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A Temporal Window for Signal Activation Dictates the Dimensions of a Nodal Signaling Domain

Morphogen signaling is critical for the growth and patterning of tissues in embryos and adults, but how morphogen signaling gradients are generated in tissues remains controversial. The morphogen Nodal was proposed to form a long-range signaling gradient via a reaction-diffusion system, on the basis...

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Detalles Bibliográficos
Autores principales: van Boxtel, Antonius L., Chesebro, John E., Heliot, Claire, Ramel, Marie-Christine, Stone, Richard K., Hill, Caroline S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640439/
https://www.ncbi.nlm.nih.gov/pubmed/26506307
http://dx.doi.org/10.1016/j.devcel.2015.09.014
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author van Boxtel, Antonius L.
Chesebro, John E.
Heliot, Claire
Ramel, Marie-Christine
Stone, Richard K.
Hill, Caroline S.
author_facet van Boxtel, Antonius L.
Chesebro, John E.
Heliot, Claire
Ramel, Marie-Christine
Stone, Richard K.
Hill, Caroline S.
author_sort van Boxtel, Antonius L.
collection PubMed
description Morphogen signaling is critical for the growth and patterning of tissues in embryos and adults, but how morphogen signaling gradients are generated in tissues remains controversial. The morphogen Nodal was proposed to form a long-range signaling gradient via a reaction-diffusion system, on the basis of differential diffusion rates of Nodal and its antagonist Lefty. Here we use a specific zebrafish Nodal biosensor combined with immunofluorescence for phosphorylated Smad2 to demonstrate that endogenous Nodal is unlikely to diffuse over a long range. Instead, short-range Nodal signaling activation in a temporal window is sufficient to determine the dimensions of the Nodal signaling domain. The size of this temporal window is set by the differentially timed production of Nodal and Lefty, which arises mainly from repression of Lefty translation by the microRNA miR-430. Thus, temporal information is transformed into spatial information to define the dimensions of the Nodal signaling domain and, consequently, to specify mesendoderm.
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spelling pubmed-46404392015-12-03 A Temporal Window for Signal Activation Dictates the Dimensions of a Nodal Signaling Domain van Boxtel, Antonius L. Chesebro, John E. Heliot, Claire Ramel, Marie-Christine Stone, Richard K. Hill, Caroline S. Dev Cell Article Morphogen signaling is critical for the growth and patterning of tissues in embryos and adults, but how morphogen signaling gradients are generated in tissues remains controversial. The morphogen Nodal was proposed to form a long-range signaling gradient via a reaction-diffusion system, on the basis of differential diffusion rates of Nodal and its antagonist Lefty. Here we use a specific zebrafish Nodal biosensor combined with immunofluorescence for phosphorylated Smad2 to demonstrate that endogenous Nodal is unlikely to diffuse over a long range. Instead, short-range Nodal signaling activation in a temporal window is sufficient to determine the dimensions of the Nodal signaling domain. The size of this temporal window is set by the differentially timed production of Nodal and Lefty, which arises mainly from repression of Lefty translation by the microRNA miR-430. Thus, temporal information is transformed into spatial information to define the dimensions of the Nodal signaling domain and, consequently, to specify mesendoderm. Cell Press 2015-10-26 /pmc/articles/PMC4640439/ /pubmed/26506307 http://dx.doi.org/10.1016/j.devcel.2015.09.014 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
van Boxtel, Antonius L.
Chesebro, John E.
Heliot, Claire
Ramel, Marie-Christine
Stone, Richard K.
Hill, Caroline S.
A Temporal Window for Signal Activation Dictates the Dimensions of a Nodal Signaling Domain
title A Temporal Window for Signal Activation Dictates the Dimensions of a Nodal Signaling Domain
title_full A Temporal Window for Signal Activation Dictates the Dimensions of a Nodal Signaling Domain
title_fullStr A Temporal Window for Signal Activation Dictates the Dimensions of a Nodal Signaling Domain
title_full_unstemmed A Temporal Window for Signal Activation Dictates the Dimensions of a Nodal Signaling Domain
title_short A Temporal Window for Signal Activation Dictates the Dimensions of a Nodal Signaling Domain
title_sort temporal window for signal activation dictates the dimensions of a nodal signaling domain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640439/
https://www.ncbi.nlm.nih.gov/pubmed/26506307
http://dx.doi.org/10.1016/j.devcel.2015.09.014
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