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Carboranyl-Chlorin e(6) as a Potent Antimicrobial Photosensitizer

Antimicrobial photodynamic inactivation is currently being widely considered as alternative to antibiotic chemotherapy of infective diseases, attracting much attention to design of novel effective photosensitizers. Carboranyl-chlorin-e(6) (the conjugate of chlorin e(6) with carborane), applied here...

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Detalles Bibliográficos
Autores principales: Omarova, Elena O., Nazarov, Pavel A., Firsov, Alexander M., Strakhovskaya, Marina G., Arkhipova, Anastasia Yu., Moisenovich, Mikhail M., Agapov, Igor I., Ol’shevskaya, Valentina A., Zaitsev, Andrey V., Kalinin, Valery N., Kotova, Elena A., Antonenko, Yuri N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4633095/
https://www.ncbi.nlm.nih.gov/pubmed/26535905
http://dx.doi.org/10.1371/journal.pone.0141990
Descripción
Sumario:Antimicrobial photodynamic inactivation is currently being widely considered as alternative to antibiotic chemotherapy of infective diseases, attracting much attention to design of novel effective photosensitizers. Carboranyl-chlorin-e(6) (the conjugate of chlorin e(6) with carborane), applied here for the first time for antimicrobial photodynamic inactivation, appeared to be much stronger than chlorin e(6) against Gram-positive bacteria, such as Bacillus subtilis, Staphyllococcus aureus and Mycobacterium sp. Confocal fluorescence spectroscopy and membrane leakage experiments indicated that bacteria cell death upon photodynamic treatment with carboranyl-chlorin-e(6) is caused by loss of cell membrane integrity. The enhanced photobactericidal activity was attributed to the increased accumulation of the conjugate by bacterial cells, as evaluated both by centrifugation and fluorescence correlation spectroscopy. Gram-negative bacteria were rather resistant to antimicrobial photodynamic inactivation mediated by carboranyl-chlorin-e(6). Unlike chlorin e(6), the conjugate showed higher (compared to the wild-type strain) dark toxicity with Escherichia coli ΔtolC mutant, deficient in TolC-requiring multidrug efflux transporters.