Cargando…
Triplet–triplet annihilation upconversion followed by FRET for the red light activation of a photodissociative ruthenium complex in liposomes
Upconversion is a promising way to trigger high-energy photochemistry with low-energy photons. However, combining upconversion schemes with non-radiative energy transfer is challenging because bringing several photochemically active components in close proximity results in complex multi-component sy...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royal Society of Chemistry
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4642198/ https://www.ncbi.nlm.nih.gov/pubmed/26420663 http://dx.doi.org/10.1039/c5cp04352b |
_version_ | 1782400325237342208 |
---|---|
author | Askes, Sven H. C. Kloz, Miroslav Bruylants, Gilles Kennis, John T. M. Bonnet, Sylvestre |
author_facet | Askes, Sven H. C. Kloz, Miroslav Bruylants, Gilles Kennis, John T. M. Bonnet, Sylvestre |
author_sort | Askes, Sven H. C. |
collection | PubMed |
description | Upconversion is a promising way to trigger high-energy photochemistry with low-energy photons. However, combining upconversion schemes with non-radiative energy transfer is challenging because bringing several photochemically active components in close proximity results in complex multi-component systems where quenching processes may deactivate the whole assembly. In this work, PEGylated liposomes were prepared that contained three photoactive components: a porphyrin dye absorbing red light, a perylene moiety emitting in the blue, and a light-activatable ruthenium prodrug sensitive to blue light. Time-dependent spectroscopic studies demonstrate that singlet perylene excited states are non-radiatively transferred to the nearby ruthenium complex by Förster resonance energy transfer (FRET). Under red-light irradiation of the three-component membranes, triplet–triplet annihilation upconversion (TTA-UC) occurs followed by FRET, which results in a more efficient activation of the ruthenium prodrug compared to a physical mixture of two-component upconverting liposomes and liposomes containing only the ruthenium complex. This work represents a rare example where TTA-UC and Förster resonance energy transfer are combined to achieve prodrug activation in the phototherapeutic window. |
format | Online Article Text |
id | pubmed-4642198 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-46421982015-12-01 Triplet–triplet annihilation upconversion followed by FRET for the red light activation of a photodissociative ruthenium complex in liposomes Askes, Sven H. C. Kloz, Miroslav Bruylants, Gilles Kennis, John T. M. Bonnet, Sylvestre Phys Chem Chem Phys Chemistry Upconversion is a promising way to trigger high-energy photochemistry with low-energy photons. However, combining upconversion schemes with non-radiative energy transfer is challenging because bringing several photochemically active components in close proximity results in complex multi-component systems where quenching processes may deactivate the whole assembly. In this work, PEGylated liposomes were prepared that contained three photoactive components: a porphyrin dye absorbing red light, a perylene moiety emitting in the blue, and a light-activatable ruthenium prodrug sensitive to blue light. Time-dependent spectroscopic studies demonstrate that singlet perylene excited states are non-radiatively transferred to the nearby ruthenium complex by Förster resonance energy transfer (FRET). Under red-light irradiation of the three-component membranes, triplet–triplet annihilation upconversion (TTA-UC) occurs followed by FRET, which results in a more efficient activation of the ruthenium prodrug compared to a physical mixture of two-component upconverting liposomes and liposomes containing only the ruthenium complex. This work represents a rare example where TTA-UC and Förster resonance energy transfer are combined to achieve prodrug activation in the phototherapeutic window. Royal Society of Chemistry 2015-10-14 2015-11-07 /pmc/articles/PMC4642198/ /pubmed/26420663 http://dx.doi.org/10.1039/c5cp04352b Text en This journal is © The Royal Society of Chemistry 2015 http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 Unported License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Chemistry Askes, Sven H. C. Kloz, Miroslav Bruylants, Gilles Kennis, John T. M. Bonnet, Sylvestre Triplet–triplet annihilation upconversion followed by FRET for the red light activation of a photodissociative ruthenium complex in liposomes |
title | Triplet–triplet annihilation upconversion followed by FRET for the red light activation of a photodissociative ruthenium complex in liposomes
|
title_full | Triplet–triplet annihilation upconversion followed by FRET for the red light activation of a photodissociative ruthenium complex in liposomes
|
title_fullStr | Triplet–triplet annihilation upconversion followed by FRET for the red light activation of a photodissociative ruthenium complex in liposomes
|
title_full_unstemmed | Triplet–triplet annihilation upconversion followed by FRET for the red light activation of a photodissociative ruthenium complex in liposomes
|
title_short | Triplet–triplet annihilation upconversion followed by FRET for the red light activation of a photodissociative ruthenium complex in liposomes
|
title_sort | triplet–triplet annihilation upconversion followed by fret for the red light activation of a photodissociative ruthenium complex in liposomes |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4642198/ https://www.ncbi.nlm.nih.gov/pubmed/26420663 http://dx.doi.org/10.1039/c5cp04352b |
work_keys_str_mv | AT askessvenhc triplettripletannihilationupconversionfollowedbyfretfortheredlightactivationofaphotodissociativerutheniumcomplexinliposomes AT klozmiroslav triplettripletannihilationupconversionfollowedbyfretfortheredlightactivationofaphotodissociativerutheniumcomplexinliposomes AT bruylantsgilles triplettripletannihilationupconversionfollowedbyfretfortheredlightactivationofaphotodissociativerutheniumcomplexinliposomes AT kennisjohntm triplettripletannihilationupconversionfollowedbyfretfortheredlightactivationofaphotodissociativerutheniumcomplexinliposomes AT bonnetsylvestre triplettripletannihilationupconversionfollowedbyfretfortheredlightactivationofaphotodissociativerutheniumcomplexinliposomes |