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Mussel processing wastewater: a low-cost substrate for the production of astaxanthin by Xanthophyllomyces dendrorhous

BACKGROUND: The use of astaxanthin in different industries such as the chemical, pharmaceutical, food, animal feed and cosmetic has been receiving increasing attention in recent years. Natural supplies of the pigment include crustacean by-products, algal, and microbial cultivation, being the yeast X...

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Autores principales: Amado, Isabel Rodríguez, Vázquez, José Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640366/
https://www.ncbi.nlm.nih.gov/pubmed/26553283
http://dx.doi.org/10.1186/s12934-015-0375-5
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author Amado, Isabel Rodríguez
Vázquez, José Antonio
author_facet Amado, Isabel Rodríguez
Vázquez, José Antonio
author_sort Amado, Isabel Rodríguez
collection PubMed
description BACKGROUND: The use of astaxanthin in different industries such as the chemical, pharmaceutical, food, animal feed and cosmetic has been receiving increasing attention in recent years. Natural supplies of the pigment include crustacean by-products, algal, and microbial cultivation, being the yeast Xanthophyllomyces dendrorhous together with the alga Haematococcus pluvialis the most promising microorganisms for this bioproduction. Different vegetable by-products of the food industry have been explored so far as low-cost substrates for the production of astaxanthin by X. dendrorhous. This study focuses for the first time on the use of a low-cost formulated medium from a marine by-product, mussel-processing wastewater, for the production of astaxanthin by the yeast X. dendrorhous. RESULTS: The yeast was able to grow in non-saccharified mussel broth, revealing the ability of the microorganism to hydrolyze glycogen. However, partial glycogen saccharification with α-amylase was needed for astaxanthin biosynthesis, obtaining maximal productions of 22.5–26.0 mg/L towards the end of the culture and coinciding with yeast highest amylolytic activity. Cultivations in totally-saccharified media revealed an increase in maximal cell concentrations and a decrease in maximal growth rates and astaxanthin production with increasing glucose initial concentration. CONCLUSIONS: Astaxanthin production was higher in partially-saccharified mussel-processing waste than in synthetic medium (yeast peptone dextrose) containing glucose as carbon source (13 mg/L), suggesting this by-product is a promising nutritive medium for astaxanthin production. The use of this effluent also contributes towards the recycling and depuration of this highly pollutant effluent.
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spelling pubmed-46403662015-11-11 Mussel processing wastewater: a low-cost substrate for the production of astaxanthin by Xanthophyllomyces dendrorhous Amado, Isabel Rodríguez Vázquez, José Antonio Microb Cell Fact Research BACKGROUND: The use of astaxanthin in different industries such as the chemical, pharmaceutical, food, animal feed and cosmetic has been receiving increasing attention in recent years. Natural supplies of the pigment include crustacean by-products, algal, and microbial cultivation, being the yeast Xanthophyllomyces dendrorhous together with the alga Haematococcus pluvialis the most promising microorganisms for this bioproduction. Different vegetable by-products of the food industry have been explored so far as low-cost substrates for the production of astaxanthin by X. dendrorhous. This study focuses for the first time on the use of a low-cost formulated medium from a marine by-product, mussel-processing wastewater, for the production of astaxanthin by the yeast X. dendrorhous. RESULTS: The yeast was able to grow in non-saccharified mussel broth, revealing the ability of the microorganism to hydrolyze glycogen. However, partial glycogen saccharification with α-amylase was needed for astaxanthin biosynthesis, obtaining maximal productions of 22.5–26.0 mg/L towards the end of the culture and coinciding with yeast highest amylolytic activity. Cultivations in totally-saccharified media revealed an increase in maximal cell concentrations and a decrease in maximal growth rates and astaxanthin production with increasing glucose initial concentration. CONCLUSIONS: Astaxanthin production was higher in partially-saccharified mussel-processing waste than in synthetic medium (yeast peptone dextrose) containing glucose as carbon source (13 mg/L), suggesting this by-product is a promising nutritive medium for astaxanthin production. The use of this effluent also contributes towards the recycling and depuration of this highly pollutant effluent. BioMed Central 2015-11-09 /pmc/articles/PMC4640366/ /pubmed/26553283 http://dx.doi.org/10.1186/s12934-015-0375-5 Text en © Amado and Vázquez. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Amado, Isabel Rodríguez
Vázquez, José Antonio
Mussel processing wastewater: a low-cost substrate for the production of astaxanthin by Xanthophyllomyces dendrorhous
title Mussel processing wastewater: a low-cost substrate for the production of astaxanthin by Xanthophyllomyces dendrorhous
title_full Mussel processing wastewater: a low-cost substrate for the production of astaxanthin by Xanthophyllomyces dendrorhous
title_fullStr Mussel processing wastewater: a low-cost substrate for the production of astaxanthin by Xanthophyllomyces dendrorhous
title_full_unstemmed Mussel processing wastewater: a low-cost substrate for the production of astaxanthin by Xanthophyllomyces dendrorhous
title_short Mussel processing wastewater: a low-cost substrate for the production of astaxanthin by Xanthophyllomyces dendrorhous
title_sort mussel processing wastewater: a low-cost substrate for the production of astaxanthin by xanthophyllomyces dendrorhous
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640366/
https://www.ncbi.nlm.nih.gov/pubmed/26553283
http://dx.doi.org/10.1186/s12934-015-0375-5
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