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Superinfection exclusion is absent during acute Junin virus infection of Vero and A549 cells

Many viruses have evolved strategies of so-called “superinfection exclusion” to prevent re-infection of a cell that the same virus has already infected. Although Old World arenavirus infection results in down-regulation of its viral receptor and thus superinfection exclusion, whether New World arena...

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Autores principales: Gaudin, Raphaël, Kirchhausen, Tomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4637830/
https://www.ncbi.nlm.nih.gov/pubmed/26549784
http://dx.doi.org/10.1038/srep15990
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author Gaudin, Raphaël
Kirchhausen, Tomas
author_facet Gaudin, Raphaël
Kirchhausen, Tomas
author_sort Gaudin, Raphaël
collection PubMed
description Many viruses have evolved strategies of so-called “superinfection exclusion” to prevent re-infection of a cell that the same virus has already infected. Although Old World arenavirus infection results in down-regulation of its viral receptor and thus superinfection exclusion, whether New World arenaviruses have evolved such a mechanism remains unclear. Here we show that acute infection by the New World Junin virus (JUNV) failed to down-regulate the transferrin receptor and did not induce superinfection exclusion. We observed that Vero cells infected by a first round of JUNV (Candid1 strain) preserve an ability to internalize new incoming JUNV particles that is comparable to that of non-infected cells. Moreover, we developed a dual infection assay with the wild-type Candid1 JUNV and a recombinant JUNV-GFP virus to discriminate between first and second infections at the transcriptional and translational levels. We found that Vero and A549 cells already infected by JUNV were fully competent to transcribe viral RNA from a second round of infection. Furthermore, flow cytometry analysis of viral protein expression indicated that viral translation was normal, regardless of whether cells were previously infected or not. We conclude that in acutely infected cells, Junin virus lacks a superinfection exclusion mechanism.
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spelling pubmed-46378302015-11-30 Superinfection exclusion is absent during acute Junin virus infection of Vero and A549 cells Gaudin, Raphaël Kirchhausen, Tomas Sci Rep Article Many viruses have evolved strategies of so-called “superinfection exclusion” to prevent re-infection of a cell that the same virus has already infected. Although Old World arenavirus infection results in down-regulation of its viral receptor and thus superinfection exclusion, whether New World arenaviruses have evolved such a mechanism remains unclear. Here we show that acute infection by the New World Junin virus (JUNV) failed to down-regulate the transferrin receptor and did not induce superinfection exclusion. We observed that Vero cells infected by a first round of JUNV (Candid1 strain) preserve an ability to internalize new incoming JUNV particles that is comparable to that of non-infected cells. Moreover, we developed a dual infection assay with the wild-type Candid1 JUNV and a recombinant JUNV-GFP virus to discriminate between first and second infections at the transcriptional and translational levels. We found that Vero and A549 cells already infected by JUNV were fully competent to transcribe viral RNA from a second round of infection. Furthermore, flow cytometry analysis of viral protein expression indicated that viral translation was normal, regardless of whether cells were previously infected or not. We conclude that in acutely infected cells, Junin virus lacks a superinfection exclusion mechanism. Nature Publishing Group 2015-11-09 /pmc/articles/PMC4637830/ /pubmed/26549784 http://dx.doi.org/10.1038/srep15990 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Gaudin, Raphaël
Kirchhausen, Tomas
Superinfection exclusion is absent during acute Junin virus infection of Vero and A549 cells
title Superinfection exclusion is absent during acute Junin virus infection of Vero and A549 cells
title_full Superinfection exclusion is absent during acute Junin virus infection of Vero and A549 cells
title_fullStr Superinfection exclusion is absent during acute Junin virus infection of Vero and A549 cells
title_full_unstemmed Superinfection exclusion is absent during acute Junin virus infection of Vero and A549 cells
title_short Superinfection exclusion is absent during acute Junin virus infection of Vero and A549 cells
title_sort superinfection exclusion is absent during acute junin virus infection of vero and a549 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4637830/
https://www.ncbi.nlm.nih.gov/pubmed/26549784
http://dx.doi.org/10.1038/srep15990
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