Neutralization of Japanese Encephalitis Virus by heme-induced broadly reactive human monoclonal antibody

Geographical expansion and re-emerging new genotypes of the Japanese encephalitis virus (JEV) require the development of novel therapeutic approaches. Here, we studied a non-conventional approach for antibody therapy and show that, upon exposure to heme, a fraction of natural human immunoglobulins a...

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Autores principales: Gupta, Nimesh, de Wispelaere, Mélissanne, Lecerf, Maxime, Kalia, Manjula, Scheel, Tobias, Vrati, Sudhanshu, Berek, Claudia, Kaveri, Srinivas V., Desprès, Philippe, Lacroix-Desmazes, Sébastien, Dimitrov, Jordan D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4635365/
https://www.ncbi.nlm.nih.gov/pubmed/26542535
http://dx.doi.org/10.1038/srep16248
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author Gupta, Nimesh
de Wispelaere, Mélissanne
Lecerf, Maxime
Kalia, Manjula
Scheel, Tobias
Vrati, Sudhanshu
Berek, Claudia
Kaveri, Srinivas V.
Desprès, Philippe
Lacroix-Desmazes, Sébastien
Dimitrov, Jordan D.
author_facet Gupta, Nimesh
de Wispelaere, Mélissanne
Lecerf, Maxime
Kalia, Manjula
Scheel, Tobias
Vrati, Sudhanshu
Berek, Claudia
Kaveri, Srinivas V.
Desprès, Philippe
Lacroix-Desmazes, Sébastien
Dimitrov, Jordan D.
author_sort Gupta, Nimesh
collection PubMed
description Geographical expansion and re-emerging new genotypes of the Japanese encephalitis virus (JEV) require the development of novel therapeutic approaches. Here, we studied a non-conventional approach for antibody therapy and show that, upon exposure to heme, a fraction of natural human immunoglobulins acquires high-affinity reactivity with the antigenic domain-III of JEV E glycoprotein. These JEV-reactive antibodies exhibited neutralizing activity against recently dominant JEV genotypes. This study opens new therapeutic options for Japanese encephalitis.
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spelling pubmed-46353652015-11-25 Neutralization of Japanese Encephalitis Virus by heme-induced broadly reactive human monoclonal antibody Gupta, Nimesh de Wispelaere, Mélissanne Lecerf, Maxime Kalia, Manjula Scheel, Tobias Vrati, Sudhanshu Berek, Claudia Kaveri, Srinivas V. Desprès, Philippe Lacroix-Desmazes, Sébastien Dimitrov, Jordan D. Sci Rep Article Geographical expansion and re-emerging new genotypes of the Japanese encephalitis virus (JEV) require the development of novel therapeutic approaches. Here, we studied a non-conventional approach for antibody therapy and show that, upon exposure to heme, a fraction of natural human immunoglobulins acquires high-affinity reactivity with the antigenic domain-III of JEV E glycoprotein. These JEV-reactive antibodies exhibited neutralizing activity against recently dominant JEV genotypes. This study opens new therapeutic options for Japanese encephalitis. Nature Publishing Group 2015-11-06 /pmc/articles/PMC4635365/ /pubmed/26542535 http://dx.doi.org/10.1038/srep16248 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Gupta, Nimesh
de Wispelaere, Mélissanne
Lecerf, Maxime
Kalia, Manjula
Scheel, Tobias
Vrati, Sudhanshu
Berek, Claudia
Kaveri, Srinivas V.
Desprès, Philippe
Lacroix-Desmazes, Sébastien
Dimitrov, Jordan D.
Neutralization of Japanese Encephalitis Virus by heme-induced broadly reactive human monoclonal antibody
title Neutralization of Japanese Encephalitis Virus by heme-induced broadly reactive human monoclonal antibody
title_full Neutralization of Japanese Encephalitis Virus by heme-induced broadly reactive human monoclonal antibody
title_fullStr Neutralization of Japanese Encephalitis Virus by heme-induced broadly reactive human monoclonal antibody
title_full_unstemmed Neutralization of Japanese Encephalitis Virus by heme-induced broadly reactive human monoclonal antibody
title_short Neutralization of Japanese Encephalitis Virus by heme-induced broadly reactive human monoclonal antibody
title_sort neutralization of japanese encephalitis virus by heme-induced broadly reactive human monoclonal antibody
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4635365/
https://www.ncbi.nlm.nih.gov/pubmed/26542535
http://dx.doi.org/10.1038/srep16248
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