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Seminal plasma hexosaminidase in patients with normal and abnormal spermograms

BACKGROUND: Glycosidases profusion in male reproductive fluids suggests a possible relationship with sperm function. Although Hexosaminidase (Hex) is the most active glycosidase in epididymal fluid and seminal plasma, as well as in spermatozoa, Glucosidase is considered a marker for epididymal funct...

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Autores principales: Menendez-Helman, Renata Julia, Sanjurjo, Claudia, Miranda, Patricia Vivian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research and Clinical Center for Infertility 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4637121/
https://www.ncbi.nlm.nih.gov/pubmed/26568758
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author Menendez-Helman, Renata Julia
Sanjurjo, Claudia
Miranda, Patricia Vivian
author_facet Menendez-Helman, Renata Julia
Sanjurjo, Claudia
Miranda, Patricia Vivian
author_sort Menendez-Helman, Renata Julia
collection PubMed
description BACKGROUND: Glycosidases profusion in male reproductive fluids suggests a possible relationship with sperm function. Although Hexosaminidase (Hex) is the most active glycosidase in epididymal fluid and seminal plasma, as well as in spermatozoa, Glucosidase is considered a marker for epididymal function and azoospermia. OBJECTIVE: The aim of this study was to determine Hex activity in seminal plasma from patients with normal and abnormal spermograms and analyze its correlation with seminal parameters. MATERIALS AND METHODS: In this cross sectional study, seminal plasma from azoospermic, asthenozoospermic, teratozoospermic, and normozoospermic patients was analyzed for the activity of: total Hex, HexA isoform, and glucosidase. Besides, hexosamine levels were determined, and the amount of Hex was quantified by immunoblot with a specific antibody. Correlation of Hex activity with seminal parameters was also analyzed. RESULTS: Hex activity, like glucosidase, was significantly reduced in azoospermic samples (44, 49, and 60% reduction for total Hex, HexA and glucosidase, respectively). A reduced amount of Hex in azoospermic samples was confirmed by western immunoblot. Hex activity was negatively correlated with round cells in azoospermic samples and positively correlated with motility in asthenozoospermic ones. CONCLUSION: The results suggested that Hex activity was reduced in azoospermic samples and this was due to a lower amount of enzyme. The correlation to seminal parameters related to particular pathologies suggests a possible relationship of Hex with fertilizing capacity.
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spelling pubmed-46371212015-11-13 Seminal plasma hexosaminidase in patients with normal and abnormal spermograms Menendez-Helman, Renata Julia Sanjurjo, Claudia Miranda, Patricia Vivian Iran J Reprod Med Original Article BACKGROUND: Glycosidases profusion in male reproductive fluids suggests a possible relationship with sperm function. Although Hexosaminidase (Hex) is the most active glycosidase in epididymal fluid and seminal plasma, as well as in spermatozoa, Glucosidase is considered a marker for epididymal function and azoospermia. OBJECTIVE: The aim of this study was to determine Hex activity in seminal plasma from patients with normal and abnormal spermograms and analyze its correlation with seminal parameters. MATERIALS AND METHODS: In this cross sectional study, seminal plasma from azoospermic, asthenozoospermic, teratozoospermic, and normozoospermic patients was analyzed for the activity of: total Hex, HexA isoform, and glucosidase. Besides, hexosamine levels were determined, and the amount of Hex was quantified by immunoblot with a specific antibody. Correlation of Hex activity with seminal parameters was also analyzed. RESULTS: Hex activity, like glucosidase, was significantly reduced in azoospermic samples (44, 49, and 60% reduction for total Hex, HexA and glucosidase, respectively). A reduced amount of Hex in azoospermic samples was confirmed by western immunoblot. Hex activity was negatively correlated with round cells in azoospermic samples and positively correlated with motility in asthenozoospermic ones. CONCLUSION: The results suggested that Hex activity was reduced in azoospermic samples and this was due to a lower amount of enzyme. The correlation to seminal parameters related to particular pathologies suggests a possible relationship of Hex with fertilizing capacity. Research and Clinical Center for Infertility 2015-09 /pmc/articles/PMC4637121/ /pubmed/26568758 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Menendez-Helman, Renata Julia
Sanjurjo, Claudia
Miranda, Patricia Vivian
Seminal plasma hexosaminidase in patients with normal and abnormal spermograms
title Seminal plasma hexosaminidase in patients with normal and abnormal spermograms
title_full Seminal plasma hexosaminidase in patients with normal and abnormal spermograms
title_fullStr Seminal plasma hexosaminidase in patients with normal and abnormal spermograms
title_full_unstemmed Seminal plasma hexosaminidase in patients with normal and abnormal spermograms
title_short Seminal plasma hexosaminidase in patients with normal and abnormal spermograms
title_sort seminal plasma hexosaminidase in patients with normal and abnormal spermograms
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4637121/
https://www.ncbi.nlm.nih.gov/pubmed/26568758
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