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Acute aerobic exercise increases exogenously infused bone marrow cell retention in the heart

Stem cell therapy for myocardial infarction (MI) has been shown to improve cardiac function and reduce infarct size. Exercise training, in the form of cardiac rehabilitation, is an essential part of patient care post-MI. Hence, we tested the effects of acute and chronic aerobic exercise on stem cell...

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Autores principales: Chirico, Erica N, Ding, Dennis, Muthukumaran, Geetha, Houser, Steven R, Starosta, Tim, Mu, Anbin, Margulies, Kenneth B, Libonati, Joseph R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632949/
https://www.ncbi.nlm.nih.gov/pubmed/26486160
http://dx.doi.org/10.14814/phy2.12566
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author Chirico, Erica N
Ding, Dennis
Muthukumaran, Geetha
Houser, Steven R
Starosta, Tim
Mu, Anbin
Margulies, Kenneth B
Libonati, Joseph R
author_facet Chirico, Erica N
Ding, Dennis
Muthukumaran, Geetha
Houser, Steven R
Starosta, Tim
Mu, Anbin
Margulies, Kenneth B
Libonati, Joseph R
author_sort Chirico, Erica N
collection PubMed
description Stem cell therapy for myocardial infarction (MI) has been shown to improve cardiac function and reduce infarct size. Exercise training, in the form of cardiac rehabilitation, is an essential part of patient care post-MI. Hence, we tested the effects of acute and chronic aerobic exercise on stem cell retention and cardiac remodeling post-MI. Small epicardial MI’s were induced in 12-month-old C57BL/6 mice via cryoinjury. Two weeks post-MI, vehicle infusion (N = 4) or GFP(+) bone marrow-derived cells (BMC) were injected (tail vein I.V.) immediately after acute exercise (N = 14) or sedentary conditions (N = 14). A subset of mice continued a 5-week intervention of chronic treadmill exercise (10–13 m/min; 45 min/day; 4 days/week; N = 7) or remained sedentary (N = 6). Exercise tolerance was assessed using a graded exercise test, and cardiac function was assessed with echocardiography. Acute exercise increased GFP(+) BMC retention in the infarcted zone of the heart by 30% versus sedentary (P < 0.05). This was not associated with alterations in myocardial function or gene expression of key cell adhesion molecules. Animals treated with chronic exercise increased exercise capacity (P < 0.05) and cardiac mass (P < 0.05) without change in left ventricular ejection fraction (LVEF), infarct size, or regional wall thickness (P = NS) compared with sedentary. While BMC’s alone did not affect exercise capacity, they increased LVEF (P < 0.05) and Ki67(+) nuclei number in the border zone of the heart (P < 0.05), which was potentiated with chronic exercise training (P < 0.05). We conclude that acute exercise increases BMC retention in infarcted hearts and chronic training increases exogenous BMC-mediated effects on stimulating the cardiomyocyte cell cycle. These preclinical results suggest that exercise may help to optimize stem cell therapeutics following MI.
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spelling pubmed-46329492015-11-09 Acute aerobic exercise increases exogenously infused bone marrow cell retention in the heart Chirico, Erica N Ding, Dennis Muthukumaran, Geetha Houser, Steven R Starosta, Tim Mu, Anbin Margulies, Kenneth B Libonati, Joseph R Physiol Rep Original Research Stem cell therapy for myocardial infarction (MI) has been shown to improve cardiac function and reduce infarct size. Exercise training, in the form of cardiac rehabilitation, is an essential part of patient care post-MI. Hence, we tested the effects of acute and chronic aerobic exercise on stem cell retention and cardiac remodeling post-MI. Small epicardial MI’s were induced in 12-month-old C57BL/6 mice via cryoinjury. Two weeks post-MI, vehicle infusion (N = 4) or GFP(+) bone marrow-derived cells (BMC) were injected (tail vein I.V.) immediately after acute exercise (N = 14) or sedentary conditions (N = 14). A subset of mice continued a 5-week intervention of chronic treadmill exercise (10–13 m/min; 45 min/day; 4 days/week; N = 7) or remained sedentary (N = 6). Exercise tolerance was assessed using a graded exercise test, and cardiac function was assessed with echocardiography. Acute exercise increased GFP(+) BMC retention in the infarcted zone of the heart by 30% versus sedentary (P < 0.05). This was not associated with alterations in myocardial function or gene expression of key cell adhesion molecules. Animals treated with chronic exercise increased exercise capacity (P < 0.05) and cardiac mass (P < 0.05) without change in left ventricular ejection fraction (LVEF), infarct size, or regional wall thickness (P = NS) compared with sedentary. While BMC’s alone did not affect exercise capacity, they increased LVEF (P < 0.05) and Ki67(+) nuclei number in the border zone of the heart (P < 0.05), which was potentiated with chronic exercise training (P < 0.05). We conclude that acute exercise increases BMC retention in infarcted hearts and chronic training increases exogenous BMC-mediated effects on stimulating the cardiomyocyte cell cycle. These preclinical results suggest that exercise may help to optimize stem cell therapeutics following MI. John Wiley & Sons, Ltd 2015-10-20 /pmc/articles/PMC4632949/ /pubmed/26486160 http://dx.doi.org/10.14814/phy2.12566 Text en © 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Chirico, Erica N
Ding, Dennis
Muthukumaran, Geetha
Houser, Steven R
Starosta, Tim
Mu, Anbin
Margulies, Kenneth B
Libonati, Joseph R
Acute aerobic exercise increases exogenously infused bone marrow cell retention in the heart
title Acute aerobic exercise increases exogenously infused bone marrow cell retention in the heart
title_full Acute aerobic exercise increases exogenously infused bone marrow cell retention in the heart
title_fullStr Acute aerobic exercise increases exogenously infused bone marrow cell retention in the heart
title_full_unstemmed Acute aerobic exercise increases exogenously infused bone marrow cell retention in the heart
title_short Acute aerobic exercise increases exogenously infused bone marrow cell retention in the heart
title_sort acute aerobic exercise increases exogenously infused bone marrow cell retention in the heart
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4632949/
https://www.ncbi.nlm.nih.gov/pubmed/26486160
http://dx.doi.org/10.14814/phy2.12566
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