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A “footprint” of plant carbon fixation cycle functions during the development of a heterotrophic fungus

Carbon fixation pathway of plants (CFPP) in photosynthesis converts solar energy to biomass, bio-products and biofuel. Intriguingly, a large number of heterotrophic fungi also possess enzymes functionally associated with CFPP, raising the questions about their roles in fungal development and in evol...

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Autores principales: Lyu, Xueliang, Shen, Cuicui, Xie, Jiatao, Fu, Yanping, Jiang, Daohong, Hu, Zijin, Tang, Lihua, Tang, Liguang, Ding, Feng, Li, Kunfei, Wu, Song, Hu, Yanping, Luo, Lilian, Li, Yuanhao, Wang, Qihua, Li, Guoqing, Cheng, Jiasen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4642529/
https://www.ncbi.nlm.nih.gov/pubmed/26263551
http://dx.doi.org/10.1038/srep12952
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author Lyu, Xueliang
Shen, Cuicui
Xie, Jiatao
Fu, Yanping
Jiang, Daohong
Hu, Zijin
Tang, Lihua
Tang, Liguang
Ding, Feng
Li, Kunfei
Wu, Song
Hu, Yanping
Luo, Lilian
Li, Yuanhao
Wang, Qihua
Li, Guoqing
Cheng, Jiasen
author_facet Lyu, Xueliang
Shen, Cuicui
Xie, Jiatao
Fu, Yanping
Jiang, Daohong
Hu, Zijin
Tang, Lihua
Tang, Liguang
Ding, Feng
Li, Kunfei
Wu, Song
Hu, Yanping
Luo, Lilian
Li, Yuanhao
Wang, Qihua
Li, Guoqing
Cheng, Jiasen
author_sort Lyu, Xueliang
collection PubMed
description Carbon fixation pathway of plants (CFPP) in photosynthesis converts solar energy to biomass, bio-products and biofuel. Intriguingly, a large number of heterotrophic fungi also possess enzymes functionally associated with CFPP, raising the questions about their roles in fungal development and in evolution. Here, we report on the presence of 17 CFPP associated enzymes (ten in Calvin-Benson-Basham reductive pentose phosphate pathway and seven in C4-dicarboxylic acid cycle) in the genome of Sclerotinia sclerotiorum, a heterotrophic phytopathogenic fungus, and only two unique enzymes: ribulose-1, 5-bisphosphate carboxylase-oxygenase (Rubisco) and phosphoribulokinase (PRK) were absent. This data suggested an incomplete CFPP-like pathway (CLP) in fungi. Functional profile analysis demonstrated that the activity of the incomplete CLP was dramatically regulated during different developmental stages of S. sclerotiorum. Subsequent experiments confirmed that many of them were essential to the virulence and/or sclerotial formation. Most of the CLP associated genes are conserved in fungi. Phylogenetic analysis showed that many of them have undergone gene duplication, gene acquisition or loss and functional diversification in evolutionary history. These findings showed an evolutionary links in the carbon fixation processes of autotrophs and heterotrophs and implicated the functions of related genes were in course of continuous change in different organisms in evolution.
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spelling pubmed-46425292015-11-20 A “footprint” of plant carbon fixation cycle functions during the development of a heterotrophic fungus Lyu, Xueliang Shen, Cuicui Xie, Jiatao Fu, Yanping Jiang, Daohong Hu, Zijin Tang, Lihua Tang, Liguang Ding, Feng Li, Kunfei Wu, Song Hu, Yanping Luo, Lilian Li, Yuanhao Wang, Qihua Li, Guoqing Cheng, Jiasen Sci Rep Article Carbon fixation pathway of plants (CFPP) in photosynthesis converts solar energy to biomass, bio-products and biofuel. Intriguingly, a large number of heterotrophic fungi also possess enzymes functionally associated with CFPP, raising the questions about their roles in fungal development and in evolution. Here, we report on the presence of 17 CFPP associated enzymes (ten in Calvin-Benson-Basham reductive pentose phosphate pathway and seven in C4-dicarboxylic acid cycle) in the genome of Sclerotinia sclerotiorum, a heterotrophic phytopathogenic fungus, and only two unique enzymes: ribulose-1, 5-bisphosphate carboxylase-oxygenase (Rubisco) and phosphoribulokinase (PRK) were absent. This data suggested an incomplete CFPP-like pathway (CLP) in fungi. Functional profile analysis demonstrated that the activity of the incomplete CLP was dramatically regulated during different developmental stages of S. sclerotiorum. Subsequent experiments confirmed that many of them were essential to the virulence and/or sclerotial formation. Most of the CLP associated genes are conserved in fungi. Phylogenetic analysis showed that many of them have undergone gene duplication, gene acquisition or loss and functional diversification in evolutionary history. These findings showed an evolutionary links in the carbon fixation processes of autotrophs and heterotrophs and implicated the functions of related genes were in course of continuous change in different organisms in evolution. Nature Publishing Group 2015-08-11 /pmc/articles/PMC4642529/ /pubmed/26263551 http://dx.doi.org/10.1038/srep12952 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Lyu, Xueliang
Shen, Cuicui
Xie, Jiatao
Fu, Yanping
Jiang, Daohong
Hu, Zijin
Tang, Lihua
Tang, Liguang
Ding, Feng
Li, Kunfei
Wu, Song
Hu, Yanping
Luo, Lilian
Li, Yuanhao
Wang, Qihua
Li, Guoqing
Cheng, Jiasen
A “footprint” of plant carbon fixation cycle functions during the development of a heterotrophic fungus
title A “footprint” of plant carbon fixation cycle functions during the development of a heterotrophic fungus
title_full A “footprint” of plant carbon fixation cycle functions during the development of a heterotrophic fungus
title_fullStr A “footprint” of plant carbon fixation cycle functions during the development of a heterotrophic fungus
title_full_unstemmed A “footprint” of plant carbon fixation cycle functions during the development of a heterotrophic fungus
title_short A “footprint” of plant carbon fixation cycle functions during the development of a heterotrophic fungus
title_sort “footprint” of plant carbon fixation cycle functions during the development of a heterotrophic fungus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4642529/
https://www.ncbi.nlm.nih.gov/pubmed/26263551
http://dx.doi.org/10.1038/srep12952
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