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I-SceI-mediated double-strand DNA breaks stimulate efficient gene targeting in the industrial fungus Trichoderma reesei

Targeted integration of expression cassettes for enzyme production in industrial microorganisms is desirable especially when enzyme variants are screened for improved enzymatic properties. However, currently used methods for targeted integration are inefficient and result in low transformation frequ...

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Detalles Bibliográficos
Autores principales:	Ouedraogo, Jean Paul, Arentshorst, Mark, Nikolaev, Igor, Barends, Sharief, Ram, Arthur F. J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Berlin Heidelberg 2015
Materias:	Applied Genetics and Molecular Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4643118/ https://www.ncbi.nlm.nih.gov/pubmed/26272087 http://dx.doi.org/10.1007/s00253-015-6829-1

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