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MyoD Is a Novel Activator of Porcine FIT1 Gene by Interacting with the Canonical E-Box Element during Myogenesis

Fat-induced transcript 1 (FIT1/FITM1) gene is a member of the conserved gene family important for triglyceride-rich lipid droplet accumulation. FIT1 gene displays a similar muscle-specific expression across pigs, mice, and humans. Thus pigs can act as a useful model of many human diseases resulting...

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Autores principales: Yan, Chi, Xia, Xiaoliang, He, Junxian, Ren, Zhuqing, Xu, Dequan, Xiong, Yuanzhu, Zuo, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632787/
https://www.ncbi.nlm.nih.gov/pubmed/26492245
http://dx.doi.org/10.3390/ijms161025014
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author Yan, Chi
Xia, Xiaoliang
He, Junxian
Ren, Zhuqing
Xu, Dequan
Xiong, Yuanzhu
Zuo, Bo
author_facet Yan, Chi
Xia, Xiaoliang
He, Junxian
Ren, Zhuqing
Xu, Dequan
Xiong, Yuanzhu
Zuo, Bo
author_sort Yan, Chi
collection PubMed
description Fat-induced transcript 1 (FIT1/FITM1) gene is a member of the conserved gene family important for triglyceride-rich lipid droplet accumulation. FIT1 gene displays a similar muscle-specific expression across pigs, mice, and humans. Thus pigs can act as a useful model of many human diseases resulting from misexpression of FIT1 gene. Triglyceride content in skeletal muscle plays a key role in pork meat quality and flavors. An insertion/deletion mutation in porcine FIT1 coding region shows a high correlation with a series of fat traits. To gain better knowledge of the potential role of FIT1 gene in human diseases and the correlations with pork meat quality, our attention is given to the region upstream of the porcine FIT1 coding sequence. We cloned ~1 kb of the 5′-flanking region of porcine FIT1 gene to define the role of this sequence in modulating the myogenic expression. A canonical E-box element that activated porcine FIT1 promoter activity during myogenesis was identified. Further analysis demonstrated that promoter activity was induced by overexpression of MyoD1, which bound to this canonical E-box during C2C12 differentiation. This is the first evidence that FIT1 as the direct novel target of MyoD is involved in muscle development.
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spelling pubmed-46327872015-11-23 MyoD Is a Novel Activator of Porcine FIT1 Gene by Interacting with the Canonical E-Box Element during Myogenesis Yan, Chi Xia, Xiaoliang He, Junxian Ren, Zhuqing Xu, Dequan Xiong, Yuanzhu Zuo, Bo Int J Mol Sci Article Fat-induced transcript 1 (FIT1/FITM1) gene is a member of the conserved gene family important for triglyceride-rich lipid droplet accumulation. FIT1 gene displays a similar muscle-specific expression across pigs, mice, and humans. Thus pigs can act as a useful model of many human diseases resulting from misexpression of FIT1 gene. Triglyceride content in skeletal muscle plays a key role in pork meat quality and flavors. An insertion/deletion mutation in porcine FIT1 coding region shows a high correlation with a series of fat traits. To gain better knowledge of the potential role of FIT1 gene in human diseases and the correlations with pork meat quality, our attention is given to the region upstream of the porcine FIT1 coding sequence. We cloned ~1 kb of the 5′-flanking region of porcine FIT1 gene to define the role of this sequence in modulating the myogenic expression. A canonical E-box element that activated porcine FIT1 promoter activity during myogenesis was identified. Further analysis demonstrated that promoter activity was induced by overexpression of MyoD1, which bound to this canonical E-box during C2C12 differentiation. This is the first evidence that FIT1 as the direct novel target of MyoD is involved in muscle development. MDPI 2015-10-20 /pmc/articles/PMC4632787/ /pubmed/26492245 http://dx.doi.org/10.3390/ijms161025014 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yan, Chi
Xia, Xiaoliang
He, Junxian
Ren, Zhuqing
Xu, Dequan
Xiong, Yuanzhu
Zuo, Bo
MyoD Is a Novel Activator of Porcine FIT1 Gene by Interacting with the Canonical E-Box Element during Myogenesis
title MyoD Is a Novel Activator of Porcine FIT1 Gene by Interacting with the Canonical E-Box Element during Myogenesis
title_full MyoD Is a Novel Activator of Porcine FIT1 Gene by Interacting with the Canonical E-Box Element during Myogenesis
title_fullStr MyoD Is a Novel Activator of Porcine FIT1 Gene by Interacting with the Canonical E-Box Element during Myogenesis
title_full_unstemmed MyoD Is a Novel Activator of Porcine FIT1 Gene by Interacting with the Canonical E-Box Element during Myogenesis
title_short MyoD Is a Novel Activator of Porcine FIT1 Gene by Interacting with the Canonical E-Box Element during Myogenesis
title_sort myod is a novel activator of porcine fit1 gene by interacting with the canonical e-box element during myogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632787/
https://www.ncbi.nlm.nih.gov/pubmed/26492245
http://dx.doi.org/10.3390/ijms161025014
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