Squid ink polysaccharide reduces cyclophosphamide-induced testicular damage via Nrf2/ARE activation pathway in mice

OBJECTIVE(S): Cyclophosphamide (CP) toxicity on testis was hampered by squid ink polysaccharide (SIP) via restoration of antioxidant ability in our previous investigations. This study investigated roles of Nrf2/ARE signal pathway in testis of treated mice. MATERIALS AND METHODS: Male Kunming mice we...

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Autores principales: Le, Xiaoyan, Luo, Ping, Gu, Yipeng, Tao, Yexing, Liu, Huazhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2015
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4633467/
https://www.ncbi.nlm.nih.gov/pubmed/26557973
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author Le, Xiaoyan
Luo, Ping
Gu, Yipeng
Tao, Yexing
Liu, Huazhong
author_facet Le, Xiaoyan
Luo, Ping
Gu, Yipeng
Tao, Yexing
Liu, Huazhong
author_sort Le, Xiaoyan
collection PubMed
description OBJECTIVE(S): Cyclophosphamide (CP) toxicity on testis was hampered by squid ink polysaccharide (SIP) via restoration of antioxidant ability in our previous investigations. This study investigated roles of Nrf2/ARE signal pathway in testis of treated mice. MATERIALS AND METHODS: Male Kunming mice were employed to undergo treatment with SIP and/or CP. Protein levels of Nrf2, keap-1, histone deacetylase 2 (HDAC2), quinone oxidoreductase 1 (NQO-1), and heme oxygenase 1 (HO-1) and phosphorylation level of protein kinase C (PKC) in testis were evaluated by Western blotting. RESULTS: Data showed that SIP elevated expressions of NQO-1 and HO-1 genes, two downstream target molecules of Nrf2, via activating Nrf2 to play preventive roles on CP-treated testis, and further discovered that upstream regulators of Nrf2, keap-1, HDAC2, and PKC, were concerned with the regulation of Nrf2. CONCLUSION: These results suggest that SIP could effectively weaken CP-associated testicular damage via Nrf2/ARE signal pathway.
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spelling pubmed-46334672015-11-10 Squid ink polysaccharide reduces cyclophosphamide-induced testicular damage via Nrf2/ARE activation pathway in mice Le, Xiaoyan Luo, Ping Gu, Yipeng Tao, Yexing Liu, Huazhong Iran J Basic Med Sci Short Communication OBJECTIVE(S): Cyclophosphamide (CP) toxicity on testis was hampered by squid ink polysaccharide (SIP) via restoration of antioxidant ability in our previous investigations. This study investigated roles of Nrf2/ARE signal pathway in testis of treated mice. MATERIALS AND METHODS: Male Kunming mice were employed to undergo treatment with SIP and/or CP. Protein levels of Nrf2, keap-1, histone deacetylase 2 (HDAC2), quinone oxidoreductase 1 (NQO-1), and heme oxygenase 1 (HO-1) and phosphorylation level of protein kinase C (PKC) in testis were evaluated by Western blotting. RESULTS: Data showed that SIP elevated expressions of NQO-1 and HO-1 genes, two downstream target molecules of Nrf2, via activating Nrf2 to play preventive roles on CP-treated testis, and further discovered that upstream regulators of Nrf2, keap-1, HDAC2, and PKC, were concerned with the regulation of Nrf2. CONCLUSION: These results suggest that SIP could effectively weaken CP-associated testicular damage via Nrf2/ARE signal pathway. Mashhad University of Medical Sciences 2015-08 /pmc/articles/PMC4633467/ /pubmed/26557973 Text en Copyright: © Iranian Journal of Basic Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Le, Xiaoyan
Luo, Ping
Gu, Yipeng
Tao, Yexing
Liu, Huazhong
Squid ink polysaccharide reduces cyclophosphamide-induced testicular damage via Nrf2/ARE activation pathway in mice
title Squid ink polysaccharide reduces cyclophosphamide-induced testicular damage via Nrf2/ARE activation pathway in mice
title_full Squid ink polysaccharide reduces cyclophosphamide-induced testicular damage via Nrf2/ARE activation pathway in mice
title_fullStr Squid ink polysaccharide reduces cyclophosphamide-induced testicular damage via Nrf2/ARE activation pathway in mice
title_full_unstemmed Squid ink polysaccharide reduces cyclophosphamide-induced testicular damage via Nrf2/ARE activation pathway in mice
title_short Squid ink polysaccharide reduces cyclophosphamide-induced testicular damage via Nrf2/ARE activation pathway in mice
title_sort squid ink polysaccharide reduces cyclophosphamide-induced testicular damage via nrf2/are activation pathway in mice
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4633467/
https://www.ncbi.nlm.nih.gov/pubmed/26557973
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