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Characterization of neuraminidase inhibitor‐resistant influenza A(H1N1)pdm09 viruses isolated in four seasons during pandemic and post‐pandemic periods in Japan

BACKGROUND/OBJECTIVES: Japan has the highest frequency of neuraminidase (NA) inhibitor use against influenza in the world. Therefore, Japan could be at high risk of the emergence and spread of NA inhibitor‐resistant viruses. The aim of this study was to monitor the emergence of NA inhibitor‐resistan...

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Autores principales: Takashita, Emi, Fujisaki, Seiichiro, Kishida, Noriko, Xu, Hong, Imai, Masaki, Tashiro, Masato, Odagiri, Takato
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4634248/
https://www.ncbi.nlm.nih.gov/pubmed/23745712
http://dx.doi.org/10.1111/irv.12132
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author Takashita, Emi
Fujisaki, Seiichiro
Kishida, Noriko
Xu, Hong
Imai, Masaki
Tashiro, Masato
Odagiri, Takato
author_facet Takashita, Emi
Fujisaki, Seiichiro
Kishida, Noriko
Xu, Hong
Imai, Masaki
Tashiro, Masato
Odagiri, Takato
author_sort Takashita, Emi
collection PubMed
description BACKGROUND/OBJECTIVES: Japan has the highest frequency of neuraminidase (NA) inhibitor use against influenza in the world. Therefore, Japan could be at high risk of the emergence and spread of NA inhibitor‐resistant viruses. The aim of this study was to monitor the emergence of NA inhibitor‐resistant viruses and the possibility of human‐to‐human transmission during four influenza seasons in Japan. METHODS: To monitor antiviral‐resistant A(H1N1)pdm09 viruses, we examined viruses isolated in four seasons from the 2008–2009 season through the 2011–2012 season in Japan by allelic discrimination, NA gene sequencing, and NA inhibitor susceptibility. RESULTS: We found that 157 (1·3%) of 12 026 A(H1N1)pdm09 isolates possessed an H275Y substitution in the NA protein that confers about 400‐ and 140‐fold decreased susceptibility to oseltamivir and peramivir, respectively, compared with 275H wild‐type viruses. The detection rate of resistant viruses increased from 1·0% during the pandemic period to 2·0% during the post‐pandemic period. The highest detection rate of the resistant viruses was found in patients who were 0–9 years old. Furthermore, among the cases with resistant viruses, the percentage of no known exposure to antiviral drugs increased from 16% during the pandemic period to 44% during the post‐pandemic period, implying that suspected human‐to‐human transmission of the resistant viruses gradually increased in the post‐pandemic period. CONCLUSIONS: A(H1N1)pdm09 viruses resistant to oseltamivir and peramivir were sporadically detected in Japan, but they did not spread throughout the community. No viruses resistant to zanamivir and laninamivir were detected.
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spelling pubmed-46342482015-12-01 Characterization of neuraminidase inhibitor‐resistant influenza A(H1N1)pdm09 viruses isolated in four seasons during pandemic and post‐pandemic periods in Japan Takashita, Emi Fujisaki, Seiichiro Kishida, Noriko Xu, Hong Imai, Masaki Tashiro, Masato Odagiri, Takato Influenza Other Respir Viruses Part 5 BACKGROUND/OBJECTIVES: Japan has the highest frequency of neuraminidase (NA) inhibitor use against influenza in the world. Therefore, Japan could be at high risk of the emergence and spread of NA inhibitor‐resistant viruses. The aim of this study was to monitor the emergence of NA inhibitor‐resistant viruses and the possibility of human‐to‐human transmission during four influenza seasons in Japan. METHODS: To monitor antiviral‐resistant A(H1N1)pdm09 viruses, we examined viruses isolated in four seasons from the 2008–2009 season through the 2011–2012 season in Japan by allelic discrimination, NA gene sequencing, and NA inhibitor susceptibility. RESULTS: We found that 157 (1·3%) of 12 026 A(H1N1)pdm09 isolates possessed an H275Y substitution in the NA protein that confers about 400‐ and 140‐fold decreased susceptibility to oseltamivir and peramivir, respectively, compared with 275H wild‐type viruses. The detection rate of resistant viruses increased from 1·0% during the pandemic period to 2·0% during the post‐pandemic period. The highest detection rate of the resistant viruses was found in patients who were 0–9 years old. Furthermore, among the cases with resistant viruses, the percentage of no known exposure to antiviral drugs increased from 16% during the pandemic period to 44% during the post‐pandemic period, implying that suspected human‐to‐human transmission of the resistant viruses gradually increased in the post‐pandemic period. CONCLUSIONS: A(H1N1)pdm09 viruses resistant to oseltamivir and peramivir were sporadically detected in Japan, but they did not spread throughout the community. No viruses resistant to zanamivir and laninamivir were detected. John Wiley and Sons Inc. 2013-06-08 2013-11 /pmc/articles/PMC4634248/ /pubmed/23745712 http://dx.doi.org/10.1111/irv.12132 Text en © 2013 John Wiley & Sons Ltd
spellingShingle Part 5
Takashita, Emi
Fujisaki, Seiichiro
Kishida, Noriko
Xu, Hong
Imai, Masaki
Tashiro, Masato
Odagiri, Takato
Characterization of neuraminidase inhibitor‐resistant influenza A(H1N1)pdm09 viruses isolated in four seasons during pandemic and post‐pandemic periods in Japan
title Characterization of neuraminidase inhibitor‐resistant influenza A(H1N1)pdm09 viruses isolated in four seasons during pandemic and post‐pandemic periods in Japan
title_full Characterization of neuraminidase inhibitor‐resistant influenza A(H1N1)pdm09 viruses isolated in four seasons during pandemic and post‐pandemic periods in Japan
title_fullStr Characterization of neuraminidase inhibitor‐resistant influenza A(H1N1)pdm09 viruses isolated in four seasons during pandemic and post‐pandemic periods in Japan
title_full_unstemmed Characterization of neuraminidase inhibitor‐resistant influenza A(H1N1)pdm09 viruses isolated in four seasons during pandemic and post‐pandemic periods in Japan
title_short Characterization of neuraminidase inhibitor‐resistant influenza A(H1N1)pdm09 viruses isolated in four seasons during pandemic and post‐pandemic periods in Japan
title_sort characterization of neuraminidase inhibitor‐resistant influenza a(h1n1)pdm09 viruses isolated in four seasons during pandemic and post‐pandemic periods in japan
topic Part 5
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4634248/
https://www.ncbi.nlm.nih.gov/pubmed/23745712
http://dx.doi.org/10.1111/irv.12132
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