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Development and characterization of 33 novel polymorphic microsatellite markers for the brown tree snake Boiga irregularis

BACKGROUND: Boiga irregularis is a widespread invasive species on Guam and has led to extirpation of most of the island’s native avifauna. There are presently no microsatellite markers for this invasive species, hence we developed highly polymorphic microsatellite markers to allow for robust populat...

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Autores principales: Unger, Shem D., Abernethy, Erin F., Lance, Stacey L., Beasley, Rochelle R., Kimball, Bruce A., McAuliffe, Thomas W., Jones, Kenneth L., Rhodes, Olin E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4637142/
https://www.ncbi.nlm.nih.gov/pubmed/26547413
http://dx.doi.org/10.1186/s13104-015-1620-z
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author Unger, Shem D.
Abernethy, Erin F.
Lance, Stacey L.
Beasley, Rochelle R.
Kimball, Bruce A.
McAuliffe, Thomas W.
Jones, Kenneth L.
Rhodes, Olin E.
author_facet Unger, Shem D.
Abernethy, Erin F.
Lance, Stacey L.
Beasley, Rochelle R.
Kimball, Bruce A.
McAuliffe, Thomas W.
Jones, Kenneth L.
Rhodes, Olin E.
author_sort Unger, Shem D.
collection PubMed
description BACKGROUND: Boiga irregularis is a widespread invasive species on Guam and has led to extirpation of most of the island’s native avifauna. There are presently no microsatellite markers for this invasive species, hence we developed highly polymorphic microsatellite markers to allow for robust population genetic studies on Guam. FINDINGS: We isolated and characterized 33 microsatellite loci for the brown tree snake, B. irregularis. The loci were screened across 32 individuals from Guam. The number of alleles per locus ranged from three to ten, with an average of 4.62. The expected (H(e)) and observed heterozygosity (H(o)) ranged from 0.294 to 0.856 and from 0.031 to 0.813, with an average of 0.648 and 0.524, respectively. Significant deviations from Hardy–Weinberg equilibrium were detected at seven loci after Bonferoni correction. Probability of identity values ranged from 0.043 to 0.539. CONCLUSIONS: These genetic markers are useful for understanding a suite of post-invasion population genetic parameters, sources of invasions, and effectiveness of management strategies for this invasive species. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13104-015-1620-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-46371422015-11-09 Development and characterization of 33 novel polymorphic microsatellite markers for the brown tree snake Boiga irregularis Unger, Shem D. Abernethy, Erin F. Lance, Stacey L. Beasley, Rochelle R. Kimball, Bruce A. McAuliffe, Thomas W. Jones, Kenneth L. Rhodes, Olin E. BMC Res Notes Short Report BACKGROUND: Boiga irregularis is a widespread invasive species on Guam and has led to extirpation of most of the island’s native avifauna. There are presently no microsatellite markers for this invasive species, hence we developed highly polymorphic microsatellite markers to allow for robust population genetic studies on Guam. FINDINGS: We isolated and characterized 33 microsatellite loci for the brown tree snake, B. irregularis. The loci were screened across 32 individuals from Guam. The number of alleles per locus ranged from three to ten, with an average of 4.62. The expected (H(e)) and observed heterozygosity (H(o)) ranged from 0.294 to 0.856 and from 0.031 to 0.813, with an average of 0.648 and 0.524, respectively. Significant deviations from Hardy–Weinberg equilibrium were detected at seven loci after Bonferoni correction. Probability of identity values ranged from 0.043 to 0.539. CONCLUSIONS: These genetic markers are useful for understanding a suite of post-invasion population genetic parameters, sources of invasions, and effectiveness of management strategies for this invasive species. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13104-015-1620-z) contains supplementary material, which is available to authorized users. BioMed Central 2015-11-07 /pmc/articles/PMC4637142/ /pubmed/26547413 http://dx.doi.org/10.1186/s13104-015-1620-z Text en © Unger et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
Unger, Shem D.
Abernethy, Erin F.
Lance, Stacey L.
Beasley, Rochelle R.
Kimball, Bruce A.
McAuliffe, Thomas W.
Jones, Kenneth L.
Rhodes, Olin E.
Development and characterization of 33 novel polymorphic microsatellite markers for the brown tree snake Boiga irregularis
title Development and characterization of 33 novel polymorphic microsatellite markers for the brown tree snake Boiga irregularis
title_full Development and characterization of 33 novel polymorphic microsatellite markers for the brown tree snake Boiga irregularis
title_fullStr Development and characterization of 33 novel polymorphic microsatellite markers for the brown tree snake Boiga irregularis
title_full_unstemmed Development and characterization of 33 novel polymorphic microsatellite markers for the brown tree snake Boiga irregularis
title_short Development and characterization of 33 novel polymorphic microsatellite markers for the brown tree snake Boiga irregularis
title_sort development and characterization of 33 novel polymorphic microsatellite markers for the brown tree snake boiga irregularis
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4637142/
https://www.ncbi.nlm.nih.gov/pubmed/26547413
http://dx.doi.org/10.1186/s13104-015-1620-z
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