Cargando…

Differential regulation of receptivity in two uterine horns of a recipient mouse following asynchronous embryo transfer

Receptivity is a limited time in which uterine endometrium can establish a successful dialogue with blastocyst. This study was to investigate the effect of asynchronous embryo transfer on uterine receptivity in mice. Embryos under different stages were transferred into two oviduct sides of a recipie...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Shi-Jie, Wang, Tong-Song, Qin, Fu-Niu, Huang, Zhu, Liang, Xiao-Huan, Gao, Fei, Song, Zhuo, Yang, Zeng-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632121/
https://www.ncbi.nlm.nih.gov/pubmed/26531680
http://dx.doi.org/10.1038/srep15897
_version_ 1782398964067205120
author Li, Shi-Jie
Wang, Tong-Song
Qin, Fu-Niu
Huang, Zhu
Liang, Xiao-Huan
Gao, Fei
Song, Zhuo
Yang, Zeng-Ming
author_facet Li, Shi-Jie
Wang, Tong-Song
Qin, Fu-Niu
Huang, Zhu
Liang, Xiao-Huan
Gao, Fei
Song, Zhuo
Yang, Zeng-Ming
author_sort Li, Shi-Jie
collection PubMed
description Receptivity is a limited time in which uterine endometrium can establish a successful dialogue with blastocyst. This study was to investigate the effect of asynchronous embryo transfer on uterine receptivity in mice. Embryos under different stages were transferred into two oviduct sides of a recipient mouse on day 1 of pseudopregnancy. Our results showed the asynchronously transferred embryos can implant in all groups. Compared to zygote-transfer group, the length of implanted embryos is longer in 8-cell embryo- or blastocyst-transfer group. The levels of Snail and COX-2 immunostaining in blastocyst-transfer group are significantly stronger than that in zygote-transfer group. Embryos in blastocyst-transfer group migrate faster than that in zygote-transfer group within uterus. Blastocysts are in a state of developmental delay after they are transferred into oviducts, and they are reactivated and implanted rapidly in uterus. The developmental rate to newborn in zygote-transfer group is obviously higher than that in blastocyst-transfer group, suggesting that a delay in embryo development and implantation will lead to a decrease of litter size. These results indicated that the window of implantation is differentially regulated in two uterine horns of a recipient by embryos at different stages.
format Online
Article
Text
id pubmed-4632121
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-46321212015-11-05 Differential regulation of receptivity in two uterine horns of a recipient mouse following asynchronous embryo transfer Li, Shi-Jie Wang, Tong-Song Qin, Fu-Niu Huang, Zhu Liang, Xiao-Huan Gao, Fei Song, Zhuo Yang, Zeng-Ming Sci Rep Article Receptivity is a limited time in which uterine endometrium can establish a successful dialogue with blastocyst. This study was to investigate the effect of asynchronous embryo transfer on uterine receptivity in mice. Embryos under different stages were transferred into two oviduct sides of a recipient mouse on day 1 of pseudopregnancy. Our results showed the asynchronously transferred embryos can implant in all groups. Compared to zygote-transfer group, the length of implanted embryos is longer in 8-cell embryo- or blastocyst-transfer group. The levels of Snail and COX-2 immunostaining in blastocyst-transfer group are significantly stronger than that in zygote-transfer group. Embryos in blastocyst-transfer group migrate faster than that in zygote-transfer group within uterus. Blastocysts are in a state of developmental delay after they are transferred into oviducts, and they are reactivated and implanted rapidly in uterus. The developmental rate to newborn in zygote-transfer group is obviously higher than that in blastocyst-transfer group, suggesting that a delay in embryo development and implantation will lead to a decrease of litter size. These results indicated that the window of implantation is differentially regulated in two uterine horns of a recipient by embryos at different stages. Nature Publishing Group 2015-11-04 /pmc/articles/PMC4632121/ /pubmed/26531680 http://dx.doi.org/10.1038/srep15897 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Li, Shi-Jie
Wang, Tong-Song
Qin, Fu-Niu
Huang, Zhu
Liang, Xiao-Huan
Gao, Fei
Song, Zhuo
Yang, Zeng-Ming
Differential regulation of receptivity in two uterine horns of a recipient mouse following asynchronous embryo transfer
title Differential regulation of receptivity in two uterine horns of a recipient mouse following asynchronous embryo transfer
title_full Differential regulation of receptivity in two uterine horns of a recipient mouse following asynchronous embryo transfer
title_fullStr Differential regulation of receptivity in two uterine horns of a recipient mouse following asynchronous embryo transfer
title_full_unstemmed Differential regulation of receptivity in two uterine horns of a recipient mouse following asynchronous embryo transfer
title_short Differential regulation of receptivity in two uterine horns of a recipient mouse following asynchronous embryo transfer
title_sort differential regulation of receptivity in two uterine horns of a recipient mouse following asynchronous embryo transfer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632121/
https://www.ncbi.nlm.nih.gov/pubmed/26531680
http://dx.doi.org/10.1038/srep15897
work_keys_str_mv AT lishijie differentialregulationofreceptivityintwouterinehornsofarecipientmousefollowingasynchronousembryotransfer
AT wangtongsong differentialregulationofreceptivityintwouterinehornsofarecipientmousefollowingasynchronousembryotransfer
AT qinfuniu differentialregulationofreceptivityintwouterinehornsofarecipientmousefollowingasynchronousembryotransfer
AT huangzhu differentialregulationofreceptivityintwouterinehornsofarecipientmousefollowingasynchronousembryotransfer
AT liangxiaohuan differentialregulationofreceptivityintwouterinehornsofarecipientmousefollowingasynchronousembryotransfer
AT gaofei differentialregulationofreceptivityintwouterinehornsofarecipientmousefollowingasynchronousembryotransfer
AT songzhuo differentialregulationofreceptivityintwouterinehornsofarecipientmousefollowingasynchronousembryotransfer
AT yangzengming differentialregulationofreceptivityintwouterinehornsofarecipientmousefollowingasynchronousembryotransfer