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Pop in, pop out: a novel gene-targeting strategy for use with CRISPR-Cas9
The CRISPR-Cas9 system is frequently used to create small deletions in the genomes of mammalian cells, but the isolation of precisely targeted mutants is still challenging. A new, two-step ‘pop in & out’ targeting approach facilitates this task. See related Research article: http://www.genomebio...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640370/ https://www.ncbi.nlm.nih.gov/pubmed/26553112 http://dx.doi.org/10.1186/s13059-015-0810-2 |
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author | Kühn, Ralf Chu, Van Trung |
author_facet | Kühn, Ralf Chu, Van Trung |
author_sort | Kühn, Ralf |
collection | PubMed |
description | The CRISPR-Cas9 system is frequently used to create small deletions in the genomes of mammalian cells, but the isolation of precisely targeted mutants is still challenging. A new, two-step ‘pop in & out’ targeting approach facilitates this task. See related Research article: http://www.genomebiology.com/2015/16/1/231 |
format | Online Article Text |
id | pubmed-4640370 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-46403702015-11-11 Pop in, pop out: a novel gene-targeting strategy for use with CRISPR-Cas9 Kühn, Ralf Chu, Van Trung Genome Biol Research Highlight The CRISPR-Cas9 system is frequently used to create small deletions in the genomes of mammalian cells, but the isolation of precisely targeted mutants is still challenging. A new, two-step ‘pop in & out’ targeting approach facilitates this task. See related Research article: http://www.genomebiology.com/2015/16/1/231 BioMed Central 2015-11-10 2015 /pmc/articles/PMC4640370/ /pubmed/26553112 http://dx.doi.org/10.1186/s13059-015-0810-2 Text en © Kühn and Chu. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Highlight Kühn, Ralf Chu, Van Trung Pop in, pop out: a novel gene-targeting strategy for use with CRISPR-Cas9 |
title | Pop in, pop out: a novel gene-targeting strategy for use with CRISPR-Cas9 |
title_full | Pop in, pop out: a novel gene-targeting strategy for use with CRISPR-Cas9 |
title_fullStr | Pop in, pop out: a novel gene-targeting strategy for use with CRISPR-Cas9 |
title_full_unstemmed | Pop in, pop out: a novel gene-targeting strategy for use with CRISPR-Cas9 |
title_short | Pop in, pop out: a novel gene-targeting strategy for use with CRISPR-Cas9 |
title_sort | pop in, pop out: a novel gene-targeting strategy for use with crispr-cas9 |
topic | Research Highlight |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640370/ https://www.ncbi.nlm.nih.gov/pubmed/26553112 http://dx.doi.org/10.1186/s13059-015-0810-2 |
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