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An improved ScoreCard to assess the differentiation potential of human pluripotent stem cells
Research on human pluripotent stem cells has been hampered by the lack of a standardized, quantitative, scalable assay of pluripotency. We have previously described an assay called ScoreCard that used gene expression signatures to quantify differentiation efficiency. Here we report an improved versi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4636964/ https://www.ncbi.nlm.nih.gov/pubmed/26501952 http://dx.doi.org/10.1038/nbt.3387 |
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author | Tsankov, Alexander M. Akopian, Veronika Pop, Ramona Chetty, Sundari Gifford, Casey A. Daheron, Laurence Melton, Douglas A. Tsankova, Nadejda M. Meissner, Alexander |
author_facet | Tsankov, Alexander M. Akopian, Veronika Pop, Ramona Chetty, Sundari Gifford, Casey A. Daheron, Laurence Melton, Douglas A. Tsankova, Nadejda M. Meissner, Alexander |
author_sort | Tsankov, Alexander M. |
collection | PubMed |
description | Research on human pluripotent stem cells has been hampered by the lack of a standardized, quantitative, scalable assay of pluripotency. We have previously described an assay called ScoreCard that used gene expression signatures to quantify differentiation efficiency. Here we report an improved version of the assay based on qPCR that enables faster, more quantitative assessment of functional pluripotency. We provide an in-depth characterization of the revised signature panel through embryoid body and directed differentiation experiments as well as a detailed comparison to the teratoma assay. We also show that the improved ScoreCard enables applications such as screening of small molecules, genetic perturbations and assessment of culture conditions. Beyond stem cell applications, this approach can in principle be extended to other cell types and lineages. |
format | Online Article Text |
id | pubmed-4636964 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
record_format | MEDLINE/PubMed |
spelling | pubmed-46369642016-05-01 An improved ScoreCard to assess the differentiation potential of human pluripotent stem cells Tsankov, Alexander M. Akopian, Veronika Pop, Ramona Chetty, Sundari Gifford, Casey A. Daheron, Laurence Melton, Douglas A. Tsankova, Nadejda M. Meissner, Alexander Nat Biotechnol Article Research on human pluripotent stem cells has been hampered by the lack of a standardized, quantitative, scalable assay of pluripotency. We have previously described an assay called ScoreCard that used gene expression signatures to quantify differentiation efficiency. Here we report an improved version of the assay based on qPCR that enables faster, more quantitative assessment of functional pluripotency. We provide an in-depth characterization of the revised signature panel through embryoid body and directed differentiation experiments as well as a detailed comparison to the teratoma assay. We also show that the improved ScoreCard enables applications such as screening of small molecules, genetic perturbations and assessment of culture conditions. Beyond stem cell applications, this approach can in principle be extended to other cell types and lineages. 2015-10-26 2015-11 /pmc/articles/PMC4636964/ /pubmed/26501952 http://dx.doi.org/10.1038/nbt.3387 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Tsankov, Alexander M. Akopian, Veronika Pop, Ramona Chetty, Sundari Gifford, Casey A. Daheron, Laurence Melton, Douglas A. Tsankova, Nadejda M. Meissner, Alexander An improved ScoreCard to assess the differentiation potential of human pluripotent stem cells |
title | An improved ScoreCard to assess the differentiation potential of human pluripotent stem cells |
title_full | An improved ScoreCard to assess the differentiation potential of human pluripotent stem cells |
title_fullStr | An improved ScoreCard to assess the differentiation potential of human pluripotent stem cells |
title_full_unstemmed | An improved ScoreCard to assess the differentiation potential of human pluripotent stem cells |
title_short | An improved ScoreCard to assess the differentiation potential of human pluripotent stem cells |
title_sort | improved scorecard to assess the differentiation potential of human pluripotent stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4636964/ https://www.ncbi.nlm.nih.gov/pubmed/26501952 http://dx.doi.org/10.1038/nbt.3387 |
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