Isolation and Genome Characterization of the Virulent Staphylococcus aureus Bacteriophage SA97

A novel bacteriophage that infects S. aureus, SA97, was isolated and characterized. The phage SA97 belongs to the Siphoviridae family, and the cell wall teichoic acid (WTA) was found to be a host receptor of the phage SA97. Genome analysis revealed that SA97 contains 40,592 bp of DNA encoding 54 pre...

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Autores principales: Chang, Yoonjee, Shin, Hakdong, Lee, Ju-Hoon, Park, Chul Jong, Paik, Soon-Young, Ryu, Sangryeol
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632378/
https://www.ncbi.nlm.nih.gov/pubmed/26437428
http://dx.doi.org/10.3390/v7102870
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author Chang, Yoonjee
Shin, Hakdong
Lee, Ju-Hoon
Park, Chul Jong
Paik, Soon-Young
Ryu, Sangryeol
author_facet Chang, Yoonjee
Shin, Hakdong
Lee, Ju-Hoon
Park, Chul Jong
Paik, Soon-Young
Ryu, Sangryeol
author_sort Chang, Yoonjee
collection PubMed
description A novel bacteriophage that infects S. aureus, SA97, was isolated and characterized. The phage SA97 belongs to the Siphoviridae family, and the cell wall teichoic acid (WTA) was found to be a host receptor of the phage SA97. Genome analysis revealed that SA97 contains 40,592 bp of DNA encoding 54 predicted open reading frames (ORFs), and none of these genes were related to virulence or drug resistance. Although a few genes associated with lysogen formation were detected in the phage SA97 genome, the phage SA97 produced neither lysogen nor transductant in S. aureus. These results suggest that the phage SA97 may be a promising candidate for controlling S. aureus.
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spelling pubmed-46323782015-11-23 Isolation and Genome Characterization of the Virulent Staphylococcus aureus Bacteriophage SA97 Chang, Yoonjee Shin, Hakdong Lee, Ju-Hoon Park, Chul Jong Paik, Soon-Young Ryu, Sangryeol Viruses Article A novel bacteriophage that infects S. aureus, SA97, was isolated and characterized. The phage SA97 belongs to the Siphoviridae family, and the cell wall teichoic acid (WTA) was found to be a host receptor of the phage SA97. Genome analysis revealed that SA97 contains 40,592 bp of DNA encoding 54 predicted open reading frames (ORFs), and none of these genes were related to virulence or drug resistance. Although a few genes associated with lysogen formation were detected in the phage SA97 genome, the phage SA97 produced neither lysogen nor transductant in S. aureus. These results suggest that the phage SA97 may be a promising candidate for controlling S. aureus. MDPI 2015-10-01 /pmc/articles/PMC4632378/ /pubmed/26437428 http://dx.doi.org/10.3390/v7102870 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chang, Yoonjee
Shin, Hakdong
Lee, Ju-Hoon
Park, Chul Jong
Paik, Soon-Young
Ryu, Sangryeol
Isolation and Genome Characterization of the Virulent Staphylococcus aureus Bacteriophage SA97
title Isolation and Genome Characterization of the Virulent Staphylococcus aureus Bacteriophage SA97
title_full Isolation and Genome Characterization of the Virulent Staphylococcus aureus Bacteriophage SA97
title_fullStr Isolation and Genome Characterization of the Virulent Staphylococcus aureus Bacteriophage SA97
title_full_unstemmed Isolation and Genome Characterization of the Virulent Staphylococcus aureus Bacteriophage SA97
title_short Isolation and Genome Characterization of the Virulent Staphylococcus aureus Bacteriophage SA97
title_sort isolation and genome characterization of the virulent staphylococcus aureus bacteriophage sa97
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632378/
https://www.ncbi.nlm.nih.gov/pubmed/26437428
http://dx.doi.org/10.3390/v7102870
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