucosa of tobacco users using cytomorphometry. MATERIALS AND METHODS: A total of 80 subjects were included: 20 without any tobacco use habits, 20 tobacco chewers, 20 smokers, and 20 mishri users. Smears were collected from each subject, fixed, and stained using Papanicolaou stain. All slides were evaluated for nuclear and cytoplasmic changes using image analysis software. RESULTS: Statistically significant decrease in cytoplasmic area and an increase in nuclear area and nuclear-cytoplasmic ratio were observed in tobacco users. CONCLUSION: Tobacco in any form causes significant cellular changes, which could be the earliest indicators of developing malignant pathology. Exfoliative cytology can be used to detect such changes. Medknow Publications & Media Pvt Ltd 2015-08 /pmc/articles/PMC4630757/ /pubmed/26604613 http://dx.doi.org/10.4103/0976-9668.166055 Text en Copyright: © Journal of Natural Science, Biology and Medicine http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Khot, Komal Deshmane, Swati Bagri-Manjarekar, Kriti Warke, Darshana Kotak, Keyuri A cytomorphometric analysis of oral mucosal changes in tobacco users |
title | A cytomorphometric analysis of oral mucosal changes in tobacco users |
title_full | A cytomorphometric analysis of oral mucosal changes in tobacco users |
title_fullStr | A cytomorphometric analysis of oral mucosal changes in tobacco users |
title_full_unstemmed | A cytomorphometric analysis of oral mucosal changes in tobacco users |
title_short | A cytomorphometric analysis of oral mucosal changes in tobacco users |
title_sort | cytomorphometric analysis of oral mucosal changes in tobacco users |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4630757/ https://www.ncbi.nlm.nih.gov/pubmed/26604613 http://dx.doi.org/10.4103/0976-9668.166055 |
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