Cargando…
Feasibility and accuracy evaluation of three human papillomavirus assays for FTA card-based sampling: a pilot study in cervical cancer screening
BACKGROUND: Liquid-state specimen carriers are inadequate for sample transportation in large-scale screening projects in low-resource settings, which necessitates the exploration of novel non-hazardous solid-state alternatives. Studies investigating the feasibility and accuracy of a solid-state huma...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4634578/ https://www.ncbi.nlm.nih.gov/pubmed/26537356 http://dx.doi.org/10.1186/s12885-015-1882-9 |
_version_ | 1782399382493069312 |
---|---|
author | Wang, Shao-Ming Hu, Shang-Ying Chen, Wen Chen, Feng Zhao, Fang-Hui He, Wei Ma, Xin-Ming Zhang, Yu-Qing Wang, Jian Sivasubramaniam, Priya Qiao, You-Lin |
author_facet | Wang, Shao-Ming Hu, Shang-Ying Chen, Wen Chen, Feng Zhao, Fang-Hui He, Wei Ma, Xin-Ming Zhang, Yu-Qing Wang, Jian Sivasubramaniam, Priya Qiao, You-Lin |
author_sort | Wang, Shao-Ming |
collection | PubMed |
description | BACKGROUND: Liquid-state specimen carriers are inadequate for sample transportation in large-scale screening projects in low-resource settings, which necessitates the exploration of novel non-hazardous solid-state alternatives. Studies investigating the feasibility and accuracy of a solid-state human papillomavirus (HPV) sampling medium in combination with different down-stream HPV DNA assays for cervical cancer screening are needed. METHODS: We collected two cervical specimens from 396 women, aged 25–65 years, who were enrolled in a cervical cancer screening trial. One sample was stored using DCM preservative solution and the other was applied to a Whatman Indicating FTA Elute® card (FTA card). All specimens were processed using three HPV testing methods, including Hybrid capture 2 (HC2), careHPV™, and Cobas®4800 tests. All the women underwent a rigorous colposcopic evaluation that included using a microbiopsy protocol. RESULTS: Compared to the liquid-based carrier, the FTA card demonstrated comparable sensitivity for detecting high grade Cervical Intraepithelial Neoplasia (CIN) using HC2 (91.7 %), careHPV™ (83.3 %), and Cobas®4800 (91.7 %) tests. Moreover, the FTA card showed a higher specificity compared to a liquid-based carrier for HC2 (79.5 % vs. 71.6 %, P = 0.015), comparable specificity for careHPV™ (78.1 % vs. 73.0 %, P > 0.05), but lower specificity for the Cobas®4800 test (62.4 % vs. 69.9 %, P = 0.032). Generally, the FTA card-based sampling medium’s accuracy was comparable with that of liquid-based medium for the three HPV testing assays. CONCLUSIONS: FTA cards are a promising sample carrier for cervical cancer screening. With further optimization, it can be utilized for HPV testing in areas of varying economic development. |
format | Online Article Text |
id | pubmed-4634578 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-46345782015-11-06 Feasibility and accuracy evaluation of three human papillomavirus assays for FTA card-based sampling: a pilot study in cervical cancer screening Wang, Shao-Ming Hu, Shang-Ying Chen, Wen Chen, Feng Zhao, Fang-Hui He, Wei Ma, Xin-Ming Zhang, Yu-Qing Wang, Jian Sivasubramaniam, Priya Qiao, You-Lin BMC Cancer Research Article BACKGROUND: Liquid-state specimen carriers are inadequate for sample transportation in large-scale screening projects in low-resource settings, which necessitates the exploration of novel non-hazardous solid-state alternatives. Studies investigating the feasibility and accuracy of a solid-state human papillomavirus (HPV) sampling medium in combination with different down-stream HPV DNA assays for cervical cancer screening are needed. METHODS: We collected two cervical specimens from 396 women, aged 25–65 years, who were enrolled in a cervical cancer screening trial. One sample was stored using DCM preservative solution and the other was applied to a Whatman Indicating FTA Elute® card (FTA card). All specimens were processed using three HPV testing methods, including Hybrid capture 2 (HC2), careHPV™, and