Cargando…
Aqueous extract of Rabdosia rubescens leaves: forming nanoparticles, targeting P-selectin, and inhibiting thrombosis
The hot water extract of Rabdosia rubescens was traditionally used as an antithrombotic medicine. To explore its antithrombotic utility and mechanism, we carried out a series of in vitro and in vivo assays in this study. In vitro platelet aggregation assay showed that the half maximal inhibitory con...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4639563/ https://www.ncbi.nlm.nih.gov/pubmed/26604756 http://dx.doi.org/10.2147/IJN.S91316 |
_version_ | 1782399938129297408 |
---|---|
author | Wang, Yuji Tang, Jingcheng Zhu, Haimei Jiang, Xueyun Liu, Jiawang Xu, Wenyun Ma, Haiping Feng, Qiqi Wu, Jianhui Zhao, Ming Peng, Shiqi |
author_facet | Wang, Yuji Tang, Jingcheng Zhu, Haimei Jiang, Xueyun Liu, Jiawang Xu, Wenyun Ma, Haiping Feng, Qiqi Wu, Jianhui Zhao, Ming Peng, Shiqi |
author_sort | Wang, Yuji |
collection | PubMed |
description | The hot water extract of Rabdosia rubescens was traditionally used as an antithrombotic medicine. To explore its antithrombotic utility and mechanism, we carried out a series of in vitro and in vivo assays in this study. In vitro platelet aggregation assay showed that the half maximal inhibitory concentration values of aqueous extract of R. rubescens leaves (AERL) inhibiting platelet aggregation induced by thrombin, arachidonic acid, adenosine diphosphate, and platelet-activating factor ranged from 0.12 mg/mL to 1.43 mg/mL. The minimal effective oral dose of AERL inhibiting the rats from forming thrombus was 25 mg/kg. Both in vitro and in vivo actions were correlated with AERL concentration-dependently inhibiting sP-selectin release. In water, AERL formed nanoparticles, and their size depended on the concentration. Docking the five nucleotides, 21 phenolic acids, and four diterpenoids identified by high-performance liquid chromatography–photodiode array detector/(−)electrospray ionization-tandem mass spectrometry analysis into the active site of P-selectin, rosmarinic acid was predicted to be the antithrombotic ingredient of AERL. In flow cytometry analysis, 1 μM of rosmarinic acid effectively inhibited sP-selectin release in arachidonic acid-activated platelets. In a rat model, 5 mg/kg of oral rosmarinic acid effectively inhibited thrombosis. |
format | Online Article Text |
id | pubmed-4639563 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-46395632015-11-24 Aqueous extract of Rabdosia rubescens leaves: forming nanoparticles, targeting P-selectin, and inhibiting thrombosis Wang, Yuji Tang, Jingcheng Zhu, Haimei Jiang, Xueyun Liu, Jiawang Xu, Wenyun Ma, Haiping Feng, Qiqi Wu, Jianhui Zhao, Ming Peng, Shiqi Int J Nanomedicine Original Research The hot water extract of Rabdosia rubescens was traditionally used as an antithrombotic medicine. To explore its antithrombotic utility and mechanism, we carried out a series of in vitro and in vivo assays in this study. In vitro platelet aggregation assay showed that the half maximal inhibitory concentration values of aqueous extract of R. rubescens leaves (AERL) inhibiting platelet aggregation induced by thrombin, arachidonic acid, adenosine diphosphate, and platelet-activating factor ranged from 0.12 mg/mL to 1.43 mg/mL. The minimal effective oral dose of AERL inhibiting the rats from forming thrombus was 25 mg/kg. Both in vitro and in vivo actions were correlated with AERL concentration-dependently inhibiting sP-selectin release. In water, AERL formed nanoparticles, and their size depended on the concentration. Docking the five nucleotides, 21 phenolic acids, and four diterpenoids identified by high-performance liquid chromatography–photodiode array detector/(−)electrospray ionization-tandem mass spectrometry analysis into the active site of P-selectin, rosmarinic acid was predicted to be the antithrombotic ingredient of AERL. In flow cytometry analysis, 1 μM of rosmarinic acid effectively inhibited sP-selectin release in arachidonic acid-activated platelets. In a rat model, 5 mg/kg of oral rosmarinic acid effectively inhibited thrombosis. Dove Medical Press 2015-11-04 /pmc/articles/PMC4639563/ /pubmed/26604756 http://dx.doi.org/10.2147/IJN.S91316 Text en © 2015 Wang et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Wang, Yuji Tang, Jingcheng Zhu, Haimei Jiang, Xueyun Liu, Jiawang Xu, Wenyun Ma, Haiping Feng, Qiqi Wu, Jianhui Zhao, Ming Peng, Shiqi Aqueous extract of Rabdosia rubescens leaves: forming nanoparticles, targeting P-selectin, and inhibiting thrombosis |
title | Aqueous extract of Rabdosia rubescens leaves: forming nanoparticles, targeting P-selectin, and inhibiting thrombosis |
title_full | Aqueous extract of Rabdosia rubescens leaves: forming nanoparticles, targeting P-selectin, and inhibiting thrombosis |
title_fullStr | Aqueous extract of Rabdosia rubescens leaves: forming nanoparticles, targeting P-selectin, and inhibiting thrombosis |
title_full_unstemmed | Aqueous extract of Rabdosia rubescens leaves: forming nanoparticles, targeting P-selectin, and inhibiting thrombosis |
title_short | Aqueous extract of Rabdosia rubescens leaves: forming nanoparticles, targeting P-selectin, and inhibiting thrombosis |
title_sort | aqueous extract of rabdosia rubescens leaves: forming nanoparticles, targeting p-selectin, and inhibiting thrombosis |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4639563/ https://www.ncbi.nlm.nih.gov/pubmed/26604756 http://dx.doi.org/10.2147/IJN.S91316 |
work_keys_str_mv | AT wangyuji aqueousextractofrabdosiarubescensleavesformingnanoparticlestargetingpselectinandinhibitingthrombosis AT tangjingcheng aqueousextractofrabdosiarubescensleavesformingnanoparticlestargetingpselectinandinhibitingthrombosis AT zhuhaimei aqueousextractofrabdosiarubescensleavesformingnanoparticlestargetingpselectinandinhibitingthrombosis AT jiangxueyun aqueousextractofrabdosiarubescensleavesformingnanoparticlestargetingpselectinandinhibitingthrombosis AT liujiawang aqueousextractofrabdosiarubescensleavesformingnanoparticlestargetingpselectinandinhibitingthrombosis AT xuwenyun aqueousextractofrabdosiarubescensleavesformingnanoparticlestargetingpselectinandinhibitingthrombosis AT mahaiping aqueousextractofrabdosiarubescensleavesformingnanoparticlestargetingpselectinandinhibitingthrombosis AT fengqiqi aqueousextractofrabdosiarubescensleavesformingnanoparticlestargetingpselectinandinhibitingthrombosis AT wujianhui aqueousextractofrabdosiarubescensleavesformingnanoparticlestargetingpselectinandinhibitingthrombosis AT zhaoming aqueousextractofrabdosiarubescensleavesformingnanoparticlestargetingpselectinandinhibitingthrombosis AT pengshiqi aqueousextractofrabdosiarubescensleavesformingnanoparticlestargetingpselectinandinhibitingthrombosis |