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Permissivity of primary hepatocytes and hepatoma cell lines to support hepatitis C virus infection
The major cell type supporting hepatitis C virus (HCV) infection is the hepatocyte; however, most reports studying viral entry and replication utilize transformed hepatoma cell lines. We demonstrate that HCV pseudoparticles (HCVpp) infect primary hepatocytes with comparable rates to hepatoma cells,...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society for General Microbiology
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4635487/ https://www.ncbi.nlm.nih.gov/pubmed/25667327 http://dx.doi.org/10.1099/vir.0.000085 |
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author | Wilson, Garrick K. Farquhar, Michelle J. Meredith, Luke Dhawan, Anil Mitry, Ragai Balfe, Peter McKeating, Jane A. |
author_facet | Wilson, Garrick K. Farquhar, Michelle J. Meredith, Luke Dhawan, Anil Mitry, Ragai Balfe, Peter McKeating, Jane A. |
author_sort | Wilson, Garrick K. |
collection | PubMed |
description | The major cell type supporting hepatitis C virus (HCV) infection is the hepatocyte; however, most reports studying viral entry and replication utilize transformed hepatoma cell lines. We demonstrate that HCV pseudoparticles (HCVpp) infect primary hepatocytes with comparable rates to hepatoma cells, demonstrating the limited variability in donor hepatocytes to support HCV receptor-glycoprotein-dependent entry. In contrast, we observed a 2-log range in viral replication between the same donor hepatocytes. We noted that cell proliferation augments pseudoparticle reporter activity and arresting hepatoma cells yields comparable levels of infection to hepatocytes. This study demonstrates comparable rates of HCVpp entry into primary hepatocytes and hepatoma cells, validating the use of transformed cells as a model system to study HCV entry. |
format | Online Article Text |
id | pubmed-4635487 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Society for General Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-46354872015-12-15 Permissivity of primary hepatocytes and hepatoma cell lines to support hepatitis C virus infection Wilson, Garrick K. Farquhar, Michelle J. Meredith, Luke Dhawan, Anil Mitry, Ragai Balfe, Peter McKeating, Jane A. J Gen Virol Animal The major cell type supporting hepatitis C virus (HCV) infection is the hepatocyte; however, most reports studying viral entry and replication utilize transformed hepatoma cell lines. We demonstrate that HCV pseudoparticles (HCVpp) infect primary hepatocytes with comparable rates to hepatoma cells, demonstrating the limited variability in donor hepatocytes to support HCV receptor-glycoprotein-dependent entry. In contrast, we observed a 2-log range in viral replication between the same donor hepatocytes. We noted that cell proliferation augments pseudoparticle reporter activity and arresting hepatoma cells yields comparable levels of infection to hepatocytes. This study demonstrates comparable rates of HCVpp entry into primary hepatocytes and hepatoma cells, validating the use of transformed cells as a model system to study HCV entry. Society for General Microbiology 2015-06 /pmc/articles/PMC4635487/ /pubmed/25667327 http://dx.doi.org/10.1099/vir.0.000085 Text en © 2015 The Authors http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Animal Wilson, Garrick K. Farquhar, Michelle J. Meredith, Luke Dhawan, Anil Mitry, Ragai Balfe, Peter McKeating, Jane A. Permissivity of primary hepatocytes and hepatoma cell lines to support hepatitis C virus infection |
title | Permissivity of primary hepatocytes and hepatoma cell lines to support hepatitis C virus infection |
title_full | Permissivity of primary hepatocytes and hepatoma cell lines to support hepatitis C virus infection |
title_fullStr | Permissivity of primary hepatocytes and hepatoma cell lines to support hepatitis C virus infection |
title_full_unstemmed | Permissivity of primary hepatocytes and hepatoma cell lines to support hepatitis C virus infection |
title_short | Permissivity of primary hepatocytes and hepatoma cell lines to support hepatitis C virus infection |
title_sort | permissivity of primary hepatocytes and hepatoma cell lines to support hepatitis c virus infection |
topic | Animal |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4635487/ https://www.ncbi.nlm.nih.gov/pubmed/25667327 http://dx.doi.org/10.1099/vir.0.000085 |
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