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Effect of Antibiotics on Gut Microbiota, Gut Hormones and Glucose Metabolism
OBJECTIVE: The gut microbiota has been designated as an active regulator of glucose metabolism and metabolic phenotype in a number of animal and human observational studies. We evaluated the effect of removing as many bacteria as possible by antibiotics on postprandial physiology in healthy humans....
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4643023/ https://www.ncbi.nlm.nih.gov/pubmed/26562532 http://dx.doi.org/10.1371/journal.pone.0142352 |
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author | Mikkelsen, Kristian H. Frost, Morten Bahl, Martin I. Licht, Tine R. Jensen, Ulrich S. Rosenberg, Jacob Pedersen, Oluf Hansen, Torben Rehfeld, Jens F. Holst, Jens J. Vilsbøll, Tina Knop, Filip K. |
author_facet | Mikkelsen, Kristian H. Frost, Morten Bahl, Martin I. Licht, Tine R. Jensen, Ulrich S. Rosenberg, Jacob Pedersen, Oluf Hansen, Torben Rehfeld, Jens F. Holst, Jens J. Vilsbøll, Tina Knop, Filip K. |
author_sort | Mikkelsen, Kristian H. |
collection | PubMed |
description | OBJECTIVE: The gut microbiota has been designated as an active regulator of glucose metabolism and metabolic phenotype in a number of animal and human observational studies. We evaluated the effect of removing as many bacteria as possible by antibiotics on postprandial physiology in healthy humans. METHODS: Meal tests with measurements of postprandial glucose tolerance and postprandial release of insulin and gut hormones were performed before, immediately after and 6 weeks after a 4-day, broad-spectrum, per oral antibiotic cocktail (vancomycin 500 mg, gentamycin 40 mg and meropenem 500 mg once-daily) in a group of 12 lean and glucose tolerant males. Faecal samples were collected for culture-based assessment of changes in gut microbiota composition. RESULTS: Acute and dramatic reductions in the abundance of a representative set of gut bacteria was seen immediately following the antibiotic course, but no changes in postprandial glucose tolerance, insulin secretion or plasma lipid concentrations were found. Apart from an acute and reversible increase in peptide YY secretion, no changes were observed in postprandial gut hormone release. CONCLUSION: As evaluated by selective cultivation of gut bacteria, a broad-spectrum 4-day antibiotics course with vancomycin, gentamycin and meropenem induced shifts in gut microbiota composition that had no clinically relevant short or long-term effects on metabolic variables in healthy glucose-tolerant males. TRIAL REGISTRATION: clinicaltrials.gov NCT01633762 |
format | Online Article Text |
id | pubmed-4643023 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-46430232015-11-18 Effect of Antibiotics on Gut Microbiota, Gut Hormones and Glucose Metabolism Mikkelsen, Kristian H. Frost, Morten Bahl, Martin I. Licht, Tine R. Jensen, Ulrich S. Rosenberg, Jacob Pedersen, Oluf Hansen, Torben Rehfeld, Jens F. Holst, Jens J. Vilsbøll, Tina Knop, Filip K. PLoS One Research Article OBJECTIVE: The gut microbiota has been designated as an active regulator of glucose metabolism and metabolic phenotype in a number of animal and human observational studies. We evaluated the effect of removing as many bacteria as possible by antibiotics on postprandial physiology in healthy humans. METHODS: Meal tests with measurements of postprandial glucose tolerance and postprandial release of insulin and gut hormones were performed before, immediately after and 6 weeks after a 4-day, broad-spectrum, per oral antibiotic cocktail (vancomycin 500 mg, gentamycin 40 mg and meropenem 500 mg once-daily) in a group of 12 lean and glucose tolerant males. Faecal samples were collected for culture-based assessment of changes in gut microbiota composition. RESULTS: Acute and dramatic reductions in the abundance of a representative set of gut bacteria was seen immediately following the antibiotic course, but no changes in postprandial glucose tolerance, insulin secretion or plasma lipid concentrations were found. Apart from an acute and reversible increase in peptide YY secretion, no changes were observed in postprandial gut hormone release. CONCLUSION: As evaluated by selective cultivation of gut bacteria, a broad-spectrum 4-day antibiotics course with vancomycin, gentamycin and meropenem induced shifts in gut microbiota composition that had no clinically relevant short or long-term effects on metabolic variables in healthy glucose-tolerant males. TRIAL REGISTRATION: clinicaltrials.gov NCT01633762 Public Library of Science 2015-11-12 /pmc/articles/PMC4643023/ /pubmed/26562532 http://dx.doi.org/10.1371/journal.pone.0142352 Text en © 2015 Mikkelsen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Mikkelsen, Kristian H. Frost, Morten Bahl, Martin I. Licht, Tine R. Jensen, Ulrich S. Rosenberg, Jacob Pedersen, Oluf Hansen, Torben Rehfeld, Jens F. Holst, Jens J. Vilsbøll, Tina Knop, Filip K. Effect of Antibiotics on Gut Microbiota, Gut Hormones and Glucose Metabolism |
title | Effect of Antibiotics on Gut Microbiota, Gut Hormones and Glucose Metabolism |
title_full | Effect of Antibiotics on Gut Microbiota, Gut Hormones and Glucose Metabolism |
title_fullStr | Effect of Antibiotics on Gut Microbiota, Gut Hormones and Glucose Metabolism |
title_full_unstemmed | Effect of Antibiotics on Gut Microbiota, Gut Hormones and Glucose Metabolism |
title_short | Effect of Antibiotics on Gut Microbiota, Gut Hormones and Glucose Metabolism |
title_sort | effect of antibiotics on gut microbiota, gut hormones and glucose metabolism |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4643023/ https://www.ncbi.nlm.nih.gov/pubmed/26562532 http://dx.doi.org/10.1371/journal.pone.0142352 |
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