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Enhancer of Acetyltransferase Chameau (EAChm) Is a Novel Transcriptional Co-Activator

Acetylation of nucleosomal histones by diverse histone acetyltransferases (HAT) plays pivotal roles in many cellular events. Discoveries of novel HATs and HAT related factors have provided new insights to understand the roles and mechanisms of histone acetylation. In this study, we identified promin...

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Autores principales: Nakagawa, Takeya, Ikehara, Tsuyoshi, Doiguchi, Masamichi, Imamura, Yuko, Higashi, Miki, Yoneda, Mitsuhiro, Ito, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640846/
https://www.ncbi.nlm.nih.gov/pubmed/26555228
http://dx.doi.org/10.1371/journal.pone.0142305
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author Nakagawa, Takeya
Ikehara, Tsuyoshi
Doiguchi, Masamichi
Imamura, Yuko
Higashi, Miki
Yoneda, Mitsuhiro
Ito, Takashi
author_facet Nakagawa, Takeya
Ikehara, Tsuyoshi
Doiguchi, Masamichi
Imamura, Yuko
Higashi, Miki
Yoneda, Mitsuhiro
Ito, Takashi
author_sort Nakagawa, Takeya
collection PubMed
description Acetylation of nucleosomal histones by diverse histone acetyltransferases (HAT) plays pivotal roles in many cellular events. Discoveries of novel HATs and HAT related factors have provided new insights to understand the roles and mechanisms of histone acetylation. In this study, we identified prominent Histone H3 acetylation activity in vitro and purified its activity, showing that it is composed of the MYST acetyltransferase Chameau and Enhancer of the Acetyltransferase Chameau (EAChm) family. EAChm is a negatively charged acidic protein retaining aspartate and glutamate. Furthermore, we identified that Chameau and EAChm stimulate transcription in vitro together with purified general transcription factors. In addition, RNA-seq analysis of Chameu KD and EAChm KD S2 cells suggest that Chameau and EAChm regulate transcription of common genes in vivo. Our results suggest that EAChm regulates gene transcription in Drosophila embryos by enhancing Acetyltransferase Chameau activity.
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spelling pubmed-46408462015-11-13 Enhancer of Acetyltransferase Chameau (EAChm) Is a Novel Transcriptional Co-Activator Nakagawa, Takeya Ikehara, Tsuyoshi Doiguchi, Masamichi Imamura, Yuko Higashi, Miki Yoneda, Mitsuhiro Ito, Takashi PLoS One Research Article Acetylation of nucleosomal histones by diverse histone acetyltransferases (HAT) plays pivotal roles in many cellular events. Discoveries of novel HATs and HAT related factors have provided new insights to understand the roles and mechanisms of histone acetylation. In this study, we identified prominent Histone H3 acetylation activity in vitro and purified its activity, showing that it is composed of the MYST acetyltransferase Chameau and Enhancer of the Acetyltransferase Chameau (EAChm) family. EAChm is a negatively charged acidic protein retaining aspartate and glutamate. Furthermore, we identified that Chameau and EAChm stimulate transcription in vitro together with purified general transcription factors. In addition, RNA-seq analysis of Chameu KD and EAChm KD S2 cells suggest that Chameau and EAChm regulate transcription of common genes in vivo. Our results suggest that EAChm regulates gene transcription in Drosophila embryos by enhancing Acetyltransferase Chameau activity. Public Library of Science 2015-11-10 /pmc/articles/PMC4640846/ /pubmed/26555228 http://dx.doi.org/10.1371/journal.pone.0142305 Text en © 2015 Nakagawa et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Nakagawa, Takeya
Ikehara, Tsuyoshi
Doiguchi, Masamichi
Imamura, Yuko
Higashi, Miki
Yoneda, Mitsuhiro
Ito, Takashi
Enhancer of Acetyltransferase Chameau (EAChm) Is a Novel Transcriptional Co-Activator
title Enhancer of Acetyltransferase Chameau (EAChm) Is a Novel Transcriptional Co-Activator
title_full Enhancer of Acetyltransferase Chameau (EAChm) Is a Novel Transcriptional Co-Activator
title_fullStr Enhancer of Acetyltransferase Chameau (EAChm) Is a Novel Transcriptional Co-Activator
title_full_unstemmed Enhancer of Acetyltransferase Chameau (EAChm) Is a Novel Transcriptional Co-Activator
title_short Enhancer of Acetyltransferase Chameau (EAChm) Is a Novel Transcriptional Co-Activator
title_sort enhancer of acetyltransferase chameau (eachm) is a novel transcriptional co-activator
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4640846/
https://www.ncbi.nlm.nih.gov/pubmed/26555228
http://dx.doi.org/10.1371/journal.pone.0142305
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