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Enhancer of Acetyltransferase Chameau (EAChm) Is a Novel Transcriptional Co-Activator
Acetylation of nucleosomal histones by diverse histone acetyltransferases (HAT) plays pivotal roles in many cellular events. Discoveries of novel HATs and HAT related factors have provided new insights to understand the roles and mechanisms of histone acetylation. In this study, we identified promin...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640846/ https://www.ncbi.nlm.nih.gov/pubmed/26555228 http://dx.doi.org/10.1371/journal.pone.0142305 |
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author | Nakagawa, Takeya Ikehara, Tsuyoshi Doiguchi, Masamichi Imamura, Yuko Higashi, Miki Yoneda, Mitsuhiro Ito, Takashi |
author_facet | Nakagawa, Takeya Ikehara, Tsuyoshi Doiguchi, Masamichi Imamura, Yuko Higashi, Miki Yoneda, Mitsuhiro Ito, Takashi |
author_sort | Nakagawa, Takeya |
collection | PubMed |
description | Acetylation of nucleosomal histones by diverse histone acetyltransferases (HAT) plays pivotal roles in many cellular events. Discoveries of novel HATs and HAT related factors have provided new insights to understand the roles and mechanisms of histone acetylation. In this study, we identified prominent Histone H3 acetylation activity in vitro and purified its activity, showing that it is composed of the MYST acetyltransferase Chameau and Enhancer of the Acetyltransferase Chameau (EAChm) family. EAChm is a negatively charged acidic protein retaining aspartate and glutamate. Furthermore, we identified that Chameau and EAChm stimulate transcription in vitro together with purified general transcription factors. In addition, RNA-seq analysis of Chameu KD and EAChm KD S2 cells suggest that Chameau and EAChm regulate transcription of common genes in vivo. Our results suggest that EAChm regulates gene transcription in Drosophila embryos by enhancing Acetyltransferase Chameau activity. |
format | Online Article Text |
id | pubmed-4640846 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-46408462015-11-13 Enhancer of Acetyltransferase Chameau (EAChm) Is a Novel Transcriptional Co-Activator Nakagawa, Takeya Ikehara, Tsuyoshi Doiguchi, Masamichi Imamura, Yuko Higashi, Miki Yoneda, Mitsuhiro Ito, Takashi PLoS One Research Article Acetylation of nucleosomal histones by diverse histone acetyltransferases (HAT) plays pivotal roles in many cellular events. Discoveries of novel HATs and HAT related factors have provided new insights to understand the roles and mechanisms of histone acetylation. In this study, we identified prominent Histone H3 acetylation activity in vitro and purified its activity, showing that it is composed of the MYST acetyltransferase Chameau and Enhancer of the Acetyltransferase Chameau (EAChm) family. EAChm is a negatively charged acidic protein retaining aspartate and glutamate. Furthermore, we identified that Chameau and EAChm stimulate transcription in vitro together with purified general transcription factors. In addition, RNA-seq analysis of Chameu KD and EAChm KD S2 cells suggest that Chameau and EAChm regulate transcription of common genes in vivo. Our results suggest that EAChm regulates gene transcription in Drosophila embryos by enhancing Acetyltransferase Chameau activity. Public Library of Science 2015-11-10 /pmc/articles/PMC4640846/ /pubmed/26555228 http://dx.doi.org/10.1371/journal.pone.0142305 Text en © 2015 Nakagawa et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Nakagawa, Takeya Ikehara, Tsuyoshi Doiguchi, Masamichi Imamura, Yuko Higashi, Miki Yoneda, Mitsuhiro Ito, Takashi Enhancer of Acetyltransferase Chameau (EAChm) Is a Novel Transcriptional Co-Activator |
title | Enhancer of Acetyltransferase Chameau (EAChm) Is a Novel Transcriptional Co-Activator |
title_full | Enhancer of Acetyltransferase Chameau (EAChm) Is a Novel Transcriptional Co-Activator |
title_fullStr | Enhancer of Acetyltransferase Chameau (EAChm) Is a Novel Transcriptional Co-Activator |
title_full_unstemmed | Enhancer of Acetyltransferase Chameau (EAChm) Is a Novel Transcriptional Co-Activator |
title_short | Enhancer of Acetyltransferase Chameau (EAChm) Is a Novel Transcriptional Co-Activator |
title_sort | enhancer of acetyltransferase chameau (eachm) is a novel transcriptional co-activator |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640846/ https://www.ncbi.nlm.nih.gov/pubmed/26555228 http://dx.doi.org/10.1371/journal.pone.0142305 |
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