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Methods of high integrity RNA extraction from cell/agarose construct

BACKGROUND: Agarose hydrogels are widely used for three-dimensional cell scaffolding in tissue engineering and cell biology. Recently, molecular profiles have been obtained with extraction of a minimal volume of RNA using fluorescent-tagged quantitative polymerase chain reaction (qPCR), which requir...

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Detalles Bibliográficos
Autores principales:	Ogura, Takahiro, Tsuchiya, Akihiro, Minas, Tom, Mizuno, Shuichi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2015
Materias:	Technical Note
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632373/ https://www.ncbi.nlm.nih.gov/pubmed/26537242 http://dx.doi.org/10.1186/s13104-015-1627-5

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