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miR-134 Modulates the Proliferation of Human Cardiomyocyte Progenitor Cells by Targeting Meis2
Cardiomyocyte progenitor cells play essential roles in early heart development, which requires highly controlled cellular organization. microRNAs (miRs) are involved in various cell behaviors by post-transcriptional regulation of target genes. However, the roles of miRNAs in human cardiomyocyte prog...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632798/ https://www.ncbi.nlm.nih.gov/pubmed/26512644 http://dx.doi.org/10.3390/ijms161025199 |
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author | Wu, Ya-Han Zhao, Hong Zhou, Li-Ping Zhao, Chun-Xia Wu, Yu-Fei Zhen, Li-Xiao Li, Jun Ge, Dong-Xia Xu, Liang Lin, Li Liu, Yi Liang, Dan-Dan Chen, Yi-Han |
author_facet | Wu, Ya-Han Zhao, Hong Zhou, Li-Ping Zhao, Chun-Xia Wu, Yu-Fei Zhen, Li-Xiao Li, Jun Ge, Dong-Xia Xu, Liang Lin, Li Liu, Yi Liang, Dan-Dan Chen, Yi-Han |
author_sort | Wu, Ya-Han |
collection | PubMed |
description | Cardiomyocyte progenitor cells play essential roles in early heart development, which requires highly controlled cellular organization. microRNAs (miRs) are involved in various cell behaviors by post-transcriptional regulation of target genes. However, the roles of miRNAs in human cardiomyocyte progenitor cells (hCMPCs) remain to be elucidated. Our previous study showed that miR-134 was significantly downregulated in heart tissue suffering from congenital heart disease, underlying the potential role of miR-134 in cardiogenesis. In the present work, we showed that the upregulation of miR-134 reduced the proliferation of hCMPCs, as determined by EdU assay and Ki-67 immunostaining, while the inhibition of miR-134 exhibited an opposite effect. Both up- and downregulation of miR-134 expression altered the transcriptional level of cell-cycle genes. We identified Meis2 as the target of miR-134 in the regulation of hCMPC proliferation through bioinformatic prediction, luciferase reporter assay and western blot. The over-expression of Meis2 mitigated the effect of miR-134 on hCMPC proliferation. Moreover, miR-134 did not change the degree of hCMPC differentiation into cardiomyocytes in our model, suggesting that miR-134 is not required in this process. These findings reveal an essential role for miR-134 in cardiomyocyte progenitor cell biology and provide new insights into the physiology and pathology of cardiogenesis. |
format | Online Article Text |
id | pubmed-4632798 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-46327982015-11-23 miR-134 Modulates the Proliferation of Human Cardiomyocyte Progenitor Cells by Targeting Meis2 Wu, Ya-Han Zhao, Hong Zhou, Li-Ping Zhao, Chun-Xia Wu, Yu-Fei Zhen, Li-Xiao Li, Jun Ge, Dong-Xia Xu, Liang Lin, Li Liu, Yi Liang, Dan-Dan Chen, Yi-Han Int J Mol Sci Article Cardiomyocyte progenitor cells play essential roles in early heart development, which requires highly controlled cellular organization. microRNAs (miRs) are involved in various cell behaviors by post-transcriptional regulation of target genes. However, the roles of miRNAs in human cardiomyocyte progenitor cells (hCMPCs) remain to be elucidated. Our previous study showed that miR-134 was significantly downregulated in heart tissue suffering from congenital heart disease, underlying the potential role of miR-134 in cardiogenesis. In the present work, we showed that the upregulation of miR-134 reduced the proliferation of hCMPCs, as determined by EdU assay and Ki-67 immunostaining, while the inhibition of miR-134 exhibited an opposite effect. Both up- and downregulation of miR-134 expression altered the transcriptional level of cell-cycle genes. We identified Meis2 as the target of miR-134 in the regulation of hCMPC proliferation through bioinformatic prediction, luciferase reporter assay and western blot. The over-expression of Meis2 mitigated the effect of miR-134 on hCMPC proliferation. Moreover, miR-134 did not change the degree of hCMPC differentiation into cardiomyocytes in our model, suggesting that miR-134 is not required in this process. These findings reveal an essential role for miR-134 in cardiomyocyte progenitor cell biology and provide new insights into the physiology and pathology of cardiogenesis. MDPI 2015-10-23 /pmc/articles/PMC4632798/ /pubmed/26512644 http://dx.doi.org/10.3390/ijms161025199 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wu, Ya-Han Zhao, Hong Zhou, Li-Ping Zhao, Chun-Xia Wu, Yu-Fei Zhen, Li-Xiao Li, Jun Ge, Dong-Xia Xu, Liang Lin, Li Liu, Yi Liang, Dan-Dan Chen, Yi-Han miR-134 Modulates the Proliferation of Human Cardiomyocyte Progenitor Cells by Targeting Meis2 |
title | miR-134 Modulates the Proliferation of Human Cardiomyocyte Progenitor Cells by Targeting Meis2 |
title_full | miR-134 Modulates the Proliferation of Human Cardiomyocyte Progenitor Cells by Targeting Meis2 |
title_fullStr | miR-134 Modulates the Proliferation of Human Cardiomyocyte Progenitor Cells by Targeting Meis2 |
title_full_unstemmed | miR-134 Modulates the Proliferation of Human Cardiomyocyte Progenitor Cells by Targeting Meis2 |
title_short | miR-134 Modulates the Proliferation of Human Cardiomyocyte Progenitor Cells by Targeting Meis2 |
title_sort | mir-134 modulates the proliferation of human cardiomyocyte progenitor cells by targeting meis2 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632798/ https://www.ncbi.nlm.nih.gov/pubmed/26512644 http://dx.doi.org/10.3390/ijms161025199 |
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