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Pop in, pop out: a novel gene-targeting strategy for use with CRISPR-Cas9

The CRISPR-Cas9 system is frequently used to create small deletions in the genomes of mammalian cells, but the isolation of precisely targeted mutants is still challenging. A new, two-step ‘pop in & out’ targeting approach facilitates this task. See related Research article: http://www.genomebio...

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Detalles Bibliográficos
Autores principales: Kühn, Ralf, Chu, Van Trung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640370/
https://www.ncbi.nlm.nih.gov/pubmed/26553112
http://dx.doi.org/10.1186/s13059-015-0810-2
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author Kühn, Ralf
Chu, Van Trung
author_facet Kühn, Ralf
Chu, Van Trung
author_sort Kühn, Ralf
collection PubMed
description The CRISPR-Cas9 system is frequently used to create small deletions in the genomes of mammalian cells, but the isolation of precisely targeted mutants is still challenging. A new, two-step ‘pop in & out’ targeting approach facilitates this task. See related Research article: http://www.genomebiology.com/2015/16/1/231
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spelling pubmed-46403702015-11-11 Pop in, pop out: a novel gene-targeting strategy for use with CRISPR-Cas9 Kühn, Ralf Chu, Van Trung Genome Biol Research Highlight The CRISPR-Cas9 system is frequently used to create small deletions in the genomes of mammalian cells, but the isolation of precisely targeted mutants is still challenging. A new, two-step ‘pop in & out’ targeting approach facilitates this task. See related Research article: http://www.genomebiology.com/2015/16/1/231 BioMed Central 2015-11-10 2015 /pmc/articles/PMC4640370/ /pubmed/26553112 http://dx.doi.org/10.1186/s13059-015-0810-2 Text en © Kühn and Chu. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Highlight
Kühn, Ralf
Chu, Van Trung
Pop in, pop out: a novel gene-targeting strategy for use with CRISPR-Cas9
title Pop in, pop out: a novel gene-targeting strategy for use with CRISPR-Cas9
title_full Pop in, pop out: a novel gene-targeting strategy for use with CRISPR-Cas9
title_fullStr Pop in, pop out: a novel gene-targeting strategy for use with CRISPR-Cas9
title_full_unstemmed Pop in, pop out: a novel gene-targeting strategy for use with CRISPR-Cas9
title_short Pop in, pop out: a novel gene-targeting strategy for use with CRISPR-Cas9
title_sort pop in, pop out: a novel gene-targeting strategy for use with crispr-cas9
topic Research Highlight
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640370/
https://www.ncbi.nlm.nih.gov/pubmed/26553112
http://dx.doi.org/10.1186/s13059-015-0810-2
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