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Characterization of Leptospira interrogans Serovars by Polymorphism Variable Number Tandem Repeat Analysis
BACKGROUND: Leptospirosis is recognized as a re-emerging infectious disease; therefore, understanding the epidemiology of the disease is vital for designing intervention programs and diminishing its transmission. Recently, Multilocus variable number tandem repeat analysis (MLVA) is used for segregat...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Kowsar
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4641467/ https://www.ncbi.nlm.nih.gov/pubmed/26568805 http://dx.doi.org/10.5812/jjm.22819 |
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author | Rezasoltani, Sama Dabiri, Hossein Khaki, Pejvak Rostami Nejad, Mohammad Karimnasab, Nasim Modirrousta, Shiva |
author_facet | Rezasoltani, Sama Dabiri, Hossein Khaki, Pejvak Rostami Nejad, Mohammad Karimnasab, Nasim Modirrousta, Shiva |
author_sort | Rezasoltani, Sama |
collection | PubMed |
description | BACKGROUND: Leptospirosis is recognized as a re-emerging infectious disease; therefore, understanding the epidemiology of the disease is vital for designing intervention programs and diminishing its transmission. Recently, Multilocus variable number tandem repeat analysis (MLVA) is used for segregating and identifying Leptospira serovars. The method has potential application in investigating the molecular epidemiology of Leptospira. OBJECTIVES: The propose of this study was genomic identification of pathogenic Leptospires in Iran by MLVA. MATERIALS AND METHODS: Leptospira serovars were obtained from National Reference Laboratory of Leptospira at Razi Vaccine and Serum Research Institute, Karaj, Iran. Serovars were cultured into the liquid EMJH medium and incubated at 28˚C for 7 days. DNA of serovars was extracted using the phenol-chloroform method. PCR was performed with 5 selected variable number tandem repeat analysis (VNTR) loci. The amplified products were analyzed by agarose gel electrophoresis. The size of the amplified products was estimated by 100 bp ladder and sequencing analysis. RESULTS: The saprophytic serovar showed no amplified fragments. PCR products in all pathogenic serovars were observed. The 12 reference serovars used for the development of technique displayed distinct patterns. CONCLUSIONS: Results showed that MLVA technique with its range of polymorphism is a good marker for identification of pathogenic serovars. Some VNTR loci are more powerful than the other ones with regard to differentiation. Serovars from the same geographical area have more genetic similarity than same serovars from different places. MLVA is a suitable technique for epidemiological survey. |
format | Online Article Text |
id | pubmed-4641467 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Kowsar |
record_format | MEDLINE/PubMed |
spelling | pubmed-46414672015-11-13 Characterization of Leptospira interrogans Serovars by Polymorphism Variable Number Tandem Repeat Analysis Rezasoltani, Sama Dabiri, Hossein Khaki, Pejvak Rostami Nejad, Mohammad Karimnasab, Nasim Modirrousta, Shiva Jundishapur J Microbiol Research Article BACKGROUND: Leptospirosis is recognized as a re-emerging infectious disease; therefore, understanding the epidemiology of the disease is vital for designing intervention programs and diminishing its transmission. Recently, Multilocus variable number tandem repeat analysis (MLVA) is used for segregating and identifying Leptospira serovars. The method has potential application in investigating the molecular epidemiology of Leptospira. OBJECTIVES: The propose of this study was genomic identification of pathogenic Leptospires in Iran by MLVA. MATERIALS AND METHODS: Leptospira serovars were obtained from National Reference Laboratory of Leptospira at Razi Vaccine and Serum Research Institute, Karaj, Iran. Serovars were cultured into the liquid EMJH medium and incubated at 28˚C for 7 days. DNA of serovars was extracted using the phenol-chloroform method. PCR was performed with 5 selected variable number tandem repeat analysis (VNTR) loci. The amplified products were analyzed by agarose gel electrophoresis. The size of the amplified products was estimated by 100 bp ladder and sequencing analysis. RESULTS: The saprophytic serovar showed no amplified fragments. PCR products in all pathogenic serovars were observed. The 12 reference serovars used for the development of technique displayed distinct patterns. CONCLUSIONS: Results showed that MLVA technique with its range of polymorphism is a good marker for identification of pathogenic serovars. Some VNTR loci are more powerful than the other ones with regard to differentiation. Serovars from the same geographical area have more genetic similarity than same serovars from different places. MLVA is a suitable technique for epidemiological survey. Kowsar 2015-10-18 /pmc/articles/PMC4641467/ /pubmed/26568805 http://dx.doi.org/10.5812/jjm.22819 Text en Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited. |
spellingShingle | Research Article Rezasoltani, Sama Dabiri, Hossein Khaki, Pejvak Rostami Nejad, Mohammad Karimnasab, Nasim Modirrousta, Shiva Characterization of Leptospira interrogans Serovars by Polymorphism Variable Number Tandem Repeat Analysis |
title | Characterization of Leptospira interrogans Serovars by Polymorphism Variable Number Tandem Repeat Analysis |
title_full | Characterization of Leptospira interrogans Serovars by Polymorphism Variable Number Tandem Repeat Analysis |
title_fullStr | Characterization of Leptospira interrogans Serovars by Polymorphism Variable Number Tandem Repeat Analysis |
title_full_unstemmed | Characterization of Leptospira interrogans Serovars by Polymorphism Variable Number Tandem Repeat Analysis |
title_short | Characterization of Leptospira interrogans Serovars by Polymorphism Variable Number Tandem Repeat Analysis |
title_sort | characterization of leptospira interrogans serovars by polymorphism variable number tandem repeat analysis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4641467/ https://www.ncbi.nlm.nih.gov/pubmed/26568805 http://dx.doi.org/10.5812/jjm.22819 |
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