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Genome-Wide Association Implicates Candidate Genes Conferring Resistance to Maize Rough Dwarf Disease in Maize

Maize rough dwarf disease (MRDD) is a destructive viral disease in China, which results in 20–30% of the maize yield losses in affected areas and even as high as 100% in severely infected fields. Understanding the genetic basis of resistance will provide important insights for maize breeding program...

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Autores principales: Chen, Gengshen, Wang, Xiaoming, Hao, Junjie, Yan, Jianbing, Ding, Junqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4631334/
https://www.ncbi.nlm.nih.gov/pubmed/26529245
http://dx.doi.org/10.1371/journal.pone.0142001
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author Chen, Gengshen
Wang, Xiaoming
Hao, Junjie
Yan, Jianbing
Ding, Junqiang
author_facet Chen, Gengshen
Wang, Xiaoming
Hao, Junjie
Yan, Jianbing
Ding, Junqiang
author_sort Chen, Gengshen
collection PubMed
description Maize rough dwarf disease (MRDD) is a destructive viral disease in China, which results in 20–30% of the maize yield losses in affected areas and even as high as 100% in severely infected fields. Understanding the genetic basis of resistance will provide important insights for maize breeding program. In this study, a diverse maize population comprising of 527 inbred lines was evaluated in four environments and a genome-wide association study (GWAS) was undertaken with over 556000 SNP markers. Fifteen candidate genes associated with MRDD resistance were identified, including ten genes with annotated protein encoding functions. The homologous of nine candidate genes were predicted to relate to plant defense in different species based on published results. Significant correlation (R(2) = 0.79) between the MRDD severity and the number of resistance alleles was observed. Consequently, we have broadened the resistant germplasm to MRDD and identified a number of resistance alleles by GWAS. The results in present study also imply the candidate genes in defense pathway play an important role in resistance to MRDD in maize.
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spelling pubmed-46313342015-11-13 Genome-Wide Association Implicates Candidate Genes Conferring Resistance to Maize Rough Dwarf Disease in Maize Chen, Gengshen Wang, Xiaoming Hao, Junjie Yan, Jianbing Ding, Junqiang PLoS One Research Article Maize rough dwarf disease (MRDD) is a destructive viral disease in China, which results in 20–30% of the maize yield losses in affected areas and even as high as 100% in severely infected fields. Understanding the genetic basis of resistance will provide important insights for maize breeding program. In this study, a diverse maize population comprising of 527 inbred lines was evaluated in four environments and a genome-wide association study (GWAS) was undertaken with over 556000 SNP markers. Fifteen candidate genes associated with MRDD resistance were identified, including ten genes with annotated protein encoding functions. The homologous of nine candidate genes were predicted to relate to plant defense in different species based on published results. Significant correlation (R(2) = 0.79) between the MRDD severity and the number of resistance alleles was observed. Consequently, we have broadened the resistant germplasm to MRDD and identified a number of resistance alleles by GWAS. The results in present study also imply the candidate genes in defense pathway play an important role in resistance to MRDD in maize. Public Library of Science 2015-11-03 /pmc/articles/PMC4631334/ /pubmed/26529245 http://dx.doi.org/10.1371/journal.pone.0142001 Text en © 2015 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Chen, Gengshen
Wang, Xiaoming
Hao, Junjie
Yan, Jianbing
Ding, Junqiang
Genome-Wide Association Implicates Candidate Genes Conferring Resistance to Maize Rough Dwarf Disease in Maize
title Genome-Wide Association Implicates Candidate Genes Conferring Resistance to Maize Rough Dwarf Disease in Maize
title_full Genome-Wide Association Implicates Candidate Genes Conferring Resistance to Maize Rough Dwarf Disease in Maize
title_fullStr Genome-Wide Association Implicates Candidate Genes Conferring Resistance to Maize Rough Dwarf Disease in Maize
title_full_unstemmed Genome-Wide Association Implicates Candidate Genes Conferring Resistance to Maize Rough Dwarf Disease in Maize
title_short Genome-Wide Association Implicates Candidate Genes Conferring Resistance to Maize Rough Dwarf Disease in Maize
title_sort genome-wide association implicates candidate genes conferring resistance to maize rough dwarf disease in maize
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4631334/
https://www.ncbi.nlm.nih.gov/pubmed/26529245
http://dx.doi.org/10.1371/journal.pone.0142001
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