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Performance of influenza rapid point‐of‐care tests in the detection of swine lineage A(H1N1) influenza viruses

Background  In April 2009, an A(H1N1) influenza virus of swine lineage was detected in humans in the USA, and in just over a month has infected over 10 000 people in more than 40 countries. Objectives  To determine the performance of the Binax Now, BD Directigen EZ, and the Quidel QuickVue influenza...

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Autores principales: Hurt, Aeron C., Baas, Chantal, Deng, Yi‐Mo, Roberts, Sally, Kelso, Anne, Barr, Ian G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4634687/
https://www.ncbi.nlm.nih.gov/pubmed/19627374
http://dx.doi.org/10.1111/j.1750-2659.2009.00086.x
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author Hurt, Aeron C.
Baas, Chantal
Deng, Yi‐Mo
Roberts, Sally
Kelso, Anne
Barr, Ian G.
author_facet Hurt, Aeron C.
Baas, Chantal
Deng, Yi‐Mo
Roberts, Sally
Kelso, Anne
Barr, Ian G.
author_sort Hurt, Aeron C.
collection PubMed
description Background  In April 2009, an A(H1N1) influenza virus of swine lineage was detected in humans in the USA, and in just over a month has infected over 10 000 people in more than 40 countries. Objectives  To determine the performance of the Binax Now, BD Directigen EZ, and the Quidel QuickVue influenza rapid point‐of‐care (POC) tests for the detection of the recently emerged swine lineage A(H1N1) virus. Methods  Swine lineage A(H1N1) and human seasonal influenza strains were cultured and then diluted to specific infectivity titres. Viral dilutions were assayed by the rapid POC tests and by real‐time RT‐PCR. Results  All three of the rapid POC tests successfully detected the swine lineage A(H1N1) viruses at levels between 10(3) and 10(5) TCID(50)/ml (tissue culture infectious dose(50)), with the BD Directigen test demonstrating marginally greater sensitivity than the other two tests. Viral infectivity and RNA load data for viruses at the detection limit of the rapid test kits, suggested that both the Quidel and the Binax tests were less sensitive for the detection of swine lineage A(H1N1) viruses than for human seasonal strains. In comparison the BD Directigen demonstrated similar sensitivity when detecting swine lineage A(H1N1) and human seasonal viruses. Conclusions  The three rapid POC tests all detected the emergent swine lineage A(H1N1) virus when it was present at high virus concentrations. Early diagnosis of infection can assist in the rapid treatment. However the tests are significantly less sensitive than PCR assays and as such, negative results should be verified by a laboratory test.
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spelling pubmed-46346872015-11-27 Performance of influenza rapid point‐of‐care tests in the detection of swine lineage A(H1N1) influenza viruses Hurt, Aeron C. Baas, Chantal Deng, Yi‐Mo Roberts, Sally Kelso, Anne Barr, Ian G. Influenza Other Respir Viruses Original Articles Background  In April 2009, an A(H1N1) influenza virus of swine lineage was detected in humans in the USA, and in just over a month has infected over 10 000 people in more than 40 countries. Objectives  To determine the performance of the Binax Now, BD Directigen EZ, and the Quidel QuickVue influenza rapid point‐of‐care (POC) tests for the detection of the recently emerged swine lineage A(H1N1) virus. Methods  Swine lineage A(H1N1) and human seasonal influenza strains were cultured and then diluted to specific infectivity titres. Viral dilutions were assayed by the rapid POC tests and by real‐time RT‐PCR. Results  All three of the rapid POC tests successfully detected the swine lineage A(H1N1) viruses at levels between 10(3) and 10(5) TCID(50)/ml (tissue culture infectious dose(50)), with the BD Directigen test demonstrating marginally greater sensitivity than the other two tests. Viral infectivity and RNA load data for viruses at the detection limit of the rapid test kits, suggested that both the Quidel and the Binax tests were less sensitive for the detection of swine lineage A(H1N1) viruses than for human seasonal strains. In comparison the BD Directigen demonstrated similar sensitivity when detecting swine lineage A(H1N1) and human seasonal viruses. Conclusions  The three rapid POC tests all detected the emergent swine lineage A(H1N1) virus when it was present at high virus concentrations. Early diagnosis of infection can assist in the rapid treatment. However the tests are significantly less sensitive than PCR assays and as such, negative results should be verified by a laboratory test. Blackwell Publishing Ltd 2009-06-01 2009-07 /pmc/articles/PMC4634687/ /pubmed/19627374 http://dx.doi.org/10.1111/j.1750-2659.2009.00086.x Text en © 2009 Blackwell Publishing Ltd
spellingShingle Original Articles
Hurt, Aeron C.
Baas, Chantal
Deng, Yi‐Mo
Roberts, Sally
Kelso, Anne
Barr, Ian G.
Performance of influenza rapid point‐of‐care tests in the detection of swine lineage A(H1N1) influenza viruses
title Performance of influenza rapid point‐of‐care tests in the detection of swine lineage A(H1N1) influenza viruses
title_full Performance of influenza rapid point‐of‐care tests in the detection of swine lineage A(H1N1) influenza viruses
title_fullStr Performance of influenza rapid point‐of‐care tests in the detection of swine lineage A(H1N1) influenza viruses
title_full_unstemmed Performance of influenza rapid point‐of‐care tests in the detection of swine lineage A(H1N1) influenza viruses
title_short Performance of influenza rapid point‐of‐care tests in the detection of swine lineage A(H1N1) influenza viruses
title_sort performance of influenza rapid point‐of‐care tests in the detection of swine lineage a(h1n1) influenza viruses
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4634687/
https://www.ncbi.nlm.nih.gov/pubmed/19627374
http://dx.doi.org/10.1111/j.1750-2659.2009.00086.x
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