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Late progamic phase and fertilization affect calreticulin expression in the Hyacinthus orientalis female gametophyte
KEY MESSAGE: Calreticulin expression is upregulated during sexual reproduction ofHyacinthus orientalis, and the protein is localized both in the cytoplasm and a highly specialized cell wall within the female gametophyte. ABSTRACT: Several evidences indicate calreticulin (CRT) as an important calcium...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4636998/ https://www.ncbi.nlm.nih.gov/pubmed/26354004 http://dx.doi.org/10.1007/s00299-015-1863-0 |
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author | Niedojadło, Katarzyna Lenartowski, Robert Lenartowska, Marta Bednarska-Kozakiewicz, Elżbieta |
author_facet | Niedojadło, Katarzyna Lenartowski, Robert Lenartowska, Marta Bednarska-Kozakiewicz, Elżbieta |
author_sort | Niedojadło, Katarzyna |
collection | PubMed |
description | KEY MESSAGE: Calreticulin expression is upregulated during sexual reproduction ofHyacinthus orientalis, and the protein is localized both in the cytoplasm and a highly specialized cell wall within the female gametophyte. ABSTRACT: Several evidences indicate calreticulin (CRT) as an important calcium (Ca(2+))-binding protein that is involved in the generative reproduction of higher plants, including both pre-fertilization and post-fertilization events. Because CRT is able to bind and sequester exchangeable Ca(2+), it can serve as a mobile intracellular store of easily releasable Ca(2+) and control its local cytosolic concentrations in the embryo sac. This phenomenon seems to be essential during the late progamic phase, gamete fusion, and early embryogenesis. In this report, we demonstrate the differential expression of CRT within Hyacinthus female gametophyte cells before and during anthesis, during the late progamic phase when the pollen tube enters the embryo sac, and at the moment of fertilization and zygote/early endosperm activation. CRT mRNA and the protein localize mainly to the endoplasmic reticulum (ER) and Golgi compartments of the cells, which are involved in sexual reproduction events, such as those in sister synergids, the egg cell, the central cell, zygote and the developing endosperm. Additionally, immunogold research demonstrates selective CRT distribution in the filiform apparatus (FA), a highly specific component of the synergid cell wall. In the light of our previous data showing the total transcriptional activity of the Hyacinthus female gametophyte and the results presented here, we discuss the possible functions of CRT with respect to the critical role of Ca(2+) homeostasis during key events of sexual plant reproduction. Moreover, we propose that the elevated expression of CRT within the female gametophyte is a universal phenomenon in the cells involved in double fertilization in higher plants. |
format | Online Article Text |
id | pubmed-4636998 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-46369982015-11-12 Late progamic phase and fertilization affect calreticulin expression in the Hyacinthus orientalis female gametophyte Niedojadło, Katarzyna Lenartowski, Robert Lenartowska, Marta Bednarska-Kozakiewicz, Elżbieta Plant Cell Rep Original Article KEY MESSAGE: Calreticulin expression is upregulated during sexual reproduction ofHyacinthus orientalis, and the protein is localized both in the cytoplasm and a highly specialized cell wall within the female gametophyte. ABSTRACT: Several evidences indicate calreticulin (CRT) as an important calcium (Ca(2+))-binding protein that is involved in the generative reproduction of higher plants, including both pre-fertilization and post-fertilization events. Because CRT is able to bind and sequester exchangeable Ca(2+), it can serve as a mobile intracellular store of easily releasable Ca(2+) and control its local cytosolic concentrations in the embryo sac. This phenomenon seems to be essential during the late progamic phase, gamete fusion, and early embryogenesis. In this report, we demonstrate the differential expression of CRT within Hyacinthus female gametophyte cells before and during anthesis, during the late progamic phase when the pollen tube enters the embryo sac, and at the moment of fertilization and zygote/early endosperm activation. CRT mRNA and the protein localize mainly to the endoplasmic reticulum (ER) and Golgi compartments of the cells, which are involved in sexual reproduction events, such as those in sister synergids, the egg cell, the central cell, zygote and the developing endosperm. Additionally, immunogold research demonstrates selective CRT distribution in the filiform apparatus (FA), a highly specific component of the synergid cell wall. In the light of our previous data showing the total transcriptional activity of the Hyacinthus female gametophyte and the results presented here, we discuss the possible functions of CRT with respect to the critical role of Ca(2+) homeostasis during key events of sexual plant reproduction. Moreover, we propose that the elevated expression of CRT within the female gametophyte is a universal phenomenon in the cells involved in double fertilization in higher plants. Springer Berlin Heidelberg 2015-09-09 2015 /pmc/articles/PMC4636998/ /pubmed/26354004 http://dx.doi.org/10.1007/s00299-015-1863-0 Text en © The Author(s) 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Niedojadło, Katarzyna Lenartowski, Robert Lenartowska, Marta Bednarska-Kozakiewicz, Elżbieta Late progamic phase and fertilization affect calreticulin expression in the Hyacinthus orientalis female gametophyte |
title | Late progamic phase and fertilization affect calreticulin expression in the Hyacinthus orientalis female gametophyte |
title_full | Late progamic phase and fertilization affect calreticulin expression in the Hyacinthus orientalis female gametophyte |
title_fullStr | Late progamic phase and fertilization affect calreticulin expression in the Hyacinthus orientalis female gametophyte |
title_full_unstemmed | Late progamic phase and fertilization affect calreticulin expression in the Hyacinthus orientalis female gametophyte |
title_short | Late progamic phase and fertilization affect calreticulin expression in the Hyacinthus orientalis female gametophyte |
title_sort | late progamic phase and fertilization affect calreticulin expression in the hyacinthus orientalis female gametophyte |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4636998/ https://www.ncbi.nlm.nih.gov/pubmed/26354004 http://dx.doi.org/10.1007/s00299-015-1863-0 |
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