Cargando…
Paths and determinants for Penicillium janthinellum to resist low and high copper
Copper (Cu) tolerance was well understood in fungi yeasts but not in filamentous fungi. Filamentous fungi are eukaryotes but unlike eukaryotic fungi yeasts, which are a collection of various fungi that are maybe classified into different taxa but all characterized by growth as filamentous hyphae cel...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4642507/ https://www.ncbi.nlm.nih.gov/pubmed/26265593 http://dx.doi.org/10.1038/srep10590 |
_version_ | 1782400367662727168 |
---|---|
author | Xu, Jian Chen, Guo-Li Sun, Xue-Zhe Fan, Xian-Wei You-Zhi, Li |
author_facet | Xu, Jian Chen, Guo-Li Sun, Xue-Zhe Fan, Xian-Wei You-Zhi, Li |
author_sort | Xu, Jian |
collection | PubMed |
description | Copper (Cu) tolerance was well understood in fungi yeasts but not in filamentous fungi. Filamentous fungi are eukaryotes but unlike eukaryotic fungi yeasts, which are a collection of various fungi that are maybe classified into different taxa but all characterized by growth as filamentous hyphae cells and with a complex morphology. The current knowledge of Cu resistance of filamentous fungi is still fragmental and therefore needs to be bridged. In this study, we characterized Cu resistance of Penicillium janthinellum strain GXCR and its Cu-resistance-decreasing mutants (EC-6 and UC-8), and conducted sequencing of a total of 6 transcriptomes from wild-type GXCR and mutant EC-6 grown under control and external Cu. Taken all the results together, Cu effects on the basal metabolism were directed to solute transport by two superfamilies of solute carrier and major facilitator, the buffering free CoA and Acyl-CoA pool in the peroxisome, F-type H(+)-transporting ATPases-based ATP production, V-type H(+)-transporting ATPases-based transmembrane transport, protein degradation, and alternative splicing of pre-mRNAs. Roles of enzymatic and non-enzymatic antioxidants in resistance to low and high Cu were defined. The backbone paths, signaling systems, and determinants that involve resistance of filamentous fungi to high Cu were determined, discussed and outlined in a model. |
format | Online Article Text |
id | pubmed-4642507 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-46425072015-11-20 Paths and determinants for Penicillium janthinellum to resist low and high copper Xu, Jian Chen, Guo-Li Sun, Xue-Zhe Fan, Xian-Wei You-Zhi, Li Sci Rep Article Copper (Cu) tolerance was well understood in fungi yeasts but not in filamentous fungi. Filamentous fungi are eukaryotes but unlike eukaryotic fungi yeasts, which are a collection of various fungi that are maybe classified into different taxa but all characterized by growth as filamentous hyphae cells and with a complex morphology. The current knowledge of Cu resistance of filamentous fungi is still fragmental and therefore needs to be bridged. In this study, we characterized Cu resistance of Penicillium janthinellum strain GXCR and its Cu-resistance-decreasing mutants (EC-6 and UC-8), and conducted sequencing of a total of 6 transcriptomes from wild-type GXCR and mutant EC-6 grown under control and external Cu. Taken all the results together, Cu effects on the basal metabolism were directed to solute transport by two superfamilies of solute carrier and major facilitator, the buffering free CoA and Acyl-CoA pool in the peroxisome, F-type H(+)-transporting ATPases-based ATP production, V-type H(+)-transporting ATPases-based transmembrane transport, protein degradation, and alternative splicing of pre-mRNAs. Roles of enzymatic and non-enzymatic antioxidants in resistance to low and high Cu were defined. The backbone paths, signaling systems, and determinants that involve resistance of filamentous fungi to high Cu were determined, discussed and outlined in a model. Nature Publishing Group 2015-08-12 /pmc/articles/PMC4642507/ /pubmed/26265593 http://dx.doi.org/10.1038/srep10590 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Xu, Jian Chen, Guo-Li Sun, Xue-Zhe Fan, Xian-Wei You-Zhi, Li Paths and determinants for Penicillium janthinellum to resist low and high copper |
title | Paths and determinants for Penicillium janthinellum to resist low and high copper |
title_full | Paths and determinants for Penicillium janthinellum to resist low and high copper |
title_fullStr | Paths and determinants for Penicillium janthinellum to resist low and high copper |
title_full_unstemmed | Paths and determinants for Penicillium janthinellum to resist low and high copper |
title_short | Paths and determinants for Penicillium janthinellum to resist low and high copper |
title_sort | paths and determinants for penicillium janthinellum to resist low and high copper |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4642507/ https://www.ncbi.nlm.nih.gov/pubmed/26265593 http://dx.doi.org/10.1038/srep10590 |
work_keys_str_mv | AT xujian pathsanddeterminantsforpenicilliumjanthinellumtoresistlowandhighcopper AT chenguoli pathsanddeterminantsforpenicilliumjanthinellumtoresistlowandhighcopper AT sunxuezhe pathsanddeterminantsforpenicilliumjanthinellumtoresistlowandhighcopper AT fanxianwei pathsanddeterminantsforpenicilliumjanthinellumtoresistlowandhighcopper AT youzhili pathsanddeterminantsforpenicilliumjanthinellumtoresistlowandhighcopper |