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Embryo-lethal phenotypes in early abp1 mutants are due to disruption of the neighboring BSM gene

The Auxin Binding Protein1 (ABP1) has been identified based on its ability to bind auxin with high affinity and studied for a long time as a prime candidate for the extracellular auxin receptor responsible for mediating in particular the fast non-transcriptional auxin responses. However, the contrad...

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Autores principales: Michalko, Jaroslav, Dravecká, Marta, Bollenbach, Tobias, Friml, Jiří
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000Research 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4642851/
https://www.ncbi.nlm.nih.gov/pubmed/26629335
http://dx.doi.org/10.12688/f1000research.7143.1
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author Michalko, Jaroslav
Dravecká, Marta
Bollenbach, Tobias
Friml, Jiří
author_facet Michalko, Jaroslav
Dravecká, Marta
Bollenbach, Tobias
Friml, Jiří
author_sort Michalko, Jaroslav
collection PubMed
description The Auxin Binding Protein1 (ABP1) has been identified based on its ability to bind auxin with high affinity and studied for a long time as a prime candidate for the extracellular auxin receptor responsible for mediating in particular the fast non-transcriptional auxin responses. However, the contradiction between the embryo-lethal phenotypes of the originally described Arabidopsis T-DNA insertional knock-out alleles ( abp1-1 and abp1-1s) and the wild type-like phenotypes of other recently described loss-of-function alleles ( abp1-c1 and abp1-TD1) questions the biological importance of ABP1 and relevance of the previous genetic studies. Here we show that there is no hidden copy of the ABP1 gene in the Arabidopsis genome but the embryo-lethal phenotypes of abp1-1 and abp1-1s alleles are very similar to the knock-out phenotypes of the neighboring gene, BELAYA SMERT ( BSM). Furthermore, the allelic complementation test between bsm and abp1 alleles shows that the embryo-lethality in the abp1-1 and abp1-1s alleles is caused by the off-target disruption of the BSM locus by the T-DNA insertions. This clarifies the controversy of different phenotypes among published abp1 knock-out alleles and asks for reflections on the developmental role of ABP1.
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spelling pubmed-46428512015-11-30 Embryo-lethal phenotypes in early abp1 mutants are due to disruption of the neighboring BSM gene Michalko, Jaroslav Dravecká, Marta Bollenbach, Tobias Friml, Jiří F1000Res Research Article The Auxin Binding Protein1 (ABP1) has been identified based on its ability to bind auxin with high affinity and studied for a long time as a prime candidate for the extracellular auxin receptor responsible for mediating in particular the fast non-transcriptional auxin responses. However, the contradiction between the embryo-lethal phenotypes of the originally described Arabidopsis T-DNA insertional knock-out alleles ( abp1-1 and abp1-1s) and the wild type-like phenotypes of other recently described loss-of-function alleles ( abp1-c1 and abp1-TD1) questions the biological importance of ABP1 and relevance of the previous genetic studies. Here we show that there is no hidden copy of the ABP1 gene in the Arabidopsis genome but the embryo-lethal phenotypes of abp1-1 and abp1-1s alleles are very similar to the knock-out phenotypes of the neighboring gene, BELAYA SMERT ( BSM). Furthermore, the allelic complementation test between bsm and abp1 alleles shows that the embryo-lethality in the abp1-1 and abp1-1s alleles is caused by the off-target disruption of the BSM locus by the T-DNA insertions. This clarifies the controversy of different phenotypes among published abp1 knock-out alleles and asks for reflections on the developmental role of ABP1. F1000Research 2015-10-23 /pmc/articles/PMC4642851/ /pubmed/26629335 http://dx.doi.org/10.12688/f1000research.7143.1 Text en Copyright: © 2015 Michalko J et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Michalko, Jaroslav
Dravecká, Marta
Bollenbach, Tobias
Friml, Jiří
Embryo-lethal phenotypes in early abp1 mutants are due to disruption of the neighboring BSM gene
title Embryo-lethal phenotypes in early abp1 mutants are due to disruption of the neighboring BSM gene
title_full Embryo-lethal phenotypes in early abp1 mutants are due to disruption of the neighboring BSM gene
title_fullStr Embryo-lethal phenotypes in early abp1 mutants are due to disruption of the neighboring BSM gene
title_full_unstemmed Embryo-lethal phenotypes in early abp1 mutants are due to disruption of the neighboring BSM gene
title_short Embryo-lethal phenotypes in early abp1 mutants are due to disruption of the neighboring BSM gene
title_sort embryo-lethal phenotypes in early abp1 mutants are due to disruption of the neighboring bsm gene
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4642851/
https://www.ncbi.nlm.nih.gov/pubmed/26629335
http://dx.doi.org/10.12688/f1000research.7143.1
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