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Manual control of catalytic reactions: Reactions by an apoenzyme gel and a cofactor gel
Enzymes play a vital role in catalysing almost all chemical reactions that occur in biological systems. Some enzymes must form complexes with non-protein molecules called cofactors to express catalytic activities. Although the control of catalytic reactions via apoenzyme–cofactor complexes has attra...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4633677/ https://www.ncbi.nlm.nih.gov/pubmed/26537172 http://dx.doi.org/10.1038/srep16254 |
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author | Kobayashi, Yuichiro Takashima, Yoshinori Hashidzume, Akihito Yamaguchi, Hiroyasu Harada, Akira |
author_facet | Kobayashi, Yuichiro Takashima, Yoshinori Hashidzume, Akihito Yamaguchi, Hiroyasu Harada, Akira |
author_sort | Kobayashi, Yuichiro |
collection | PubMed |
description | Enzymes play a vital role in catalysing almost all chemical reactions that occur in biological systems. Some enzymes must form complexes with non-protein molecules called cofactors to express catalytic activities. Although the control of catalytic reactions via apoenzyme–cofactor complexes has attracted significant attention, the reports have been limited to the microscale. Here, we report a system to express catalytic activity by adhesion of an apoenzyme gel and a cofactor gel. The apoenzyme and cofactor gels act as catalysts when they form a gel assembly, but they lose catalytic ability upon manual dissociation. We successfully construct a system with switchable catalytic activity via adhesion and separation of the apoenzyme gel with the cofactor gel. We expect that this methodology can be applied to regulate the functional activities of enzymes that bear cofactors in their active sites, such as the oxygen transport of haemoglobin or myoglobin and the electron transport of cytochromes. |
format | Online Article Text |
id | pubmed-4633677 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-46336772015-11-25 Manual control of catalytic reactions: Reactions by an apoenzyme gel and a cofactor gel Kobayashi, Yuichiro Takashima, Yoshinori Hashidzume, Akihito Yamaguchi, Hiroyasu Harada, Akira Sci Rep Article Enzymes play a vital role in catalysing almost all chemical reactions that occur in biological systems. Some enzymes must form complexes with non-protein molecules called cofactors to express catalytic activities. Although the control of catalytic reactions via apoenzyme–cofactor complexes has attracted significant attention, the reports have been limited to the microscale. Here, we report a system to express catalytic activity by adhesion of an apoenzyme gel and a cofactor gel. The apoenzyme and cofactor gels act as catalysts when they form a gel assembly, but they lose catalytic ability upon manual dissociation. We successfully construct a system with switchable catalytic activity via adhesion and separation of the apoenzyme gel with the cofactor gel. We expect that this methodology can be applied to regulate the functional activities of enzymes that bear cofactors in their active sites, such as the oxygen transport of haemoglobin or myoglobin and the electron transport of cytochromes. Nature Publishing Group 2015-11-05 /pmc/articles/PMC4633677/ /pubmed/26537172 http://dx.doi.org/10.1038/srep16254 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Kobayashi, Yuichiro Takashima, Yoshinori Hashidzume, Akihito Yamaguchi, Hiroyasu Harada, Akira Manual control of catalytic reactions: Reactions by an apoenzyme gel and a cofactor gel |
title | Manual control of catalytic reactions: Reactions by an apoenzyme gel and a cofactor gel |
title_full | Manual control of catalytic reactions: Reactions by an apoenzyme gel and a cofactor gel |
title_fullStr | Manual control of catalytic reactions: Reactions by an apoenzyme gel and a cofactor gel |
title_full_unstemmed | Manual control of catalytic reactions: Reactions by an apoenzyme gel and a cofactor gel |
title_short | Manual control of catalytic reactions: Reactions by an apoenzyme gel and a cofactor gel |
title_sort | manual control of catalytic reactions: reactions by an apoenzyme gel and a cofactor gel |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4633677/ https://www.ncbi.nlm.nih.gov/pubmed/26537172 http://dx.doi.org/10.1038/srep16254 |
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