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A Novel Mouse Model of Peritoneal Dialysis: Combination of Uraemia and Long-Term Exposure to PD Fluid

Different animal models for peritoneal dialysis (PD) have been used in the past decades to develop PD fluids compatible with patient life and to identify markers of peritoneal fibrosis and inflammation. Only few of those studies have taken into account the importance of uraemia-induced alterations a...

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Autores principales: Ferrantelli, E., Liappas, G., Keuning, E. D., Vila Cuenca, M., González-Mateo, G., Verkaik, M., López-Cabrera, M., Beelen, R. H. J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4637431/
https://www.ncbi.nlm.nih.gov/pubmed/26587530
http://dx.doi.org/10.1155/2015/106902
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author Ferrantelli, E.
Liappas, G.
Keuning, E. D.
Vila Cuenca, M.
González-Mateo, G.
Verkaik, M.
López-Cabrera, M.
Beelen, R. H. J.
author_facet Ferrantelli, E.
Liappas, G.
Keuning, E. D.
Vila Cuenca, M.
González-Mateo, G.
Verkaik, M.
López-Cabrera, M.
Beelen, R. H. J.
author_sort Ferrantelli, E.
collection PubMed
description Different animal models for peritoneal dialysis (PD) have been used in the past decades to develop PD fluids compatible with patient life and to identify markers of peritoneal fibrosis and inflammation. Only few of those studies have taken into account the importance of uraemia-induced alterations at both systemic and peritoneal levels. Moreover, some animal studies which have reported about PD in a uremic setting did not always entirely succeed in terms of uraemia establishment and animal survival. In the present study we induced uraemia in the recently established mouse PD exposure model in order to obtain a more clinically relevant mouse model for kidney patients. This new designed model reflected both the slight thickening of peritoneal membrane induced by uraemia and the significant extracellular matrix deposition due to daily PD fluid instillation. In addition the model offers the opportunity to perform long-term exposure to PD fluids, as it is observed in the clinical setting, and gives the advantage to knock out candidate markers for driving peritoneal inflammatory mechanisms.
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spelling pubmed-46374312015-11-19 A Novel Mouse Model of Peritoneal Dialysis: Combination of Uraemia and Long-Term Exposure to PD Fluid Ferrantelli, E. Liappas, G. Keuning, E. D. Vila Cuenca, M. González-Mateo, G. Verkaik, M. López-Cabrera, M. Beelen, R. H. J. Biomed Res Int Research Article Different animal models for peritoneal dialysis (PD) have been used in the past decades to develop PD fluids compatible with patient life and to identify markers of peritoneal fibrosis and inflammation. Only few of those studies have taken into account the importance of uraemia-induced alterations at both systemic and peritoneal levels. Moreover, some animal studies which have reported about PD in a uremic setting did not always entirely succeed in terms of uraemia establishment and animal survival. In the present study we induced uraemia in the recently established mouse PD exposure model in order to obtain a more clinically relevant mouse model for kidney patients. This new designed model reflected both the slight thickening of peritoneal membrane induced by uraemia and the significant extracellular matrix deposition due to daily PD fluid instillation. In addition the model offers the opportunity to perform long-term exposure to PD fluids, as it is observed in the clinical setting, and gives the advantage to knock out candidate markers for driving peritoneal inflammatory mechanisms. Hindawi Publishing Corporation 2015 2015-10-26 /pmc/articles/PMC4637431/ /pubmed/26587530 http://dx.doi.org/10.1155/2015/106902 Text en Copyright © 2015 E. Ferrantelli et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ferrantelli, E.
Liappas, G.
Keuning, E. D.
Vila Cuenca, M.
González-Mateo, G.
Verkaik, M.
López-Cabrera, M.
Beelen, R. H. J.
A Novel Mouse Model of Peritoneal Dialysis: Combination of Uraemia and Long-Term Exposure to PD Fluid
title A Novel Mouse Model of Peritoneal Dialysis: Combination of Uraemia and Long-Term Exposure to PD Fluid
title_full A Novel Mouse Model of Peritoneal Dialysis: Combination of Uraemia and Long-Term Exposure to PD Fluid
title_fullStr A Novel Mouse Model of Peritoneal Dialysis: Combination of Uraemia and Long-Term Exposure to PD Fluid
title_full_unstemmed A Novel Mouse Model of Peritoneal Dialysis: Combination of Uraemia and Long-Term Exposure to PD Fluid
title_short A Novel Mouse Model of Peritoneal Dialysis: Combination of Uraemia and Long-Term Exposure to PD Fluid
title_sort novel mouse model of peritoneal dialysis: combination of uraemia and long-term exposure to pd fluid
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4637431/
https://www.ncbi.nlm.nih.gov/pubmed/26587530
http://dx.doi.org/10.1155/2015/106902
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