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The Arabidopsis a zinc finger domain protein ARS1 is essential for seed germination and ROS homeostasis in response to ABA and oxidative stress

The phytohormone abscisic acid (ABA) induces accumulation of reactive oxygen species (ROS), which can disrupt seed dormancy and plant development. Here, we report the isolation and characterization of an Arabidopsis thaliana mutant called ars1 (aba and ros sensitive 1) that showed hypersensitivity t...

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Autores principales: Baek, Dongwon, Cha, Joon-Yung, Kang, Songhwa, Park, Bokyung, Lee, Hyo-Jung, Hong, Hyewon, Chun, Hyun Jin, Kim, Doh Hoon, Kim, Min Chul, Lee, Sang Yeol, Yun, Dae-Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4631831/
https://www.ncbi.nlm.nih.gov/pubmed/26583028
http://dx.doi.org/10.3389/fpls.2015.00963
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author Baek, Dongwon
Cha, Joon-Yung
Kang, Songhwa
Park, Bokyung
Lee, Hyo-Jung
Hong, Hyewon
Chun, Hyun Jin
Kim, Doh Hoon
Kim, Min Chul
Lee, Sang Yeol
Yun, Dae-Jin
author_facet Baek, Dongwon
Cha, Joon-Yung
Kang, Songhwa
Park, Bokyung
Lee, Hyo-Jung
Hong, Hyewon
Chun, Hyun Jin
Kim, Doh Hoon
Kim, Min Chul
Lee, Sang Yeol
Yun, Dae-Jin
author_sort Baek, Dongwon
collection PubMed
description The phytohormone abscisic acid (ABA) induces accumulation of reactive oxygen species (ROS), which can disrupt seed dormancy and plant development. Here, we report the isolation and characterization of an Arabidopsis thaliana mutant called ars1 (aba and ros sensitive 1) that showed hypersensitivity to ABA during seed germination and to methyl viologen (MV) at the seedling stage. ARS1 encodes a nuclear protein with one zinc finger domain, two nuclear localization signal (NLS) domains, and one nuclear export signal (NES). The ars1 mutants showed reduced expression of a gene for superoxide dismutase (CSD3) and enhanced accumulation of ROS after ABA treatment. Transient expression of ARS1 in Arabidopsis protoplasts strongly suppressed ABA-mediated ROS production. Interestingly, nuclear-localized ARS1 translocated to the cytoplasm in response to treatment with ABA, H(2)O(2), or MV. Taken together, these results suggest that ARS1 modulates seed germination and ROS homeostasis in response to ABA and oxidative stress in plants.
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spelling pubmed-46318312015-11-18 The Arabidopsis a zinc finger domain protein ARS1 is essential for seed germination and ROS homeostasis in response to ABA and oxidative stress Baek, Dongwon Cha, Joon-Yung Kang, Songhwa Park, Bokyung Lee, Hyo-Jung Hong, Hyewon Chun, Hyun Jin Kim, Doh Hoon Kim, Min Chul Lee, Sang Yeol Yun, Dae-Jin Front Plant Sci Plant Science The phytohormone abscisic acid (ABA) induces accumulation of reactive oxygen species (ROS), which can disrupt seed dormancy and plant development. Here, we report the isolation and characterization of an Arabidopsis thaliana mutant called ars1 (aba and ros sensitive 1) that showed hypersensitivity to ABA during seed germination and to methyl viologen (MV) at the seedling stage. ARS1 encodes a nuclear protein with one zinc finger domain, two nuclear localization signal (NLS) domains, and one nuclear export signal (NES). The ars1 mutants showed reduced expression of a gene for superoxide dismutase (CSD3) and enhanced accumulation of ROS after ABA treatment. Transient expression of ARS1 in Arabidopsis protoplasts strongly suppressed ABA-mediated ROS production. Interestingly, nuclear-localized ARS1 translocated to the cytoplasm in response to treatment with ABA, H(2)O(2), or MV. Taken together, these results suggest that ARS1 modulates seed germination and ROS homeostasis in response to ABA and oxidative stress in plants. Frontiers Media S.A. 2015-11-04 /pmc/articles/PMC4631831/ /pubmed/26583028 http://dx.doi.org/10.3389/fpls.2015.00963 Text en Copyright © 2015 Baek, Cha, Kang, Park, Lee, Hong, Chun, Kim, Kim, Lee and Yun. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Baek, Dongwon
Cha, Joon-Yung
Kang, Songhwa
Park, Bokyung
Lee, Hyo-Jung
Hong, Hyewon
Chun, Hyun Jin
Kim, Doh Hoon
Kim, Min Chul
Lee, Sang Yeol
Yun, Dae-Jin
The Arabidopsis a zinc finger domain protein ARS1 is essential for seed germination and ROS homeostasis in response to ABA and oxidative stress
title The Arabidopsis a zinc finger domain protein ARS1 is essential for seed germination and ROS homeostasis in response to ABA and oxidative stress
title_full The Arabidopsis a zinc finger domain protein ARS1 is essential for seed germination and ROS homeostasis in response to ABA and oxidative stress
title_fullStr The Arabidopsis a zinc finger domain protein ARS1 is essential for seed germination and ROS homeostasis in response to ABA and oxidative stress
title_full_unstemmed The Arabidopsis a zinc finger domain protein ARS1 is essential for seed germination and ROS homeostasis in response to ABA and oxidative stress
title_short The Arabidopsis a zinc finger domain protein ARS1 is essential for seed germination and ROS homeostasis in response to ABA and oxidative stress
title_sort arabidopsis a zinc finger domain protein ars1 is essential for seed germination and ros homeostasis in response to aba and oxidative stress
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4631831/
https://www.ncbi.nlm.nih.gov/pubmed/26583028
http://dx.doi.org/10.3389/fpls.2015.00963
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