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Biochar-amended potting medium reduces the susceptibility of rice to root-knot nematode infections

BACKGROUND: Biochar is a solid coproduct of biomass pyrolysis, and soil amended with biochar has been shown to enhance the productivity of various crops and induce systemic plant resistance to fungal pathogens. The aim of this study was to explore the ability of wood biochar to induce resistance to...

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Autores principales: Huang, Wen-kun, Ji, Hong-li, Gheysen, Godelieve, Debode, Jane, Kyndt, Tina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632470/
https://www.ncbi.nlm.nih.gov/pubmed/26537003
http://dx.doi.org/10.1186/s12870-015-0654-7
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author Huang, Wen-kun
Ji, Hong-li
Gheysen, Godelieve
Debode, Jane
Kyndt, Tina
author_facet Huang, Wen-kun
Ji, Hong-li
Gheysen, Godelieve
Debode, Jane
Kyndt, Tina
author_sort Huang, Wen-kun
collection PubMed
description BACKGROUND: Biochar is a solid coproduct of biomass pyrolysis, and soil amended with biochar has been shown to enhance the productivity of various crops and induce systemic plant resistance to fungal pathogens. The aim of this study was to explore the ability of wood biochar to induce resistance to the root-knot nematode (RKN) Meloidogyne graminicola in rice (Oryza sativa cv. Nipponbare) and examine its histochemical and molecular impact on plant defense mechanisms. RESULTS: A 1.2 % concentration of biochar added to the potting medium of rice was found to be the most effective at reducing nematode development in rice roots, whereas direct toxic effects of biochar exudates on nematode viability, infectivity or development were not observed. The increased plant resistance was associated with biochar-primed H(2)O(2) accumulation as well as with the transcriptional enhancement of genes involved in the ethylene (ET) signaling pathway. The increased susceptibility of the Ein2b-RNAi line, which is deficient in ET signaling, further confirmed that biochar-induced priming acts at least partly through ET signaling. CONCLUSION: These results suggest that biochar amendments protect rice plants challenged by nematodes. This priming effect partially depends on the ET signaling pathway and enhanced H(2)O(2) accumulation.
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spelling pubmed-46324702015-11-05 Biochar-amended potting medium reduces the susceptibility of rice to root-knot nematode infections Huang, Wen-kun Ji, Hong-li Gheysen, Godelieve Debode, Jane Kyndt, Tina BMC Plant Biol Research Article BACKGROUND: Biochar is a solid coproduct of biomass pyrolysis, and soil amended with biochar has been shown to enhance the productivity of various crops and induce systemic plant resistance to fungal pathogens. The aim of this study was to explore the ability of wood biochar to induce resistance to the root-knot nematode (RKN) Meloidogyne graminicola in rice (Oryza sativa cv. Nipponbare) and examine its histochemical and molecular impact on plant defense mechanisms. RESULTS: A 1.2 % concentration of biochar added to the potting medium of rice was found to be the most effective at reducing nematode development in rice roots, whereas direct toxic effects of biochar exudates on nematode viability, infectivity or development were not observed. The increased plant resistance was associated with biochar-primed H(2)O(2) accumulation as well as with the transcriptional enhancement of genes involved in the ethylene (ET) signaling pathway. The increased susceptibility of the Ein2b-RNAi line, which is deficient in ET signaling, further confirmed that biochar-induced priming acts at least partly through ET signaling. CONCLUSION: These results suggest that biochar amendments protect rice plants challenged by nematodes. This priming effect partially depends on the ET signaling pathway and enhanced H(2)O(2) accumulation. BioMed Central 2015-11-04 /pmc/articles/PMC4632470/ /pubmed/26537003 http://dx.doi.org/10.1186/s12870-015-0654-7 Text en © Huang et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Huang, Wen-kun
Ji, Hong-li
Gheysen, Godelieve
Debode, Jane
Kyndt, Tina
Biochar-amended potting medium reduces the susceptibility of rice to root-knot nematode infections
title Biochar-amended potting medium reduces the susceptibility of rice to root-knot nematode infections
title_full Biochar-amended potting medium reduces the susceptibility of rice to root-knot nematode infections
title_fullStr Biochar-amended potting medium reduces the susceptibility of rice to root-knot nematode infections
title_full_unstemmed Biochar-amended potting medium reduces the susceptibility of rice to root-knot nematode infections
title_short Biochar-amended potting medium reduces the susceptibility of rice to root-knot nematode infections
title_sort biochar-amended potting medium reduces the susceptibility of rice to root-knot nematode infections
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632470/
https://www.ncbi.nlm.nih.gov/pubmed/26537003
http://dx.doi.org/10.1186/s12870-015-0654-7
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