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Fine-Mapping the Wheat Snn1 Locus Conferring Sensitivity to the Parastagonospora nodorum Necrotrophic Effector SnTox1 Using an Eight Founder Multiparent Advanced Generation Inter-Cross Population

The necrotrophic fungus Parastagonospora nodorum is an important pathogen of one of the world’s most economically important cereal crops, wheat (Triticum aestivum L.). P. nodorum produces necrotrophic protein effectors that mediate host cell death, providing nutrients for continuation of the infecti...

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Autores principales: Cockram, James, Scuderi, Alice, Barber, Toby, Furuki, Eiko, Gardner, Keith A., Gosman, Nick, Kowalczyk, Radoslaw, Phan, Huyen P., Rose, Gemma A., Tan, Kar-Chun, Oliver, Richard P., Mackay, Ian J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632045/
https://www.ncbi.nlm.nih.gov/pubmed/26416667
http://dx.doi.org/10.1534/g3.115.021584
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author Cockram, James
Scuderi, Alice
Barber, Toby
Furuki, Eiko
Gardner, Keith A.
Gosman, Nick
Kowalczyk, Radoslaw
Phan, Huyen P.
Rose, Gemma A.
Tan, Kar-Chun
Oliver, Richard P.
Mackay, Ian J.
author_facet Cockram, James
Scuderi, Alice
Barber, Toby
Furuki, Eiko
Gardner, Keith A.
Gosman, Nick
Kowalczyk, Radoslaw
Phan, Huyen P.
Rose, Gemma A.
Tan, Kar-Chun
Oliver, Richard P.
Mackay, Ian J.
author_sort Cockram, James
collection PubMed
description The necrotrophic fungus Parastagonospora nodorum is an important pathogen of one of the world’s most economically important cereal crops, wheat (Triticum aestivum L.). P. nodorum produces necrotrophic protein effectors that mediate host cell death, providing nutrients for continuation of the infection process. The recent discovery of pathogen effectors has revolutionized disease resistance breeding for necrotrophic diseases in crop species, allowing often complex genetic resistance mechanisms to be broken down into constituent parts. To date, three effectors have been identified in P. nodorum. Here we use the effector, SnTox1, to screen 642 progeny from an eight-parent multiparent advanced generation inter-cross (i.e., MAGIC) population, genotyped with a 90,000-feature single-nucleotide polymorphism array. The MAGIC founders showed a range of sensitivity to SnTox1, with transgressive segregation evident in the progeny. SnTox1 sensitivity showed high heritability, with quantitative trait locus analyses fine-mapping the Snn1 locus to the short arm of chromosome 1B. In addition, a previously undescribed SnTox1 sensitivity locus was identified on the long arm of chromosome 5A, termed here QSnn.niab-5A.1. The peak single-nucleotide polymorphism for the Snn1 locus was converted to the KASP genotyping platform, providing breeders and researchers a simple and cheap diagnostic marker for allelic state at Snn1.
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spelling pubmed-46320452015-11-04 Fine-Mapping the Wheat Snn1 Locus Conferring Sensitivity to the Parastagonospora nodorum Necrotrophic Effector SnTox1 Using an Eight Founder Multiparent Advanced Generation Inter-Cross Population Cockram, James Scuderi, Alice Barber, Toby Furuki, Eiko Gardner, Keith A. Gosman, Nick Kowalczyk, Radoslaw Phan, Huyen P. Rose, Gemma A. Tan, Kar-Chun Oliver, Richard P. Mackay, Ian J. G3 (Bethesda) Multiparental Populations The necrotrophic fungus Parastagonospora nodorum is an important pathogen of one of the world’s most economically important cereal crops, wheat (Triticum aestivum L.). P. nodorum produces necrotrophic protein effectors that mediate host cell death, providing nutrients for continuation of the infection process. The recent discovery of pathogen effectors has revolutionized disease resistance breeding for necrotrophic diseases in crop species, allowing often complex genetic resistance mechanisms to be broken down into constituent parts. To date, three effectors have been identified in P. nodorum. Here we use the effector, SnTox1, to screen 642 progeny from an eight-parent multiparent advanced generation inter-cross (i.e., MAGIC) population, genotyped with a 90,000-feature single-nucleotide polymorphism array. The MAGIC founders showed a range of sensitivity to SnTox1, with transgressive segregation evident in the progeny. SnTox1 sensitivity showed high heritability, with quantitative trait locus analyses fine-mapping the Snn1 locus to the short arm of chromosome 1B. In addition, a previously undescribed SnTox1 sensitivity locus was identified on the long arm of chromosome 5A, termed here QSnn.niab-5A.1. The peak single-nucleotide polymorphism for the Snn1 locus was converted to the KASP genotyping platform, providing breeders and researchers a simple and cheap diagnostic marker for allelic state at Snn1. Genetics Society of America 2015-09-24 /pmc/articles/PMC4632045/ /pubmed/26416667 http://dx.doi.org/10.1534/g3.115.021584 Text en Copyright © 2015 Cockram et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Multiparental Populations
Cockram, James
Scuderi, Alice
Barber, Toby
Furuki, Eiko
Gardner, Keith A.
Gosman, Nick
Kowalczyk, Radoslaw
Phan, Huyen P.
Rose, Gemma A.
Tan, Kar-Chun
Oliver, Richard P.
Mackay, Ian J.
Fine-Mapping the Wheat Snn1 Locus Conferring Sensitivity to the Parastagonospora nodorum Necrotrophic Effector SnTox1 Using an Eight Founder Multiparent Advanced Generation Inter-Cross Population
title Fine-Mapping the Wheat Snn1 Locus Conferring Sensitivity to the Parastagonospora nodorum Necrotrophic Effector SnTox1 Using an Eight Founder Multiparent Advanced Generation Inter-Cross Population
title_full Fine-Mapping the Wheat Snn1 Locus Conferring Sensitivity to the Parastagonospora nodorum Necrotrophic Effector SnTox1 Using an Eight Founder Multiparent Advanced Generation Inter-Cross Population
title_fullStr Fine-Mapping the Wheat Snn1 Locus Conferring Sensitivity to the Parastagonospora nodorum Necrotrophic Effector SnTox1 Using an Eight Founder Multiparent Advanced Generation Inter-Cross Population
title_full_unstemmed Fine-Mapping the Wheat Snn1 Locus Conferring Sensitivity to the Parastagonospora nodorum Necrotrophic Effector SnTox1 Using an Eight Founder Multiparent Advanced Generation Inter-Cross Population
title_short Fine-Mapping the Wheat Snn1 Locus Conferring Sensitivity to the Parastagonospora nodorum Necrotrophic Effector SnTox1 Using an Eight Founder Multiparent Advanced Generation Inter-Cross Population
title_sort fine-mapping the wheat snn1 locus conferring sensitivity to the parastagonospora nodorum necrotrophic effector sntox1 using an eight founder multiparent advanced generation inter-cross population
topic Multiparental Populations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632045/
https://www.ncbi.nlm.nih.gov/pubmed/26416667
http://dx.doi.org/10.1534/g3.115.021584
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