Multi-Modal Imaging with a Toolbox of Influenza A Reporter Viruses

Reporter viruses are useful probes for studying multiple stages of the viral life cycle. Here we describe an expanded toolbox of fluorescent and bioluminescent influenza A reporter viruses. The enhanced utility of these tools enabled kinetic studies of viral attachment, infection, and co-infection....

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Autores principales: Tran, Vy, Poole, Daniel S., Jeffery, Justin J., Sheahan, Timothy P., Creech, Donald, Yevtodiyenko, Aleksey, Peat, Andrew J., Francis, Kevin P., You, Shihyun, Mehle, Andrew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632381/
https://www.ncbi.nlm.nih.gov/pubmed/26473913
http://dx.doi.org/10.3390/v7102873
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author Tran, Vy
Poole, Daniel S.
Jeffery, Justin J.
Sheahan, Timothy P.
Creech, Donald
Yevtodiyenko, Aleksey
Peat, Andrew J.
Francis, Kevin P.
You, Shihyun
Mehle, Andrew
author_facet Tran, Vy
Poole, Daniel S.
Jeffery, Justin J.
Sheahan, Timothy P.
Creech, Donald
Yevtodiyenko, Aleksey
Peat, Andrew J.
Francis, Kevin P.
You, Shihyun
Mehle, Andrew
author_sort Tran, Vy
collection PubMed
description Reporter viruses are useful probes for studying multiple stages of the viral life cycle. Here we describe an expanded toolbox of fluorescent and bioluminescent influenza A reporter viruses. The enhanced utility of these tools enabled kinetic studies of viral attachment, infection, and co-infection. Multi-modal bioluminescence and positron emission tomography–computed tomography (PET/CT) imaging of infected animals revealed that antiviral treatment reduced viral load, dissemination, and inflammation. These new technologies and applications will dramatically accelerate in vitro and in vivo influenza virus studies.
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spelling pubmed-46323812015-11-23 Multi-Modal Imaging with a Toolbox of Influenza A Reporter Viruses Tran, Vy Poole, Daniel S. Jeffery, Justin J. Sheahan, Timothy P. Creech, Donald Yevtodiyenko, Aleksey Peat, Andrew J. Francis, Kevin P. You, Shihyun Mehle, Andrew Viruses Communication Reporter viruses are useful probes for studying multiple stages of the viral life cycle. Here we describe an expanded toolbox of fluorescent and bioluminescent influenza A reporter viruses. The enhanced utility of these tools enabled kinetic studies of viral attachment, infection, and co-infection. Multi-modal bioluminescence and positron emission tomography–computed tomography (PET/CT) imaging of infected animals revealed that antiviral treatment reduced viral load, dissemination, and inflammation. These new technologies and applications will dramatically accelerate in vitro and in vivo influenza virus studies. MDPI 2015-10-13 /pmc/articles/PMC4632381/ /pubmed/26473913 http://dx.doi.org/10.3390/v7102873 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Tran, Vy
Poole, Daniel S.
Jeffery, Justin J.
Sheahan, Timothy P.
Creech, Donald
Yevtodiyenko, Aleksey
Peat, Andrew J.
Francis, Kevin P.
You, Shihyun
Mehle, Andrew
Multi-Modal Imaging with a Toolbox of Influenza A Reporter Viruses
title Multi-Modal Imaging with a Toolbox of Influenza A Reporter Viruses
title_full Multi-Modal Imaging with a Toolbox of Influenza A Reporter Viruses
title_fullStr Multi-Modal Imaging with a Toolbox of Influenza A Reporter Viruses
title_full_unstemmed Multi-Modal Imaging with a Toolbox of Influenza A Reporter Viruses
title_short Multi-Modal Imaging with a Toolbox of Influenza A Reporter Viruses
title_sort multi-modal imaging with a toolbox of influenza a reporter viruses
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632381/
https://www.ncbi.nlm.nih.gov/pubmed/26473913
http://dx.doi.org/10.3390/v7102873
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