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Cnidaria: fast, reference-free clustering of raw and assembled genome and transcriptome NGS data

BACKGROUND: Identification of biological specimens is a requirement for a range of applications. Reference-free methods analyse unprocessed sequencing data without relying on prior knowledge, but generally do not scale to arbitrarily large genomes and arbitrarily large phylogenetic distances. RESULT...

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Detalles Bibliográficos
Autores principales: Aflitos, Saulo Alves, Severing, Edouard, Sanchez-Perez, Gabino, Peters, Sander, de Jong, Hans, de Ridder, Dick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4630969/
https://www.ncbi.nlm.nih.gov/pubmed/26525298
http://dx.doi.org/10.1186/s12859-015-0806-7
Descripción
Sumario:BACKGROUND: Identification of biological specimens is a requirement for a range of applications. Reference-free methods analyse unprocessed sequencing data without relying on prior knowledge, but generally do not scale to arbitrarily large genomes and arbitrarily large phylogenetic distances. RESULTS: We present Cnidaria, a practical tool for clustering genomic and transcriptomic data with no limitation on genome size or phylogenetic distances. We successfully simultaneously clustered 169 genomic and transcriptomic datasets from 4 kingdoms, achieving 100 % identification accuracy at supra-species level and 78 % accuracy at the species level. CONCLUSION: CNIDARIA allows for fast, resource-efficient comparison and identification of both raw and assembled genome and transcriptome data. This can help answer both fundamental (e.g. in phylogeny, ecological diversity analysis) and practical questions (e.g. sequencing quality control, primer design). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12859-015-0806-7) contains supplementary material, which is available to authorized users.