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Marine amoebae with cytoplasmic and perinuclear symbionts deeply branching in the Gammaproteobacteria
Amoebae play an important ecological role as predators in microbial communities. They also serve as niche for bacterial replication, harbor endosymbiotic bacteria and have contributed to the evolution of major human pathogens. Despite their high diversity, marine amoebae and their association with b...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4642509/ https://www.ncbi.nlm.nih.gov/pubmed/26303516 http://dx.doi.org/10.1038/srep13381 |
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author | Schulz, Frederik Tyml, Tomáš Pizzetti, Ilaria Dyková, Iva Fazi, Stefano Kostka, Martin Horn, Matthias |
author_facet | Schulz, Frederik Tyml, Tomáš Pizzetti, Ilaria Dyková, Iva Fazi, Stefano Kostka, Martin Horn, Matthias |
author_sort | Schulz, Frederik |
collection | PubMed |
description | Amoebae play an important ecological role as predators in microbial communities. They also serve as niche for bacterial replication, harbor endosymbiotic bacteria and have contributed to the evolution of major human pathogens. Despite their high diversity, marine amoebae and their association with bacteria are poorly understood. Here we describe the isolation and characterization of two novel marine amoebae together with their bacterial endosymbionts, tentatively named ‘Candidatus Occultobacter vannellae’ and ‘Candidatus Nucleophilum amoebae’. While one amoeba strain is related to Vannella, a genus common in marine habitats, the other represents a novel lineage in the Amoebozoa. The endosymbionts showed only low similarity to known bacteria (85–88% 16S rRNA sequence similarity) but together with other uncultured marine bacteria form a sister clade to the Coxiellaceae. Using fluorescence in situ hybridization and transmission electron microscopy, identity and intracellular location of both symbionts were confirmed; one was replicating in host-derived vacuoles, whereas the other was located in the perinuclear space of its amoeba host. This study sheds for the first time light on a so far neglected group of protists and their bacterial symbionts. The newly isolated strains represent easily maintainable model systems and pave the way for further studies on marine associations between amoebae and bacterial symbionts. |
format | Online Article Text |
id | pubmed-4642509 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-46425092015-11-20 Marine amoebae with cytoplasmic and perinuclear symbionts deeply branching in the Gammaproteobacteria Schulz, Frederik Tyml, Tomáš Pizzetti, Ilaria Dyková, Iva Fazi, Stefano Kostka, Martin Horn, Matthias Sci Rep Article Amoebae play an important ecological role as predators in microbial communities. They also serve as niche for bacterial replication, harbor endosymbiotic bacteria and have contributed to the evolution of major human pathogens. Despite their high diversity, marine amoebae and their association with bacteria are poorly understood. Here we describe the isolation and characterization of two novel marine amoebae together with their bacterial endosymbionts, tentatively named ‘Candidatus Occultobacter vannellae’ and ‘Candidatus Nucleophilum amoebae’. While one amoeba strain is related to Vannella, a genus common in marine habitats, the other represents a novel lineage in the Amoebozoa. The endosymbionts showed only low similarity to known bacteria (85–88% 16S rRNA sequence similarity) but together with other uncultured marine bacteria form a sister clade to the Coxiellaceae. Using fluorescence in situ hybridization and transmission electron microscopy, identity and intracellular location of both symbionts were confirmed; one was replicating in host-derived vacuoles, whereas the other was located in the perinuclear space of its amoeba host. This study sheds for the first time light on a so far neglected group of protists and their bacterial symbionts. The newly isolated strains represent easily maintainable model systems and pave the way for further studies on marine associations between amoebae and bacterial symbionts. Nature Publishing Group 2015-08-25 /pmc/articles/PMC4642509/ /pubmed/26303516 http://dx.doi.org/10.1038/srep13381 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Schulz, Frederik Tyml, Tomáš Pizzetti, Ilaria Dyková, Iva Fazi, Stefano Kostka, Martin Horn, Matthias Marine amoebae with cytoplasmic and perinuclear symbionts deeply branching in the Gammaproteobacteria |
title | Marine amoebae with cytoplasmic and perinuclear symbionts deeply branching in the Gammaproteobacteria |
title_full | Marine amoebae with cytoplasmic and perinuclear symbionts deeply branching in the Gammaproteobacteria |
title_fullStr | Marine amoebae with cytoplasmic and perinuclear symbionts deeply branching in the Gammaproteobacteria |
title_full_unstemmed | Marine amoebae with cytoplasmic and perinuclear symbionts deeply branching in the Gammaproteobacteria |
title_short | Marine amoebae with cytoplasmic and perinuclear symbionts deeply branching in the Gammaproteobacteria |
title_sort | marine amoebae with cytoplasmic and perinuclear symbionts deeply branching in the gammaproteobacteria |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4642509/ https://www.ncbi.nlm.nih.gov/pubmed/26303516 http://dx.doi.org/10.1038/srep13381 |
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