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Diagnosis of Dengue Infection Using Conventional and Biosensor Based Techniques

Dengue is an arthropod-borne viral disease caused by four antigenically different serotypes of dengue virus. This disease is considered as a major public health concern around the world. Currently, there is no licensed vaccine or antiviral drug available for the prevention and treatment of dengue di...

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Autores principales: Parkash, Om, Hanim Shueb, Rafidah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632385/
https://www.ncbi.nlm.nih.gov/pubmed/26492265
http://dx.doi.org/10.3390/v7102877
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author Parkash, Om
Hanim Shueb, Rafidah
author_facet Parkash, Om
Hanim Shueb, Rafidah
author_sort Parkash, Om
collection PubMed
description Dengue is an arthropod-borne viral disease caused by four antigenically different serotypes of dengue virus. This disease is considered as a major public health concern around the world. Currently, there is no licensed vaccine or antiviral drug available for the prevention and treatment of dengue disease. Moreover, clinical features of dengue are indistinguishable from other infectious diseases such as malaria, chikungunya, rickettsia and leptospira. Therefore, prompt and accurate laboratory diagnostic test is urgently required for disease confirmation and patient triage. The traditional diagnostic techniques for the dengue virus are viral detection in cell culture, serological testing, and RNA amplification using reverse transcriptase PCR. This paper discusses the conventional laboratory methods used for the diagnosis of dengue during the acute and convalescent phase and highlights the advantages and limitations of these routine laboratory tests. Subsequently, the biosensor based assays developed using various transducers for the detection of dengue are also reviewed.
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spelling pubmed-46323852015-11-23 Diagnosis of Dengue Infection Using Conventional and Biosensor Based Techniques Parkash, Om Hanim Shueb, Rafidah Viruses Review Dengue is an arthropod-borne viral disease caused by four antigenically different serotypes of dengue virus. This disease is considered as a major public health concern around the world. Currently, there is no licensed vaccine or antiviral drug available for the prevention and treatment of dengue disease. Moreover, clinical features of dengue are indistinguishable from other infectious diseases such as malaria, chikungunya, rickettsia and leptospira. Therefore, prompt and accurate laboratory diagnostic test is urgently required for disease confirmation and patient triage. The traditional diagnostic techniques for the dengue virus are viral detection in cell culture, serological testing, and RNA amplification using reverse transcriptase PCR. This paper discusses the conventional laboratory methods used for the diagnosis of dengue during the acute and convalescent phase and highlights the advantages and limitations of these routine laboratory tests. Subsequently, the biosensor based assays developed using various transducers for the detection of dengue are also reviewed. MDPI 2015-10-19 /pmc/articles/PMC4632385/ /pubmed/26492265 http://dx.doi.org/10.3390/v7102877 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Parkash, Om
Hanim Shueb, Rafidah
Diagnosis of Dengue Infection Using Conventional and Biosensor Based Techniques
title Diagnosis of Dengue Infection Using Conventional and Biosensor Based Techniques
title_full Diagnosis of Dengue Infection Using Conventional and Biosensor Based Techniques
title_fullStr Diagnosis of Dengue Infection Using Conventional and Biosensor Based Techniques
title_full_unstemmed Diagnosis of Dengue Infection Using Conventional and Biosensor Based Techniques
title_short Diagnosis of Dengue Infection Using Conventional and Biosensor Based Techniques
title_sort diagnosis of dengue infection using conventional and biosensor based techniques
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632385/
https://www.ncbi.nlm.nih.gov/pubmed/26492265
http://dx.doi.org/10.3390/v7102877
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