Cargando…

Longitudinal Study of Cytokine Expression, Lipid Profile and Neuronal Growth Factors in Human Breast Milk from Term and Preterm Deliveries

Breast milk (BM) is considered as a reference for infant nutrition. The role of bioactive components, such as cytokines, hormones, growth factors (GFs) and fatty acids (FAs) is poorly known, but they might be implicated in immune response development. The aim of this study was to identify the lipid...

Descripción completa

Detalles Bibliográficos
Autores principales: Collado, Maria Carmen, Santaella, Marina, Mira-Pascual, Laia, Martínez-Arias, Elena, Khodayar-Pardo, Parisá, Ros, Gaspar, Martínez-Costa, Cecilia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632435/
https://www.ncbi.nlm.nih.gov/pubmed/26492267
http://dx.doi.org/10.3390/nu7105415
_version_ 1782399030028926976
author Collado, Maria Carmen
Santaella, Marina
Mira-Pascual, Laia
Martínez-Arias, Elena
Khodayar-Pardo, Parisá
Ros, Gaspar
Martínez-Costa, Cecilia
author_facet Collado, Maria Carmen
Santaella, Marina
Mira-Pascual, Laia
Martínez-Arias, Elena
Khodayar-Pardo, Parisá
Ros, Gaspar
Martínez-Costa, Cecilia
author_sort Collado, Maria Carmen
collection PubMed
description Breast milk (BM) is considered as a reference for infant nutrition. The role of bioactive components, such as cytokines, hormones, growth factors (GFs) and fatty acids (FAs) is poorly known, but they might be implicated in immune response development. The aim of this study was to identify the lipid profile and the spectrum of cytokines and neuronal GF in BM samples and analyse the influence of gestational age and lactation time on these components. This study used a longitudinal prospective method for the characterization of cytokines, FAs and GFs global profiles in 120 BM samples from 40 healthy mothers (20 preterm and 20 term) collected as colostrum, transitional and mature milk. The cytokines were analysed by protein array (Ray Bio® Human Cytokine Array G6. Ray Biotech, Inc. Norcross, GA, USA) and the FAs were analysed by gas chromatography. The FA profile was similar between the term and the preterm BM samples. Omega-3-α-linoleic and docosahexaenoic acid (DHA) and omega-6-linoleic acid were the most abundant in the term and preterm samples during lactation. Omega-3 ETA and omega-3 EPA we observed exclusively in the preterm samples. The cytokine profile showed a different trend based on gestational age. A significantly higher expression of neurotrophic factors was found in the mature preterm milk samples as compared to the mature term samples. Our study is the first to identify the influence and interactions of perinatal factors on cytokine, GFs and FAs in human milk.
format Online
Article
Text
id pubmed-4632435
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-46324352015-11-30 Longitudinal Study of Cytokine Expression, Lipid Profile and Neuronal Growth Factors in Human Breast Milk from Term and Preterm Deliveries Collado, Maria Carmen Santaella, Marina Mira-Pascual, Laia Martínez-Arias, Elena Khodayar-Pardo, Parisá Ros, Gaspar Martínez-Costa, Cecilia Nutrients Article Breast milk (BM) is considered as a reference for infant nutrition. The role of bioactive components, such as cytokines, hormones, growth factors (GFs) and fatty acids (FAs) is poorly known, but they might be implicated in immune response development. The aim of this study was to identify the lipid profile and the spectrum of cytokines and neuronal GF in BM samples and analyse the influence of gestational age and lactation time on these components. This study used a longitudinal prospective method for the characterization of cytokines, FAs and GFs global profiles in 120 BM samples from 40 healthy mothers (20 preterm and 20 term) collected as colostrum, transitional and mature milk. The cytokines were analysed by protein array (Ray Bio® Human Cytokine Array G6. Ray Biotech, Inc. Norcross, GA, USA) and the FAs were analysed by gas chromatography. The FA profile was similar between the term and the preterm BM samples. Omega-3-α-linoleic and docosahexaenoic acid (DHA) and omega-6-linoleic acid were the most abundant in the term and preterm samples during lactation. Omega-3 ETA and omega-3 EPA we observed exclusively in the preterm samples. The cytokine profile showed a different trend based on gestational age. A significantly higher expression of neurotrophic factors was found in the mature preterm milk samples as compared to the mature term samples. Our study is the first to identify the influence and interactions of perinatal factors on cytokine, GFs and FAs in human milk. MDPI 2015-10-19 /pmc/articles/PMC4632435/ /pubmed/26492267 http://dx.doi.org/10.3390/nu7105415 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Collado, Maria Carmen
Santaella, Marina
Mira-Pascual, Laia
Martínez-Arias, Elena
Khodayar-Pardo, Parisá
Ros, Gaspar
Martínez-Costa, Cecilia
Longitudinal Study of Cytokine Expression, Lipid Profile and Neuronal Growth Factors in Human Breast Milk from Term and Preterm Deliveries
title Longitudinal Study of Cytokine Expression, Lipid Profile and Neuronal Growth Factors in Human Breast Milk from Term and Preterm Deliveries
title_full Longitudinal Study of Cytokine Expression, Lipid Profile and Neuronal Growth Factors in Human Breast Milk from Term and Preterm Deliveries
title_fullStr Longitudinal Study of Cytokine Expression, Lipid Profile and Neuronal Growth Factors in Human Breast Milk from Term and Preterm Deliveries
title_full_unstemmed Longitudinal Study of Cytokine Expression, Lipid Profile and Neuronal Growth Factors in Human Breast Milk from Term and Preterm Deliveries
title_short Longitudinal Study of Cytokine Expression, Lipid Profile and Neuronal Growth Factors in Human Breast Milk from Term and Preterm Deliveries
title_sort longitudinal study of cytokine expression, lipid profile and neuronal growth factors in human breast milk from term and preterm deliveries
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632435/
https://www.ncbi.nlm.nih.gov/pubmed/26492267
http://dx.doi.org/10.3390/nu7105415
work_keys_str_mv AT colladomariacarmen longitudinalstudyofcytokineexpressionlipidprofileandneuronalgrowthfactorsinhumanbreastmilkfromtermandpretermdeliveries
AT santaellamarina longitudinalstudyofcytokineexpressionlipidprofileandneuronalgrowthfactorsinhumanbreastmilkfromtermandpretermdeliveries
AT mirapascuallaia longitudinalstudyofcytokineexpressionlipidprofileandneuronalgrowthfactorsinhumanbreastmilkfromtermandpretermdeliveries
AT martinezariaselena longitudinalstudyofcytokineexpressionlipidprofileandneuronalgrowthfactorsinhumanbreastmilkfromtermandpretermdeliveries
AT khodayarpardoparisa longitudinalstudyofcytokineexpressionlipidprofileandneuronalgrowthfactorsinhumanbreastmilkfromtermandpretermdeliveries
AT rosgaspar longitudinalstudyofcytokineexpressionlipidprofileandneuronalgrowthfactorsinhumanbreastmilkfromtermandpretermdeliveries
AT martinezcostacecilia longitudinalstudyofcytokineexpressionlipidprofileandneuronalgrowthfactorsinhumanbreastmilkfromtermandpretermdeliveries