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Mitochondria-related miR-141-3p contributes to mitochondrial dysfunction in HFD-induced obesity by inhibiting PTEN

Mitochondria-related microRNAs (miRNAs) have recently emerged as key regulators of cell metabolism and can modulate mitochondrial fusion and division. In order to investigate the roles of mitochondria-related miRNAs played in obesity, we conducted comprehensive molecular analysis in vitro and in viv...

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Autores principales: Ji, Juan, Qin, Yufeng, Ren, Jing, Lu, Chuncheng, Wang, Rong, Dai, Xiuliang, Zhou, Ran, Huang, Zhenyao, Xu, Miaofei, Chen, Minjian, Wu, Wei, Song, Ling, Shen, Hongbing, Hu, Zhibin, Miao, Dengshun, Xia, Yankai, Wang, Xinru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4637860/
https://www.ncbi.nlm.nih.gov/pubmed/26548909
http://dx.doi.org/10.1038/srep16262
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author Ji, Juan
Qin, Yufeng
Ren, Jing
Lu, Chuncheng
Wang, Rong
Dai, Xiuliang
Zhou, Ran
Huang, Zhenyao
Xu, Miaofei
Chen, Minjian
Wu, Wei
Song, Ling
Shen, Hongbing
Hu, Zhibin
Miao, Dengshun
Xia, Yankai
Wang, Xinru
author_facet Ji, Juan
Qin, Yufeng
Ren, Jing
Lu, Chuncheng
Wang, Rong
Dai, Xiuliang
Zhou, Ran
Huang, Zhenyao
Xu, Miaofei
Chen, Minjian
Wu, Wei
Song, Ling
Shen, Hongbing
Hu, Zhibin
Miao, Dengshun
Xia, Yankai
Wang, Xinru
author_sort Ji, Juan
collection PubMed
description Mitochondria-related microRNAs (miRNAs) have recently emerged as key regulators of cell metabolism and can modulate mitochondrial fusion and division. In order to investigate the roles of mitochondria-related miRNAs played in obesity, we conducted comprehensive molecular analysis in vitro and in vivo. Based on high-fat-diet (HFD) induced obese mice, we found that hepatic mitochondrial function was markedly altered. Subsequently, we evaluated the expression levels of selected mitochondria-related miRNAs and found that miR-141-3p was up-regulated strikingly in HFD mice. To further verify the role of miR-141-3p in obesity, we carried out gain-and-loss-of-function study in human HepG2 cells. We found that miR-141-3p could modulate ATP production and induce oxidative stress. Through luciferase report gene assay, we identified that phosphatase and tensin homolog (PTEN) was a target of miR-141-3p. Inhibiting PTEN could alter the mitochondrial function, too. Our study suggested that mitochondria-related miR-141-3p induced mitochondrial dysfunction by inhibiting PTEN.
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spelling pubmed-46378602015-11-30 Mitochondria-related miR-141-3p contributes to mitochondrial dysfunction in HFD-induced obesity by inhibiting PTEN Ji, Juan Qin, Yufeng Ren, Jing Lu, Chuncheng Wang, Rong Dai, Xiuliang Zhou, Ran Huang, Zhenyao Xu, Miaofei Chen, Minjian Wu, Wei Song, Ling Shen, Hongbing Hu, Zhibin Miao, Dengshun Xia, Yankai Wang, Xinru Sci Rep Article Mitochondria-related microRNAs (miRNAs) have recently emerged as key regulators of cell metabolism and can modulate mitochondrial fusion and division. In order to investigate the roles of mitochondria-related miRNAs played in obesity, we conducted comprehensive molecular analysis in vitro and in vivo. Based on high-fat-diet (HFD) induced obese mice, we found that hepatic mitochondrial function was markedly altered. Subsequently, we evaluated the expression levels of selected mitochondria-related miRNAs and found that miR-141-3p was up-regulated strikingly in HFD mice. To further verify the role of miR-141-3p in obesity, we carried out gain-and-loss-of-function study in human HepG2 cells. We found that miR-141-3p could modulate ATP production and induce oxidative stress. Through luciferase report gene assay, we identified that phosphatase and tensin homolog (PTEN) was a target of miR-141-3p. Inhibiting PTEN could alter the mitochondrial function, too. Our study suggested that mitochondria-related miR-141-3p induced mitochondrial dysfunction by inhibiting PTEN. Nature Publishing Group 2015-11-09 /pmc/articles/PMC4637860/ /pubmed/26548909 http://dx.doi.org/10.1038/srep16262 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Ji, Juan
Qin, Yufeng
Ren, Jing
Lu, Chuncheng
Wang, Rong
Dai, Xiuliang
Zhou, Ran
Huang, Zhenyao
Xu, Miaofei
Chen, Minjian
Wu, Wei
Song, Ling
Shen, Hongbing
Hu, Zhibin
Miao, Dengshun
Xia, Yankai
Wang, Xinru
Mitochondria-related miR-141-3p contributes to mitochondrial dysfunction in HFD-induced obesity by inhibiting PTEN
title Mitochondria-related miR-141-3p contributes to mitochondrial dysfunction in HFD-induced obesity by inhibiting PTEN
title_full Mitochondria-related miR-141-3p contributes to mitochondrial dysfunction in HFD-induced obesity by inhibiting PTEN
title_fullStr Mitochondria-related miR-141-3p contributes to mitochondrial dysfunction in HFD-induced obesity by inhibiting PTEN
title_full_unstemmed Mitochondria-related miR-141-3p contributes to mitochondrial dysfunction in HFD-induced obesity by inhibiting PTEN
title_short Mitochondria-related miR-141-3p contributes to mitochondrial dysfunction in HFD-induced obesity by inhibiting PTEN
title_sort mitochondria-related mir-141-3p contributes to mitochondrial dysfunction in hfd-induced obesity by inhibiting pten
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4637860/
https://www.ncbi.nlm.nih.gov/pubmed/26548909
http://dx.doi.org/10.1038/srep16262
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