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Dendritic and Axonal Architecture of Individual Pyramidal Neurons across Layers of Adult Human Neocortex
The size and shape of dendrites and axons are strong determinants of neuronal information processing. Our knowledge on neuronal structure and function is primarily based on brains of laboratory animals. Whether it translates to human is not known since quantitative data on “full” human neuronal morp...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4635923/ https://www.ncbi.nlm.nih.gov/pubmed/26318661 http://dx.doi.org/10.1093/cercor/bhv188 |
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author | Mohan, Hemanth Verhoog, Matthijs B. Doreswamy, Keerthi K. Eyal, Guy Aardse, Romy Lodder, Brendan N. Goriounova, Natalia A. Asamoah, Boateng B. Brakspear, A.B. Clementine Groot, Colin van der Sluis, Sophie Testa-Silva, Guilherme Obermayer, Joshua Boudewijns, Zimbo S.R.M. Narayanan, Rajeevan T. Baayen, Johannes C. Segev, Idan Mansvelder, Huibert D. de Kock, Christiaan P.J. |
author_facet | Mohan, Hemanth Verhoog, Matthijs B. Doreswamy, Keerthi K. Eyal, Guy Aardse, Romy Lodder, Brendan N. Goriounova, Natalia A. Asamoah, Boateng B. Brakspear, A.B. Clementine Groot, Colin van der Sluis, Sophie Testa-Silva, Guilherme Obermayer, Joshua Boudewijns, Zimbo S.R.M. Narayanan, Rajeevan T. Baayen, Johannes C. Segev, Idan Mansvelder, Huibert D. de Kock, Christiaan P.J. |
author_sort | Mohan, Hemanth |
collection | PubMed |
description | The size and shape of dendrites and axons are strong determinants of neuronal information processing. Our knowledge on neuronal structure and function is primarily based on brains of laboratory animals. Whether it translates to human is not known since quantitative data on “full” human neuronal morphologies are lacking. Here, we obtained human brain tissue during resection surgery and reconstructed basal and apical dendrites and axons of individual neurons across all cortical layers in temporal cortex (Brodmann area 21). Importantly, morphologies did not correlate to etiology, disease severity, or disease duration. Next, we show that human L(ayer) 2 and L3 pyramidal neurons have 3-fold larger dendritic length and increased branch complexity with longer segments compared with temporal cortex neurons from macaque and mouse. Unsupervised cluster analysis classified 88% of human L2 and L3 neurons into human-specific clusters distinct from mouse and macaque neurons. Computational modeling of passive electrical properties to assess the functional impact of large dendrites indicates stronger signal attenuation of electrical inputs compared with mouse. We thus provide a quantitative analysis of “full” human neuron morphologies and present direct evidence that human neurons are not “scaled-up” versions of rodent or macaque neurons, but have unique structural and functional properties. |
format | Online Article Text |
id | pubmed-4635923 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-46359232015-11-09 Dendritic and Axonal Architecture of Individual Pyramidal Neurons across Layers of Adult Human Neocortex Mohan, Hemanth Verhoog, Matthijs B. Doreswamy, Keerthi K. Eyal, Guy Aardse, Romy Lodder, Brendan N. Goriounova, Natalia A. Asamoah, Boateng B. Brakspear, A.B. Clementine Groot, Colin van der Sluis, Sophie Testa-Silva, Guilherme Obermayer, Joshua Boudewijns, Zimbo S.R.M. Narayanan, Rajeevan T. Baayen, Johannes C. Segev, Idan Mansvelder, Huibert D. de Kock, Christiaan P.J. Cereb Cortex Articles The size and shape of dendrites and axons are strong determinants of neuronal information processing. Our knowledge on neuronal structure and function is primarily based on brains of laboratory animals. Whether it translates to human is not known since quantitative data on “full” human neuronal morphologies are lacking. Here, we obtained human brain tissue during resection surgery and reconstructed basal and apical dendrites and axons of individual neurons across all cortical layers in temporal cortex (Brodmann area 21). Importantly, morphologies did not correlate to etiology, disease severity, or disease duration. Next, we show that human L(ayer) 2 and L3 pyramidal neurons have 3-fold larger dendritic length and increased branch complexity with longer segments compared with temporal cortex neurons from macaque and mouse. Unsupervised cluster analysis classified 88% of human L2 and L3 neurons into human-specific clusters distinct from mouse and macaque neurons. Computational modeling of passive electrical properties to assess the functional impact of large dendrites indicates stronger signal attenuation of electrical inputs compared with mouse. We thus provide a quantitative analysis of “full” human neuron morphologies and present direct evidence that human neurons are not “scaled-up” versions of rodent or macaque neurons, but have unique structural and functional properties. Oxford University Press 2015-12 2015-08-28 /pmc/articles/PMC4635923/ /pubmed/26318661 http://dx.doi.org/10.1093/cercor/bhv188 Text en © The Author 2015. Published by Oxford University Press http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Articles Mohan, Hemanth Verhoog, Matthijs B. Doreswamy, Keerthi K. Eyal, Guy Aardse, Romy Lodder, Brendan N. Goriounova, Natalia A. Asamoah, Boateng B. Brakspear, A.B. Clementine Groot, Colin van der Sluis, Sophie Testa-Silva, Guilherme Obermayer, Joshua Boudewijns, Zimbo S.R.M. Narayanan, Rajeevan T. Baayen, Johannes C. Segev, Idan Mansvelder, Huibert D. de Kock, Christiaan P.J. Dendritic and Axonal Architecture of Individual Pyramidal Neurons across Layers of Adult Human Neocortex |
title | Dendritic and Axonal Architecture of Individual Pyramidal Neurons across Layers of Adult Human Neocortex |
title_full | Dendritic and Axonal Architecture of Individual Pyramidal Neurons across Layers of Adult Human Neocortex |
title_fullStr | Dendritic and Axonal Architecture of Individual Pyramidal Neurons across Layers of Adult Human Neocortex |
title_full_unstemmed | Dendritic and Axonal Architecture of Individual Pyramidal Neurons across Layers of Adult Human Neocortex |
title_short | Dendritic and Axonal Architecture of Individual Pyramidal Neurons across Layers of Adult Human Neocortex |
title_sort | dendritic and axonal architecture of individual pyramidal neurons across layers of adult human neocortex |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4635923/ https://www.ncbi.nlm.nih.gov/pubmed/26318661 http://dx.doi.org/10.1093/cercor/bhv188 |
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