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Module-based construction of plasmids for chromosomal integration of the fission yeast Schizosaccharomyces pombe
Integration of an external gene into a fission yeast chromosome is useful to investigate the effect of the gene product. An easy way to knock-in a gene construct is use of an integration plasmid, which can be targeted and inserted to a chromosome through homologous recombination. Despite the advanta...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
The Royal Society
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632507/ https://www.ncbi.nlm.nih.gov/pubmed/26108218 http://dx.doi.org/10.1098/rsob.150054 |