Cobas®4800 tests. All the women underwent a rigorous colposcopic evaluation that included using a microbiopsy protocol. RESULTS: Compared to the liquid-based carrier, the FTA card demonstrated comparable sensitivity for detecting high grade Cervical Intraepithelial Neoplasia (CIN) using HC2 (91.7 %), careHPV™ (83.3 %), and Cobas®4800 (91.7 %) tests. Moreover, the FTA card showed a higher specificity compared to a liquid-based carrier for HC2 (79.5 % vs. 71.6 %, P = 0.015), comparable specificity for careHPV™ (78.1 % vs. 73.0 %, P > 0.05), but lower specificity for the Cobas®4800 test (62.4 % vs. 69.9 %, P = 0.032). Generally, the FTA card-based sampling medium’s accuracy was comparable with that of liquid-based medium for the three HPV testing assays. CONCLUSIONS: FTA cards are a promising sample carrier for cervical cancer screening. With further optimization, it can be utilized for HPV testing in areas of varying economic development. BioMed Central 2015-11-04 /pmc/articles/PMC4634578/ /pubmed/26537356 http://dx.doi.org/10.1186/s12885-015-1882-9 Text en © Wang et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Wang, Shao-Ming Hu, Shang-Ying Chen, Wen Chen, Feng Zhao, Fang-Hui He, Wei Ma, Xin-Ming Zhang, Yu-Qing Wang, Jian Sivasubramaniam, Priya Qiao, You-Lin Feasibility and accuracy evaluation of three human papillomavirus assays for FTA card-based sampling: a pilot study in cervical cancer screening |
title | Feasibility and accuracy evaluation of three human papillomavirus assays for FTA card-based sampling: a pilot study in cervical cancer screening |
title_full | Feasibility and accuracy evaluation of three human papillomavirus assays for FTA card-based sampling: a pilot study in cervical cancer screening |
title_fullStr | Feasibility and accuracy evaluation of three human papillomavirus assays for FTA card-based sampling: a pilot study in cervical cancer screening |
title_full_unstemmed | Feasibility and accuracy evaluation of three human papillomavirus assays for FTA card-based sampling: a pilot study in cervical cancer screening |
title_short | Feasibility and accuracy evaluation of three human papillomavirus assays for FTA card-based sampling: a pilot study in cervical cancer screening |
title_sort | feasibility and accuracy evaluation of three human papillomavirus assays for fta card-based sampling: a pilot study in cervical cancer screening |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4634578/ https://www.ncbi.nlm.nih.gov/pubmed/26537356 http://dx.doi.org/10.1186/s12885-015-1882-9 |
work_keys_str_mv | AT wangshaoming feasibilityandaccuracyevaluationofthreehumanpapillomavirusassaysforftacardbasedsamplingapilotstudyincervicalcancerscreening AT hushangying feasibilityandaccuracyevaluationofthreehumanpapillomavirusassaysforftacardbasedsamplingapilotstudyincervicalcancerscreening AT chenwen feasibilityandaccuracyevaluationofthreehumanpapillomavirusassaysforftacardbasedsamplingapilotstudyincervicalcancerscreening AT chenfeng feasibilityandaccuracyevaluationofthreehumanpapillomavirusassaysforftacardbasedsamplingapilotstudyincervicalcancerscreening AT zhaofanghui feasibilityandaccuracyevaluationofthreehumanpapillomavirusassaysforftacardbasedsamplingapilotstudyincervicalcancerscreening AT hewei feasibilityandaccuracyevaluationofthreehumanpapillomavirusassaysforftacardbasedsamplingapilotstudyincervicalcancerscreening AT maxinming feasibilityandaccuracyevaluationofthreehumanpapillomavirusassaysforftacardbasedsamplingapilotstudyincervicalcancerscreening AT zhangyuqing feasibilityandaccuracyevaluationofthreehumanpapillomavirusassaysforftacardbasedsamplingapilotstudyincervicalcancerscreening AT wangjian feasibilityandaccuracyevaluationofthreehumanpapillomavirusassaysforftacardbasedsamplingapilotstudyincervicalcancerscreening AT sivasubramaniampriya feasibilityandaccuracyevaluationofthreehumanpapillomavirusassaysforftacardbasedsamplingapilotstudyincervicalcancerscreening AT qiaoyoulin feasibilityandaccuracyevaluationofthreehumanpapillomavirusassaysforftacardbasedsamplingapilotstudyincervicalcancerscreening |