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Permissivity of primary hepatocytes and hepatoma cell lines to support hepatitis C virus infection

The major cell type supporting hepatitis C virus (HCV) infection is the hepatocyte; however, most reports studying viral entry and replication utilize transformed hepatoma cell lines. We demonstrate that HCV pseudoparticles (HCVpp) infect primary hepatocytes with comparable rates to hepatoma cells,...

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Autores principales: Wilson, Garrick K., Farquhar, Michelle J., Meredith, Luke, Dhawan, Anil, Mitry, Ragai, Balfe, Peter, McKeating, Jane A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society for General Microbiology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4635487/
https://www.ncbi.nlm.nih.gov/pubmed/25667327
http://dx.doi.org/10.1099/vir.0.000085
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author Wilson, Garrick K.
Farquhar, Michelle J.
Meredith, Luke
Dhawan, Anil
Mitry, Ragai
Balfe, Peter
McKeating, Jane A.
author_facet Wilson, Garrick K.
Farquhar, Michelle J.
Meredith, Luke
Dhawan, Anil
Mitry, Ragai
Balfe, Peter
McKeating, Jane A.
author_sort Wilson, Garrick K.
collection PubMed
description The major cell type supporting hepatitis C virus (HCV) infection is the hepatocyte; however, most reports studying viral entry and replication utilize transformed hepatoma cell lines. We demonstrate that HCV pseudoparticles (HCVpp) infect primary hepatocytes with comparable rates to hepatoma cells, demonstrating the limited variability in donor hepatocytes to support HCV receptor-glycoprotein-dependent entry. In contrast, we observed a 2-log range in viral replication between the same donor hepatocytes. We noted that cell proliferation augments pseudoparticle reporter activity and arresting hepatoma cells yields comparable levels of infection to hepatocytes. This study demonstrates comparable rates of HCVpp entry into primary hepatocytes and hepatoma cells, validating the use of transformed cells as a model system to study HCV entry.
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spelling pubmed-46354872015-12-15 Permissivity of primary hepatocytes and hepatoma cell lines to support hepatitis C virus infection Wilson, Garrick K. Farquhar, Michelle J. Meredith, Luke Dhawan, Anil Mitry, Ragai Balfe, Peter McKeating, Jane A. J Gen Virol Animal The major cell type supporting hepatitis C virus (HCV) infection is the hepatocyte; however, most reports studying viral entry and replication utilize transformed hepatoma cell lines. We demonstrate that HCV pseudoparticles (HCVpp) infect primary hepatocytes with comparable rates to hepatoma cells, demonstrating the limited variability in donor hepatocytes to support HCV receptor-glycoprotein-dependent entry. In contrast, we observed a 2-log range in viral replication between the same donor hepatocytes. We noted that cell proliferation augments pseudoparticle reporter activity and arresting hepatoma cells yields comparable levels of infection to hepatocytes. This study demonstrates comparable rates of HCVpp entry into primary hepatocytes and hepatoma cells, validating the use of transformed cells as a model system to study HCV entry. Society for General Microbiology 2015-06 /pmc/articles/PMC4635487/ /pubmed/25667327 http://dx.doi.org/10.1099/vir.0.000085 Text en © 2015 The Authors http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Animal
Wilson, Garrick K.
Farquhar, Michelle J.
Meredith, Luke
Dhawan, Anil
Mitry, Ragai
Balfe, Peter
McKeating, Jane A.
Permissivity of primary hepatocytes and hepatoma cell lines to support hepatitis C virus infection
title Permissivity of primary hepatocytes and hepatoma cell lines to support hepatitis C virus infection
title_full Permissivity of primary hepatocytes and hepatoma cell lines to support hepatitis C virus infection
title_fullStr Permissivity of primary hepatocytes and hepatoma cell lines to support hepatitis C virus infection
title_full_unstemmed Permissivity of primary hepatocytes and hepatoma cell lines to support hepatitis C virus infection
title_short Permissivity of primary hepatocytes and hepatoma cell lines to support hepatitis C virus infection
title_sort permissivity of primary hepatocytes and hepatoma cell lines to support hepatitis c virus infection
topic Animal
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4635487/
https://www.ncbi.nlm.nih.gov/pubmed/25667327
http://dx.doi.org/10.1099/vir.0.000085
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