Multi-Modal Imaging with a Toolbox of Influenza A Reporter Viruses

Reporter viruses are useful probes for studying multiple stages of the viral life cycle. Here we describe an expanded toolbox of fluorescent and bioluminescent influenza A reporter viruses. The enhanced utility of these tools enabled kinetic studies of viral attachment, infection, and co-infection....

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Detalles Bibliográficos
Autores principales: Tran, Vy, Poole, Daniel S., Jeffery, Justin J., Sheahan, Timothy P., Creech, Donald, Yevtodiyenko, Aleksey, Peat, Andrew J., Francis, Kevin P., You, Shihyun, Mehle, Andrew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632381/
https://www.ncbi.nlm.nih.gov/pubmed/26473913
http://dx.doi.org/10.3390/v7102873
Descripción
Sumario:Reporter viruses are useful probes for studying multiple stages of the viral life cycle. Here we describe an expanded toolbox of fluorescent and bioluminescent influenza A reporter viruses. The enhanced utility of these tools enabled kinetic studies of viral attachment, infection, and co-infection. Multi-modal bioluminescence and positron emission tomography–computed tomography (PET/CT) imaging of infected animals revealed that antiviral treatment reduced viral load, dissemination, and inflammation. These new technologies and applications will dramatically accelerate in vitro and in vivo influenza virus studies.

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