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Triplet–triplet annihilation upconversion followed by FRET for the red light activation of a photodissociative ruthenium complex in liposomes

Upconversion is a promising way to trigger high-energy photochemistry with low-energy photons. However, combining upconversion schemes with non-radiative energy transfer is challenging because bringing several photochemically active components in close proximity results in complex multi-component sy...

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Autores principales: Askes, Sven H. C., Kloz, Miroslav, Bruylants, Gilles, Kennis, John T. M., Bonnet, Sylvestre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4642198/
https://www.ncbi.nlm.nih.gov/pubmed/26420663
http://dx.doi.org/10.1039/c5cp04352b
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author Askes, Sven H. C.
Kloz, Miroslav
Bruylants, Gilles
Kennis, John T. M.
Bonnet, Sylvestre
author_facet Askes, Sven H. C.
Kloz, Miroslav
Bruylants, Gilles
Kennis, John T. M.
Bonnet, Sylvestre
author_sort Askes, Sven H. C.
collection PubMed
description Upconversion is a promising way to trigger high-energy photochemistry with low-energy photons. However, combining upconversion schemes with non-radiative energy transfer is challenging because bringing several photochemically active components in close proximity results in complex multi-component systems where quenching processes may deactivate the whole assembly. In this work, PEGylated liposomes were prepared that contained three photoactive components: a porphyrin dye absorbing red light, a perylene moiety emitting in the blue, and a light-activatable ruthenium prodrug sensitive to blue light. Time-dependent spectroscopic studies demonstrate that singlet perylene excited states are non-radiatively transferred to the nearby ruthenium complex by Förster resonance energy transfer (FRET). Under red-light irradiation of the three-component membranes, triplet–triplet annihilation upconversion (TTA-UC) occurs followed by FRET, which results in a more efficient activation of the ruthenium prodrug compared to a physical mixture of two-component upconverting liposomes and liposomes containing only the ruthenium complex. This work represents a rare example where TTA-UC and Förster resonance energy transfer are combined to achieve prodrug activation in the phototherapeutic window.
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spelling pubmed-46421982015-12-01 Triplet–triplet annihilation upconversion followed by FRET for the red light activation of a photodissociative ruthenium complex in liposomes Askes, Sven H. C. Kloz, Miroslav Bruylants, Gilles Kennis, John T. M. Bonnet, Sylvestre Phys Chem Chem Phys Chemistry Upconversion is a promising way to trigger high-energy photochemistry with low-energy photons. However, combining upconversion schemes with non-radiative energy transfer is challenging because bringing several photochemically active components in close proximity results in complex multi-component systems where quenching processes may deactivate the whole assembly. In this work, PEGylated liposomes were prepared that contained three photoactive components: a porphyrin dye absorbing red light, a perylene moiety emitting in the blue, and a light-activatable ruthenium prodrug sensitive to blue light. Time-dependent spectroscopic studies demonstrate that singlet perylene excited states are non-radiatively transferred to the nearby ruthenium complex by Förster resonance energy transfer (FRET). Under red-light irradiation of the three-component membranes, triplet–triplet annihilation upconversion (TTA-UC) occurs followed by FRET, which results in a more efficient activation of the ruthenium prodrug compared to a physical mixture of two-component upconverting liposomes and liposomes containing only the ruthenium complex. This work represents a rare example where TTA-UC and Förster resonance energy transfer are combined to achieve prodrug activation in the phototherapeutic window. Royal Society of Chemistry 2015-10-14 2015-11-07 /pmc/articles/PMC4642198/ /pubmed/26420663 http://dx.doi.org/10.1039/c5cp04352b Text en This journal is © The Royal Society of Chemistry 2015 http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 Unported License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Askes, Sven H. C.
Kloz, Miroslav
Bruylants, Gilles
Kennis, John T. M.
Bonnet, Sylvestre
Triplet–triplet annihilation upconversion followed by FRET for the red light activation of a photodissociative ruthenium complex in liposomes
title Triplet–triplet annihilation upconversion followed by FRET for the red light activation of a photodissociative ruthenium complex in liposomes
title_full Triplet–triplet annihilation upconversion followed by FRET for the red light activation of a photodissociative ruthenium complex in liposomes
title_fullStr Triplet–triplet annihilation upconversion followed by FRET for the red light activation of a photodissociative ruthenium complex in liposomes
title_full_unstemmed Triplet–triplet annihilation upconversion followed by FRET for the red light activation of a photodissociative ruthenium complex in liposomes
title_short Triplet–triplet annihilation upconversion followed by FRET for the red light activation of a photodissociative ruthenium complex in liposomes
title_sort triplet–triplet annihilation upconversion followed by fret for the red light activation of a photodissociative ruthenium complex in liposomes
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4642198/
https://www.ncbi.nlm.nih.gov/pubmed/26420663
http://dx.doi.org/10.1039/c5cp04352b
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