Cargando…
Development and characterization of 33 novel polymorphic microsatellite markers for the brown tree snake Boiga irregularis
BACKGROUND: Boiga irregularis is a widespread invasive species on Guam and has led to extirpation of most of the island’s native avifauna. There are presently no microsatellite markers for this invasive species, hence we developed highly polymorphic microsatellite markers to allow for robust populat...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4637142/ https://www.ncbi.nlm.nih.gov/pubmed/26547413 http://dx.doi.org/10.1186/s13104-015-1620-z |
_version_ | 1782399767406444544 |
---|---|
author | Unger, Shem D. Abernethy, Erin F. Lance, Stacey L. Beasley, Rochelle R. Kimball, Bruce A. McAuliffe, Thomas W. Jones, Kenneth L. Rhodes, Olin E. |
author_facet | Unger, Shem D. Abernethy, Erin F. Lance, Stacey L. Beasley, Rochelle R. Kimball, Bruce A. McAuliffe, Thomas W. Jones, Kenneth L. Rhodes, Olin E. |
author_sort | Unger, Shem D. |
collection | PubMed |
description | BACKGROUND: Boiga irregularis is a widespread invasive species on Guam and has led to extirpation of most of the island’s native avifauna. There are presently no microsatellite markers for this invasive species, hence we developed highly polymorphic microsatellite markers to allow for robust population genetic studies on Guam. FINDINGS: We isolated and characterized 33 microsatellite loci for the brown tree snake, B. irregularis. The loci were screened across 32 individuals from Guam. The number of alleles per locus ranged from three to ten, with an average of 4.62. The expected (H(e)) and observed heterozygosity (H(o)) ranged from 0.294 to 0.856 and from 0.031 to 0.813, with an average of 0.648 and 0.524, respectively. Significant deviations from Hardy–Weinberg equilibrium were detected at seven loci after Bonferoni correction. Probability of identity values ranged from 0.043 to 0.539. CONCLUSIONS: These genetic markers are useful for understanding a suite of post-invasion population genetic parameters, sources of invasions, and effectiveness of management strategies for this invasive species. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13104-015-1620-z) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4637142 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-46371422015-11-09 Development and characterization of 33 novel polymorphic microsatellite markers for the brown tree snake Boiga irregularis Unger, Shem D. Abernethy, Erin F. Lance, Stacey L. Beasley, Rochelle R. Kimball, Bruce A. McAuliffe, Thomas W. Jones, Kenneth L. Rhodes, Olin E. BMC Res Notes Short Report BACKGROUND: Boiga irregularis is a widespread invasive species on Guam and has led to extirpation of most of the island’s native avifauna. There are presently no microsatellite markers for this invasive species, hence we developed highly polymorphic microsatellite markers to allow for robust population genetic studies on Guam. FINDINGS: We isolated and characterized 33 microsatellite loci for the brown tree snake, B. irregularis. The loci were screened across 32 individuals from Guam. The number of alleles per locus ranged from three to ten, with an average of 4.62. The expected (H(e)) and observed heterozygosity (H(o)) ranged from 0.294 to 0.856 and from 0.031 to 0.813, with an average of 0.648 and 0.524, respectively. Significant deviations from Hardy–Weinberg equilibrium were detected at seven loci after Bonferoni correction. Probability of identity values ranged from 0.043 to 0.539. CONCLUSIONS: These genetic markers are useful for understanding a suite of post-invasion population genetic parameters, sources of invasions, and effectiveness of management strategies for this invasive species. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13104-015-1620-z) contains supplementary material, which is available to authorized users. BioMed Central 2015-11-07 /pmc/articles/PMC4637142/ /pubmed/26547413 http://dx.doi.org/10.1186/s13104-015-1620-z Text en © Unger et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Short Report Unger, Shem D. Abernethy, Erin F. Lance, Stacey L. Beasley, Rochelle R. Kimball, Bruce A. McAuliffe, Thomas W. Jones, Kenneth L. Rhodes, Olin E. Development and characterization of 33 novel polymorphic microsatellite markers for the brown tree snake Boiga irregularis |
title | Development and characterization of 33 novel polymorphic microsatellite markers for the brown tree snake Boiga irregularis |
title_full | Development and characterization of 33 novel polymorphic microsatellite markers for the brown tree snake Boiga irregularis |
title_fullStr | Development and characterization of 33 novel polymorphic microsatellite markers for the brown tree snake Boiga irregularis |
title_full_unstemmed | Development and characterization of 33 novel polymorphic microsatellite markers for the brown tree snake Boiga irregularis |
title_short | Development and characterization of 33 novel polymorphic microsatellite markers for the brown tree snake Boiga irregularis |
title_sort | development and characterization of 33 novel polymorphic microsatellite markers for the brown tree snake boiga irregularis |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4637142/ https://www.ncbi.nlm.nih.gov/pubmed/26547413 http://dx.doi.org/10.1186/s13104-015-1620-z |
work_keys_str_mv | AT ungershemd developmentandcharacterizationof33novelpolymorphicmicrosatellitemarkersforthebrowntreesnakeboigairregularis AT abernethyerinf developmentandcharacterizationof33novelpolymorphicmicrosatellitemarkersforthebrowntreesnakeboigairregularis AT lancestaceyl developmentandcharacterizationof33novelpolymorphicmicrosatellitemarkersforthebrowntreesnakeboigairregularis AT beasleyrocheller developmentandcharacterizationof33novelpolymorphicmicrosatellitemarkersforthebrowntreesnakeboigairregularis AT kimballbrucea developmentandcharacterizationof33novelpolymorphicmicrosatellitemarkersforthebrowntreesnakeboigairregularis AT mcauliffethomasw developmentandcharacterizationof33novelpolymorphicmicrosatellitemarkersforthebrowntreesnakeboigairregularis AT joneskennethl developmentandcharacterizationof33novelpolymorphicmicrosatellitemarkersforthebrowntreesnakeboigairregularis AT rhodesoline developmentandcharacterizationof33novelpolymorphicmicrosatellitemarkersforthebrowntreesnakeboigairregularis |