Cargando…
Topological control of nitric oxide secretion by tantalum oxide nanodot arrays
BACKGROUND: Nitric oxide (NO) plays a very important role in the cardiovascular system as a major secondary messenger in signaling pathway. Its concentration regulates most of the important physiological indexes including the systemic blood pressure, blood flow, regional vascular tone and other card...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640104/ https://www.ncbi.nlm.nih.gov/pubmed/26553043 http://dx.doi.org/10.1186/s12951-015-0144-y |
_version_ | 1782400034385428480 |
---|---|
author | Dhawan, Udesh Lee, Chia Hui Huang, Chun-Chung Chu, Ying Hao Huang, Guewha S. Lin, Yan-Ren Chen, Wen-Liang |
author_facet | Dhawan, Udesh Lee, Chia Hui Huang, Chun-Chung Chu, Ying Hao Huang, Guewha S. Lin, Yan-Ren Chen, Wen-Liang |
author_sort | Dhawan, Udesh |
collection | PubMed |
description | BACKGROUND: Nitric oxide (NO) plays a very important role in the cardiovascular system as a major secondary messenger in signaling pathway. Its concentration regulates most of the important physiological indexes including the systemic blood pressure, blood flow, regional vascular tone and other cardiac functions. The effect of nanotopography on the NO secretion in cardiomyocytes has not been elucidated before. In this study, we report how the nanotopography can modulate the secretion profile of NO and attempt to elucidate the genetic pathways responsible for the same by using Tantalum Oxide nanodot arrays ranging from 10 to 200 nm. A series of nanodot arrays were fabricated with dot diameter ranging from 10 to 200 nm. Temporal NO release of cardiomyocytes was quantified when grown on different surfaces. Quantitative RT-PCR and Western blot were performed to verify the genetic pathways of NO release. RESULTS: After hours 24 of cell seeding, NO release was slowly enhanced by the increase of dot diameter from 10 nm up to 50 nm, mildly enhanced to a medium level at 100 nm, and increase rapidly to a high level at 200 nm. The temporal enhancement of NO release dropped dramatically on day 3. On day 5, a topology-dependent profile was established that maximized at 50 nm and dropped to control level at 200 nm. The NO releasing profile was closely associated with the expression patterns of genes associated with Endothelial nitric oxide synthase (eNOS) pathway [GPCR, PI3K, Akt, Bad, Bcl-2, NFκB(p65), eNOS], but less associated with Inducible nitric oxide synthase (iNOS) pathway (TNF-α, ILK, Akt, IκBα, NFκB, iNOS). Western blotting of Akt, eNOS, iNOS, and NFκB further validated that eNOS pathway was modulated by nanotopology. CONCLUSIONS: Based on the findings of the present study, 50, 100 nm can serve as the suitable nanotopography patterns for cardiac implant surface design. These two nanodot arrays promote NO secretion and can also promote the vascular smooth muscle relaxation. The results of this study can improve the heart stent design in the medical treatments. |
format | Online Article Text |
id | pubmed-4640104 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-46401042015-11-11 Topological control of nitric oxide secretion by tantalum oxide nanodot arrays Dhawan, Udesh Lee, Chia Hui Huang, Chun-Chung Chu, Ying Hao Huang, Guewha S. Lin, Yan-Ren Chen, Wen-Liang J Nanobiotechnology Research BACKGROUND: Nitric oxide (NO) plays a very important role in the cardiovascular system as a major secondary messenger in signaling pathway. Its concentration regulates most of the important physiological indexes including the systemic blood pressure, blood flow, regional vascular tone and other cardiac functions. The effect of nanotopography on the NO secretion in cardiomyocytes has not been elucidated before. In this study, we report how the nanotopography can modulate the secretion profile of NO and attempt to elucidate the genetic pathways responsible for the same by using Tantalum Oxide nanodot arrays ranging from 10 to 200 nm. A series of nanodot arrays were fabricated with dot diameter ranging from 10 to 200 nm. Temporal NO release of cardiomyocytes was quantified when grown on different surfaces. Quantitative RT-PCR and Western blot were performed to verify the genetic pathways of NO release. RESULTS: After hours 24 of cell seeding, NO release was slowly enhanced by the increase of dot diameter from 10 nm up to 50 nm, mildly enhanced to a medium level at 100 nm, and increase rapidly to a high level at 200 nm. The temporal enhancement of NO release dropped dramatically on day 3. On day 5, a topology-dependent profile was established that maximized at 50 nm and dropped to control level at 200 nm. The NO releasing profile was closely associated with the expression patterns of genes associated with Endothelial nitric oxide synthase (eNOS) pathway [GPCR, PI3K, Akt, Bad, Bcl-2, NFκB(p65), eNOS], but less associated with Inducible nitric oxide synthase (iNOS) pathway (TNF-α, ILK, Akt, IκBα, NFκB, iNOS). Western blotting of Akt, eNOS, iNOS, and NFκB further validated that eNOS pathway was modulated by nanotopology. CONCLUSIONS: Based on the findings of the present study, 50, 100 nm can serve as the suitable nanotopography patterns for cardiac implant surface design. These two nanodot arrays promote NO secretion and can also promote the vascular smooth muscle relaxation. The results of this study can improve the heart stent design in the medical treatments. BioMed Central 2015-11-09 /pmc/articles/PMC4640104/ /pubmed/26553043 http://dx.doi.org/10.1186/s12951-015-0144-y Text en © Dhawan et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Dhawan, Udesh Lee, Chia Hui Huang, Chun-Chung Chu, Ying Hao Huang, Guewha S. Lin, Yan-Ren Chen, Wen-Liang Topological control of nitric oxide secretion by tantalum oxide nanodot arrays |
title | Topological control of nitric oxide secretion by tantalum oxide nanodot arrays |
title_full | Topological control of nitric oxide secretion by tantalum oxide nanodot arrays |
title_fullStr | Topological control of nitric oxide secretion by tantalum oxide nanodot arrays |
title_full_unstemmed | Topological control of nitric oxide secretion by tantalum oxide nanodot arrays |
title_short | Topological control of nitric oxide secretion by tantalum oxide nanodot arrays |
title_sort | topological control of nitric oxide secretion by tantalum oxide nanodot arrays |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640104/ https://www.ncbi.nlm.nih.gov/pubmed/26553043 http://dx.doi.org/10.1186/s12951-015-0144-y |
work_keys_str_mv | AT dhawanudesh topologicalcontrolofnitricoxidesecretionbytantalumoxidenanodotarrays AT leechiahui topologicalcontrolofnitricoxidesecretionbytantalumoxidenanodotarrays AT huangchunchung topologicalcontrolofnitricoxidesecretionbytantalumoxidenanodotarrays AT chuyinghao topologicalcontrolofnitricoxidesecretionbytantalumoxidenanodotarrays AT huangguewhas topologicalcontrolofnitricoxidesecretionbytantalumoxidenanodotarrays AT linyanren topologicalcontrolofnitricoxidesecretionbytantalumoxidenanodotarrays AT chenwenliang topologicalcontrolofnitricoxidesecretionbytantalumoxidenanodotarrays |