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Sendai virus-mediated expression of reprogramming factors promotes plasticity of human neuroblastoma cells

Neuroblastoma (NB) is the most common extracranial solid tumor that originates from multipotent neural crest cells. NB cell populations that express embryonic stem cell-associated genes have been identified and shown to retain a multipotent phenotype. However, whether somatic reprogramming of NB cel...

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Autores principales: Islam, S M Rafiqul, Suenaga, Yusuke, Takatori, Atsushi, Ueda, Yasuji, Kaneko, Yoshiki, Kawana, Hidetada, Itami, Makiko, Ohira, Miki, Yokoi, Sana, Nakagawara, Akira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4638011/
https://www.ncbi.nlm.nih.gov/pubmed/26190440
http://dx.doi.org/10.1111/cas.12746
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author Islam, S M Rafiqul
Suenaga, Yusuke
Takatori, Atsushi
Ueda, Yasuji
Kaneko, Yoshiki
Kawana, Hidetada
Itami, Makiko
Ohira, Miki
Yokoi, Sana
Nakagawara, Akira
author_facet Islam, S M Rafiqul
Suenaga, Yusuke
Takatori, Atsushi
Ueda, Yasuji
Kaneko, Yoshiki
Kawana, Hidetada
Itami, Makiko
Ohira, Miki
Yokoi, Sana
Nakagawara, Akira
author_sort Islam, S M Rafiqul
collection PubMed
description Neuroblastoma (NB) is the most common extracranial solid tumor that originates from multipotent neural crest cells. NB cell populations that express embryonic stem cell-associated genes have been identified and shown to retain a multipotent phenotype. However, whether somatic reprogramming of NB cells can produce similar stem-cell like populations is unknown. Here, we sought to reprogram NB cell lines using an integration-free Sendai virus vector system. Of four NB cell lines examined, only SH-IN cells formed induced pluripotent stem cell-like colonies (SH-IN 4F colonies) at approximately 6 weeks following transduction. These SH-IN 4F colonies were alkaline phosphatase-positive. Array comparative genomic hybridization analysis indicated identical genomic aberrations in the SH-IN 4F cells as in the parental cells. SH-IN 4F cells had the ability to differentiate into the three embryonic germ layers in vitro, but rather formed NBs in vivo. Furthermore, SH-IN 4F cells exhibited resistance to cisplatin treatment and differentiated into endothelial-like cells expressing CD31 in the presence of vascular endothelial growth factor. These results suggest that SH-IN 4F cells are partially reprogrammed NB cells, and could be a suitable model for investigating the plasticity of aggressive tumors.
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spelling pubmed-46380112015-11-12 Sendai virus-mediated expression of reprogramming factors promotes plasticity of human neuroblastoma cells Islam, S M Rafiqul Suenaga, Yusuke Takatori, Atsushi Ueda, Yasuji Kaneko, Yoshiki Kawana, Hidetada Itami, Makiko Ohira, Miki Yokoi, Sana Nakagawara, Akira Cancer Sci Original Articles Neuroblastoma (NB) is the most common extracranial solid tumor that originates from multipotent neural crest cells. NB cell populations that express embryonic stem cell-associated genes have been identified and shown to retain a multipotent phenotype. However, whether somatic reprogramming of NB cells can produce similar stem-cell like populations is unknown. Here, we sought to reprogram NB cell lines using an integration-free Sendai virus vector system. Of four NB cell lines examined, only SH-IN cells formed induced pluripotent stem cell-like colonies (SH-IN 4F colonies) at approximately 6 weeks following transduction. These SH-IN 4F colonies were alkaline phosphatase-positive. Array comparative genomic hybridization analysis indicated identical genomic aberrations in the SH-IN 4F cells as in the parental cells. SH-IN 4F cells had the ability to differentiate into the three embryonic germ layers in vitro, but rather formed NBs in vivo. Furthermore, SH-IN 4F cells exhibited resistance to cisplatin treatment and differentiated into endothelial-like cells expressing CD31 in the presence of vascular endothelial growth factor. These results suggest that SH-IN 4F cells are partially reprogrammed NB cells, and could be a suitable model for investigating the plasticity of aggressive tumors. John Wiley & Sons, Ltd 2015-10 2015-08-18 /pmc/articles/PMC4638011/ /pubmed/26190440 http://dx.doi.org/10.1111/cas.12746 Text en © 2015 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association. http://creativecommons.org/licenses/by-nc/4.0/ This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Islam, S M Rafiqul
Suenaga, Yusuke
Takatori, Atsushi
Ueda, Yasuji
Kaneko, Yoshiki
Kawana, Hidetada
Itami, Makiko
Ohira, Miki
Yokoi, Sana
Nakagawara, Akira
Sendai virus-mediated expression of reprogramming factors promotes plasticity of human neuroblastoma cells
title Sendai virus-mediated expression of reprogramming factors promotes plasticity of human neuroblastoma cells
title_full Sendai virus-mediated expression of reprogramming factors promotes plasticity of human neuroblastoma cells
title_fullStr Sendai virus-mediated expression of reprogramming factors promotes plasticity of human neuroblastoma cells
title_full_unstemmed Sendai virus-mediated expression of reprogramming factors promotes plasticity of human neuroblastoma cells
title_short Sendai virus-mediated expression of reprogramming factors promotes plasticity of human neuroblastoma cells
title_sort sendai virus-mediated expression of reprogramming factors promotes plasticity of human neuroblastoma cells
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4638011/
https://www.ncbi.nlm.nih.gov/pubmed/26190440
http://dx.doi.org/10.1111/cas.12746
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