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Complete Genome and Phylogeny of Puumala Hantavirus Isolates Circulating in France

Puumala virus (PUUV) is the agent of nephropathia epidemica (NE), a mild form of hemorrhagic fever with renal syndrome (HFRS) in Europe. NE incidence presents a high spatial variation throughout France, while the geographical distribution of the wild reservoir of PUUV, the bank vole, is rather conti...

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Autores principales: Castel, Guillaume, Couteaudier, Mathilde, Sauvage, Frank, Pons, Jean-Baptiste, Murri, Séverine, Plyusnina, Angelina, Pontier, Dominique, Cosson, Jean-François, Plyusnin, Alexander, Marianneau, Philippe, Tordo, Noël
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632392/
https://www.ncbi.nlm.nih.gov/pubmed/26506370
http://dx.doi.org/10.3390/v7102884
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author Castel, Guillaume
Couteaudier, Mathilde
Sauvage, Frank
Pons, Jean-Baptiste
Murri, Séverine
Plyusnina, Angelina
Pontier, Dominique
Cosson, Jean-François
Plyusnin, Alexander
Marianneau, Philippe
Tordo, Noël
author_facet Castel, Guillaume
Couteaudier, Mathilde
Sauvage, Frank
Pons, Jean-Baptiste
Murri, Séverine
Plyusnina, Angelina
Pontier, Dominique
Cosson, Jean-François
Plyusnin, Alexander
Marianneau, Philippe
Tordo, Noël
author_sort Castel, Guillaume
collection PubMed
description Puumala virus (PUUV) is the agent of nephropathia epidemica (NE), a mild form of hemorrhagic fever with renal syndrome (HFRS) in Europe. NE incidence presents a high spatial variation throughout France, while the geographical distribution of the wild reservoir of PUUV, the bank vole, is rather continuous. A missing piece of the puzzle is the current distribution and the genetic variation of PUUV in France, which has been overlooked until now and remains poorly understood. During a population survey, from 2008 to 2011, bank voles were trapped in eight different forests of France located in areas known to be endemic for NE or in area from where no NE case has been reported until now. Bank voles were tested for immunoglobulin (Ig)G ELISA serology and two seropositive animals for each of three different areas (Ardennes, Jura and Orleans) were then subjected to laboratory analyses in order to sequence the whole S, M and L segments of PUUV. Phylogenetic analyses revealed that French PUUV isolates globally belong to the central European (CE) lineage although isolates from Ardennes are clearly distinct from those in Jura and Orleans, suggesting a different evolutionary history and origin of PUUV introduction in France. Sequence analyses revealed specific amino acid signatures along the N protein, including in PUUV from the Orleans region from where NE in humans has never been reported. The relevance of these mutations in term of pathophysiology is discussed.
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spelling pubmed-46323922015-11-23 Complete Genome and Phylogeny of Puumala Hantavirus Isolates Circulating in France Castel, Guillaume Couteaudier, Mathilde Sauvage, Frank Pons, Jean-Baptiste Murri, Séverine Plyusnina, Angelina Pontier, Dominique Cosson, Jean-François Plyusnin, Alexander Marianneau, Philippe Tordo, Noël Viruses Article Puumala virus (PUUV) is the agent of nephropathia epidemica (NE), a mild form of hemorrhagic fever with renal syndrome (HFRS) in Europe. NE incidence presents a high spatial variation throughout France, while the geographical distribution of the wild reservoir of PUUV, the bank vole, is rather continuous. A missing piece of the puzzle is the current distribution and the genetic variation of PUUV in France, which has been overlooked until now and remains poorly understood. During a population survey, from 2008 to 2011, bank voles were trapped in eight different forests of France located in areas known to be endemic for NE or in area from where no NE case has been reported until now. Bank voles were tested for immunoglobulin (Ig)G ELISA serology and two seropositive animals for each of three different areas (Ardennes, Jura and Orleans) were then subjected to laboratory analyses in order to sequence the whole S, M and L segments of PUUV. Phylogenetic analyses revealed that French PUUV isolates globally belong to the central European (CE) lineage although isolates from Ardennes are clearly distinct from those in Jura and Orleans, suggesting a different evolutionary history and origin of PUUV introduction in France. Sequence analyses revealed specific amino acid signatures along the N protein, including in PUUV from the Orleans region from where NE in humans has never been reported. The relevance of these mutations in term of pathophysiology is discussed. MDPI 2015-10-22 /pmc/articles/PMC4632392/ /pubmed/26506370 http://dx.doi.org/10.3390/v7102884 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Castel, Guillaume
Couteaudier, Mathilde
Sauvage, Frank
Pons, Jean-Baptiste
Murri, Séverine
Plyusnina, Angelina
Pontier, Dominique
Cosson, Jean-François
Plyusnin, Alexander
Marianneau, Philippe
Tordo, Noël
Complete Genome and Phylogeny of Puumala Hantavirus Isolates Circulating in France
title Complete Genome and Phylogeny of Puumala Hantavirus Isolates Circulating in France
title_full Complete Genome and Phylogeny of Puumala Hantavirus Isolates Circulating in France
title_fullStr Complete Genome and Phylogeny of Puumala Hantavirus Isolates Circulating in France
title_full_unstemmed Complete Genome and Phylogeny of Puumala Hantavirus Isolates Circulating in France
title_short Complete Genome and Phylogeny of Puumala Hantavirus Isolates Circulating in France
title_sort complete genome and phylogeny of puumala hantavirus isolates circulating in france
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632392/
https://www.ncbi.nlm.nih.gov/pubmed/26506370
http://dx.doi.org/10.3390/v7102884
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